[1]谢 晨,郭仲谦,张广政,等.郑州农村汉族男性27个Y-STR遗传多态性及多民族间遗传距离的比较[J].医学信息,2020,33(03):74-78.[doi:10.3969/j.issn.1006-1959.2020.03.022]
　XIE Chen,GUO Zhong-qian,ZHANG Guang-zheng,et al.Comparison of 27 Y-STR Genetic Polymorphisms and Genetic Distance Among Ethnic Groups in Rural Han Males in Zhengzhou[J].Medical Information,2020,33(03):74-78.[doi:10.3969/j.issn.1006-1959.2020.03.022]
点击复制

郑州农村汉族男性27个Y-STR遗传多态性及多民族间遗传距离的比较()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年03期
页码:
74-78
栏目:
论著
出版日期:
2020-02-01

文章信息/Info

Title:
Comparison of 27 Y-STR Genetic Polymorphisms and Genetic Distance Among Ethnic Groups in Rural Han Males in Zhengzhou
文章编号:
1006-1959（2020）03-0074-05
作者:
谢 晨; 郭仲谦; 张广政; 等
（1.郑州大学基础医学院法医学系，河南 郑州 450000；2.郑州市公安局犯罪侦查局刑事科学技术研究所，河南 郑州 450000；3.河南省直第三人民医院<司法鉴定中心>，河南 郑州 450000；4.公安部物证鉴定中心，北京 100038）
Author(s):
XIE Chen1; 2; GUO Zhong-qian1; 3; ZHANG Guang-zheng1; WANG Lei2; ZENG Zhao-shu1; LIU Kai-hui4
(1.Department of Forensic Medicine,School of Basic Medical Sciences,Zhengzhou University, Zhengzhou 450000,Henan,China;2.Institute of Criminal Science and Technology,Criminal Investigation Bureau,Zhengzhou Public Security Bureau,Zhengzhou 450000,Henan,Chi
关键词:
法医物证学; 遗传距离; Y-STR基因座; 单体型; 遗传多态性; 郑州; 汉族; 男性
Keywords:
Forensic physical evidence; Genetic distance; Y-STR locus; Haplotype; Genetic polymorphism; Zhengzhou; Han; Male
分类号:
D919
DOI:
10.3969/j.issn.1006-1959.2020.03.022
文献标志码:
A
摘要:
目的 比较郑州农村汉族男性27个Y-STR遗传多态性及多民族间的遗传距离。方法 采用Yfiler Plus试剂盒对27个Y-STR基因座进行扩增，3500 xL遗传分析仪进行基因分型，统计27个Y-STR基因座的等位基因频率及基因差异度等遗传学参数。以YHRD网站的分子方差分析（AMOVA）程序分析不同省份汉族及不同少数民族男性人群的遗传距离，以该网站的多维尺度分析（MDS）程序绘制MDS图。结果 郑州农村汉族男性中共540个不同的单倍型，27个Y-STR基因座所组成的HD值为0.999974。基因型数据分析显示，27个Y-STR中DYS385b、DYS449、DYS518和DYS627表现出高基因差异度（GD＞0.85），而DYS391和DYS438表现出低基因差异度（GD＜0.5）。在与其他省份汉族男性的遗传距离比较，郑州农村汉族男性与陕西汉族男性遗传距离最近（Rst=0.0028），与广西汉族遗传距离最远（Rst=0.0891）；在与其他省份少数民族男性的遗传距离比较，郑州农村汉族男性与陕西回族男性的遗传距离最近（Rst=0.01526），与四川藏族的遗传距离最远（Rst=0.2873）。结论 Yfiler Plus的27个Y-STR基因座在郑州农村汉族男性中具有良好的多态性，可用于区分郑州农村汉族无关男性个体。由于农村地区一般为多代世居于此，故本结果可视为郑州本地男性代表性数据，可为本地区的法医学应用提供基础数据。
Abstract:
Objective To compare 27 Y-STR genetic polymorphisms and genetic distances among ethnic groups in rural Han males in Zhengzhou. Methods The Yfiler Plus kit was used to amplify 27 Y-STR loci, the 3500 xL genetic analyzer was used for genotyping, and the genetic parameters such as allele frequency and gene difference were counted for 27 Y-STR loci. The molecular distance analysis (AMOVA) program of the YHRD website was used to analyze the genetic distance of the Han and different minority male populations in different provinces, and the MDS map was drawn using the website’s multidimensional scale analysis (MDS) program.Results There were 540 different haplotypes in rural Han males in Zhengzhou, and the HD value composed of 27 Y-STR loci was 0.999974. Analysis of genotype data showed that among 27 Y-STR, DYS385b, DYS449, DYS518, and DYS627 showed high genetic differences (GD>0.85), while DYS391 and DYS438 showed low genetic differences (GD<0.5). In comparison with the genetic distance of Han males in other provinces, the rural Han males in Zhengzhou have the closest genetic distance to the Han males in Shaanxi (Rst=0.0028), and the genetic distance to the Han population in Guangxi is the furthest (Rst=0.0891). For distance comparison, the genetic distance between Han males in rural Zhengzhou and Hui males in Shaanxi is the closest (Rst=0.01526), and the genetic distance between Sichuan males and Sichuan Tibetans is the longest (Rst=0.2873).Conclusion The 27 Y-STR loci of Yfiler Plus have good polymorphism in Han males in rural Zhengzhou, which can be used to distinguish unrelated male individuals in rural Zhengzhou Han. Since the rural areas are usually here for many generations, this result can be regarded as the representative data of local males in Zhengzhou, which can provide basic data for forensic applications in this area.

参考文献/References:

[1]邓盼,江丽,马泉,等.广西6个民族24个Y-STR基因座遗传多态性及群体遗传结构分析[J].刑事技术,2018,43(3):198-201. [2]Nei M,Tajima F.DNA polymorphism detectable by restriction endonucleases[J].Genetics,1981,97(1):145-163. [3]Willuweit S,Roewer L.The new Y Chromosome Haplotype Reference Database[J].Forensic Sci Int Genet,2015(15):43-48. [4]Nothnagel M,Fan G,Guo F,et al.Revisiting the male genetic landscape of China:a multi-center study of almost 38,000 Y-STR haplotypes[J].Human genetics,2017,136(5):485-497. [5]Li L,Yu G,Li S,et al.Genetic analysis of 17 Y-STR loci from 1019 individuals of six Han populations in East China[J].Forensic Science International:Genetics,2016(20):101-102. [6]Wang H,Ba H,Yang C,et al.Inner and inter population structure construction of Chinese Jiangsu Han population based on Y23 STR system[J].PLoS One,2017,12(7):e0180921. [7]Wang Y,Zhang Y,Zhang C,et al.Genetic polymorphisms and mutation rates of 27 Y-chromosomal STRs in a Han population from Guangdong Province,Southern China[J].Forensic Science International:Genetics,2016(21):5-9. [8]Guan T,Song X,Xiao C,et al.Analysis of 23 Y-STR loci in Chinese Jieyang Han population[J].International Journal of Legal Medicine,2019:1-3. [9]Zhou Y,Shao C,Li L,et al.Genetic analysis of 29 Y-STR loci in the Chinese Han population from Shanghai[J].Forensic Science International:Genetics,2018(32):e1-e4. [10]Zhang S,Tian H,Wang Z,et al.Development of a new 26plex Y-STRs typing system for forensic application[J].Forensic Science International:Genetics,2014(13):112-120. [11]Jiang W,Gong Z,Rong H,et al.Population genetics of 26 Y-STR loci for the Han ethnic in Hunan province,China[J].International Journal of Legal Medicine,2017,131(1):115-117. [12]Yao HB,Wang CC,Tao X,et al.Genetic evidence for an East Asian origin of Chinese Muslim populations Dongxiang and Hui[J].Scientific Reports,2016(6):38656. [13]Zhao Q,Bian Y,Zhang S,et al.Population genetics study using 26 Y-chromosomal STR loci in the Hui ethnic group in China[J].Forensic Science International:Genetics,2017(28):e26-e27. [14]Xie M,Song F,Li J,et al.Genetic substructure and forensic characteristics of Chinese Hui populations using 157 Y-SNPs and 27 Y-STRs[J].Forensic Science International:Genetics,2019(41):11-18. [15]Zhu B,Deng Y,Zhang F,et al.Genetic analysis for Y chromosome short tandem repeat haplotypes of Chinese Han population residing in the Ningxia province of China[J].Journal of Forensic Sciences,2006,51(6):1417-1420. [16]Ou X,Wang Y,Liu C,et al.Haplotype analysis of the polymorphic 40 Y-STR markers in Chinese populations[J].Forensic Science International:Genetics,2015(19):255-262. [17]Bai R,Liu Y,Lv X,et al.Genetic polymorphisms of 17 Y chromosomal STRs in She and Manchu ethnic populations from China[J].Forensic Science International:Genetics,2016(22):e12-e14. [18]He J,Guo F.Population genetics of 17 Y-STR loci in Chinese Manchu population from Liaoning province,Northeast China[J].Forensic Science International:Genetics,2013,7(3):e84-e85. [19]Chen P,He G,Zou X,et al.Genetic diversities and phylogenetic analyses of three Chinese main ethnic groups in southwest China:A Y-Chromosomal STR study[J].Scientific Reports,2018,8(1):15339. [20]Song F,Xie M,Xie B,et al.Genetic diversity and phylogenetic analysis of 29 Y-STR loci in the Tibetan population from Sichuan Province,Southwest China[J].International Journal of Legal Medicine,2019:1-4. [21]Bian Y,Zhang S,Zhou W,et al.Analysis of genetic admixture in Uyghur using the 26 Y-STR loci system[J].Scientific Reports,2016(6):19998. [22]Shan W,Ablimit A,Zhou W,et al.Genetic polymorphism of 17 Y chromosomal STRs in Kazakh and Uighur populations from Xinjiang,China[J].International Journal of Legal Medicine,2014,128(5):743-744. [23]He GL,Chen PY,Zou X,et al.Genetic polymorphism investigation of the Chinese Yi minority using PowerPlex? Y23 STR amplification system[J].International Journal of Legal Medicine,2017,131(3):663-666. [24]Luo H,Song F,Zhang L,et al.Genetic polymorphism of 23 Y-STR loci in the Zhuang minority population in Guangxi of China[J].International Journal of Legal Medicine,2015,129(4):737-738. [25]Guo F,Li J,Chen K,et al.Population genetic data for 27 Y-STR loci in the Zhuang ethnic minority from Guangxi Zhuang Autonomous Region in the south of China[J].Forensic Science International:Genetics,2017(27):182-183. [26]Fu X,Fu Y,Liu Y,et al.Genetic polymorphisms of 26 Y-STR loci in the Mongolian minority from Horqin district,China[J].International Journal of Legal Medicine,2016,130(4):941-946. [27]Xu H,Wang CC,Shrestha R,et al.Inferring population structure and demographic history using Y-STR data from worldwide populations[J].Molecular Genetics and Genomics,2015,290(1):141-150. [28]刘亚举,张俊涛,史绍杏,等.河南汉族人群27个Y-STR基因座遗传多态性[J].刑事技术,2014,39(4):18-20. [29]杜若甫,肖春杰,Cavalli-Sforza LL.用38个基因座的基因频率计算中国人群间遗传距离[J].中国科学C辑,1998,25(1):83-89. [30]王文菲,吕自力,张勇,等.新疆哈萨克族15个常染色体STR位点检测及40个中国人群遗传关系[J].郑州大学学报(医学版),2017,15(3):8-9. [31]宋土生,黄辰,司履生,等.应用网络STR生物信息对中国14个人群遗传距离的研究[J].西安交通大学学报,2003,24(1):1-4. [32]Chu JY,Huang W,Kuang SQ,et al.Genetic relationship of populations in China[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(20):11763-11768.

相似文献/References:

[1]林钻芳.20个常染色体STR基因座的突变分析[J].医学信息,2020,33(04):133.[doi:10.3969/j.issn.1006-1959.2020.04.042]
　LIN Zuan-fang.Mutation Analysis of 20 Autosomal STR Loci[J].Medical Information,2020,33(03):133.[doi:10.3969/j.issn.1006-1959.2020.04.042]

更新日期/Last Update: 2020-02-01