[1]程金来,梁 丹,冷 静,等.HPLC法测定加味逍遥散复方中丹参酮ⅡA的含量[J].医学信息,2020,33(02):165-166,171.[doi:10.3969/j.issn.1006-1959.2020.02.052]
　CHENG Jin-lai,LIANG Dan,LENG Jing,et al.Determination of Tanshinone ⅡA in Jiawei Xiaoyao San Compound by HPLC[J].Medical Information,2020,33(02):165-166,171.[doi:10.3969/j.issn.1006-1959.2020.02.052]
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HPLC法测定加味逍遥散复方中丹参酮ⅡA的含量()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年02期
页码:
165-166,171
栏目:
诊疗技术
出版日期:
2020-01-15

文章信息/Info

Title:
Determination of Tanshinone ⅡA in Jiawei Xiaoyao San Compound by HPLC
文章编号:
1006-1959（2020）02-0165-03
作者:
程金来; 梁 丹; 冷 静; 曹子茵; 夏 猛
（广西中医药大学基础医学院/广西特色实验动物病症模型重点实验室/广西高发传染病中西医结合转化医学重点实验室培育基地，广西 南宁 530200）
Author(s):
CHENG Jin-lai; LIANG Dan; LENG Jing; CAO Zi-yin; XIA Meng
(Basic Medical College of Guangxi University of Traditional Chinese Medicine/Guangxi Key Laboratory of Experimental Animal Disease Models with Characteristics/Guangxi Key Laboratory of Highly Infectious Diseases Integrated Laboratory of Integrated Traditi
关键词:
加味逍遥散; 含量测定; 丹参酮ⅡA; HPLC
Keywords:
Jiawei Xiaoyao San; Content determination; Tanshinone IIA; HPLC
分类号:
R286.0
DOI:
10.3969/j.issn.1006-1959.2020.02.052
文献标志码:
A
摘要:
目的 建立加味逍遥散复方中丹参酮ⅡA的测定方法。方法 采用十八烷基硅烷键合硅胶柱（inertsil ODS-3柱 250 mm×4.6 mm，5 μm）；流动相：乙腈-0.1%磷酸，进行梯度洗脱；检测波长：270 nm；流速：1.0 ml/min；柱温：20℃。结果 丹参酮ⅡA在0.0001~0.0005 μg范围内呈现良好的线性关系[r=0.9991，平均回收率为97.36%（RSD=1.38%，n=6）]。结论 本方法专属性强，准确度高，重现性良好，可用于加味逍遥散的质量控制。
Abstract:
Objective To establish a method for determination of Tanshinone ⅡA in Jiawei Xiaoyao San compound. Methods An octadecylsilane bonded silica column (inertsil ODS-3 column 250 mm×4.6 mm, 5 μm) was used; Mobile phase: acetonitrile-0.1% phosphoric acid, gradient elution; detection wavelength: 270 nm; flow rate: 1.0 ml/min; column temperature: 20℃. Results Tanshinone IIA showed a good linear relationship in the range of 0.0001 to 0.0005 μg [r=0.9991, the average recovery was 97.36% (RSD=1.38%, n=6)]. Conclusion This method has strong specificity, high accuracy and good reproducibility, and can be used for the quality control of Jiawei Xiaoyao San.

参考文献/References:

[1]杨超.加味逍遥散联合经皮射频消融术治疗21例原发性肝癌（肝郁脾虚型）的临床疗效观察[D].湖北中医药大学,2012. [2]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:105.

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更新日期/Last Update: 2020-01-15