[1]郭 卉,董瑶佳,张启贵,等.钩端螺旋体引起巨噬细胞炎症反应的研究[J].医学信息,2020,33(20):57-59.[doi:10.3969/j.issn.1006-1959.2020.20.016]
　GUO Hui,DONG Yao-jia,ZHANG Qi-gui,et al.Study on Leptospira-induced Inflammation of Macrophages[J].Medical Information,2020,33(20):57-59.[doi:10.3969/j.issn.1006-1959.2020.20.016]
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钩端螺旋体引起巨噬细胞炎症反应的研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年20期
页码:
57-59
栏目:
论著
出版日期:
2020-10-15

文章信息/Info

Title:
Study on Leptospira-induced Inflammation of Macrophages
文章编号:
1006-1959（2020）20-0057-03
作者:
郭 卉; 董瑶佳; 张启贵; 等
（1.九江市第一人民医院检验科，江西 九江 332000； 2.九江市妇幼保健医院检验科，江西 九江 332000； 3.南昌大学第一附属医院，江西 南昌 330006）
Author(s):
GUO Hui; DONG Yao-jia; ZHANG Qi-gui; et al
(1.Department of Laboratory Medicine,the First People’s Hospital of Jiujiang City,Jiujiang 332000,Jiangxi,China; 2.Department of Laboratory Medicine,Jiujiang Maternal and Child Health Hospital,Jiujiang 332000, Jiangxi,China; 3.the First Affiliated Hospita
关键词:
钩端螺旋体; 巨噬细胞; 炎症反应
Keywords:
Leptospira; Macrophages; Inflammation response
分类号:
S855
DOI:
10.3969/j.issn.1006-1959.2020.20.016
文献标志码:
A
摘要:
目的 研究钩端螺旋体56601株、JDL03株和JDL10株刺激金黄地鼠腹腔巨噬细胞后炎症因子的表达情况。方法 分别采用钩端螺旋体56601株、JDL03株与JDL10株感染金黄地鼠腹腔巨噬细胞后，取上清，采用ELISA法检测3个细胞株中TNF-α、IL-1β和IL-6的表达水平。结果 培养24 h后，感染56601株的金黄地鼠腹腔巨噬细胞中TNF-α、IL-1β和IL-6的表达水平高于感染JDL03株和JDL10株，差异有统计学意义（P＜0.05）。结论 钩端螺旋体56601株能在巨噬细胞中引起较强的炎症反应，而JDL03株和JDL10株引起的炎症反应较轻，可用于后期筛选疫苗靶标。
Abstract:
Objective To study the expression of inflammatory factors after Leptospira strain 56601, JDL03 strain and JDL10 strain stimulated the peritoneal macrophages of golden hamster.Methods After infecting golden hamster peritoneal macrophages with Leptospira 56601, JDL03 and JDL10, the supernatant was taken and the expression of TNF-α, IL-1β and IL-6 in the three cell lines was detected by ELISA level.Results After 24 h of culture, the expression levels of TNF-α, IL-1β and IL-6 in the peritoneal macrophages of golden hamster infected with 56601 were higher than those infected with JDL03 and JDL10,the difference was statistically significant (P＜0.05).Conclusion The Leptospira 56601 strain could cause a strong inflammatory response in macrophages, while the JDL03 and JDL10 strains cause a milder inflammatory response, which could be used for later screening of vaccine targets.

参考文献/References:

[1]Adler B.History of Leptospirosis and Leptospira[J].Current Topics in Microbiology&Immunology,2015(387):1. [2]Fernandes LG,Siqueira GH,Teixeira ARF,et al.Leptospira spp:Novel insights into host-pathogen interactions[J].Veterinary Immunology&Immunopathology,2015:S0165242715300271. [3]Levett PN.Leptospirosis[J].Clinical Microbiology Reviews,2001,14(2):296. [4]罗依惠,吴亦斐,Munyampundu JP.问号钩端螺旋体过氧化物还原酶AhpC在氧化应激中的功能研究[J].中华微生物学和免疫学杂志,2018,38(3):193-198. [5]聂志文,梁振山,曾令兵.问号钩体56601株外分泌蛋白功能研究[J].实验与检验医学,2015(2):8-10,17. [6]范仕郡,刘鑫,黄敏,等.小鼠腹腔巨噬细胞的快速提取及培养[J].局解手术学杂志,2015,24(2):8,130-131. [7]潘敏楠,徐国英.钩端螺旋体EMJH培养基实验室应用[J].中国人兽共患病学报,2020(4):317-319. [8]范霞,夏碧丽,吕霖,等.小鼠巨噬细胞对钩端螺旋体56606v和56606a的吞噬及炎症应答的比较研究[J].上海交通大学学报(医学版),2019,39(1):26-30. [9]Aurélie M,Djelouadji Z,Lattard V,et al.Overview of laboratory methods to diagnose Leptospirosis and to identify and to type leptospires[J].International Microbiology the Official Journal of the Spanish Society for Microbiology,2017,20(4):184. [10]Garcia LE,de Araújo Junior EC,Melo LM,et al.Characterization of the microtranscriptome of macrophages infected with virulent,attenuated and saprophyte strains of Leptospira spp[J].Plos Neglected Tropical Diseases,2018,12(7):e0006621. [11]Zeng LB,Zhuang XR,Huang LL,et al.Comparative subproteome analysis of three representative Leptospira interrogans vaccine strains reveals cross-reactive antigens and novel virulence determinants[J].J Proteomics,2015(112):27-37. [12]Zeng L,Zhang Y,Zhu Y,et al.Extracellular proteome analysis of Leptospira interrogans serovar Lai[J].OMICS,2013,17(10):527-535. [13]Cagliero J,Villanueva S,Matsui M.Leptospirosis Pathophysiology:Into the Storm of Cytokines[J].Front Cell Infect Microbiol,2018(8):204. [14]Domingos RH,Pavanel EB,Nakajima E,et al.Resistance of mice to Leptospira infection and correlation with chemokine response[J].Immunobiology,2017,222(11):1004-1013. [15]王铭,陈红,刘英,等.问号钩端螺旋体对THP-1和J774A.1细胞NLRP3炎症小体活化的影响[J].中华微生物学和免疫学杂志,2016,36(12):894-899. [16]Silva P,Lauretti-Ferreira F,Caldas DLM,et al.Phagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokines[J].BMC Microbiol,2019,19(1):4. [17]Hirano S,Zhou Q,Furuyama A,et al.Differential Regulation of IL-1beta and IL-6 Release in Murine Macrophages[J].Inflammation,2017,40(6):1933-1943. [18]Nancy C,Emilia S,María FF,et al.Leptospira species promote a pro-inflammatory phenotype in human neutrophils[J].Cellular Microbiology,2019,21(2):e12990.

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更新日期/Last Update: 1900-01-01