[1]谭晓君,杨心怡,张雪杨,等.生脉散对H2O2损伤PC12细胞保护作用的研究[J].医学信息,2023,36(11):86-90.[doi:10.3969/j.issn.1006-1959.2023.11.015]
　TAN Xiao-jun,YANG Xin-yi,ZHANG Xue-yang,et al.Study on the Protective Effect of Shengmai-san on PC12 Cells Damaged by H2O2[J].Journal of Medical Information,2023,36(11):86-90.[doi:10.3969/j.issn.1006-1959.2023.11.015]
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生脉散对H2O2损伤PC12细胞保护作用的研究()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年11期
页码:
86-90
栏目:
论著
出版日期:
2023-06-01

文章信息/Info

Title:
Study on the Protective Effect of Shengmai-san on PC12 Cells Damaged by H2O2
文章编号:
1006-1959（2023）11-0086-05
作者:
谭晓君; 杨心怡; 张雪杨; 等
（1.广东医科大学药学院，广东 东莞 523808；2.深圳罗湖人民医院医学实验室，广东 深圳 518002）
Author(s):
TAN Xiao-jun; YANG Xin-yi; ZHANG Xue-yang; et al.
(1.College of Pharmacy,Guangdong Medical University,Dongguan 523808,Guangdong,China;2.Medical Laboratory of Shenzhen Luohu People’s Hospital,Shenzhen 518002,Guangdong,China)
关键词:
生脉散; H2O2; 氧化损伤; PC12细胞
Keywords:
Shengmai-san;  H2O2; Oxidative damage; PC12 cells
分类号:
R285
DOI:
10.3969/j.issn.1006-1959.2023.11.015
文献标志码:
A
摘要:
目的 探究生脉散（SMS）对PC12细胞增殖的影响和经H2O2损伤后对PC12细胞的保护作用。方法 用H2O2处理小鼠PC12细胞，构建氧化应激损伤细胞模型；通过CCK8法检测SMS对PC12细胞增殖的影响以及SMS对H2O2诱导损伤后的PC12细胞存活率的影响；将PC12细胞分为空白对照组、H2O2损伤模型组、SMS低剂量组、SMS中剂量组、SMS高剂量组，对照组不做处理，其他组均采用H2O2诱导损伤，SMS低、中、高剂量组则分别加入浓度为0.1、1.0、5.0 g/ml的SMS，然后检测胞内外乳酸脱氢酶（LDH）、丙二醛（MDA）、超氧化物歧化酶（SOD）的表达水平。通过蛋白质印迹法检测SMS对PC12细胞的核因子E2相关因子2（Nrf-2）蛋白表达水平的影响。结果 SMS各含量组能促进正常PC12细胞的增殖、提高氧化损伤的PC12细胞的存活率；能使细胞外LDH活性减弱、MDA含量下降，SOD活力增强，差异有统计学意义（P＜0.05）；SMS可上调PC12损伤细胞中Nrf-2蛋白的表达，差异有统计学意义（P＜0.05）。结论 SMS能促进PC12细胞的增殖且对H2O2诱导的细胞损伤有保护作用，其机制可能与增强核内Nrf-2蛋白表达有关。
Abstract:
Objective To investigate the effect of Shengmai-san (SMS) on the proliferation of PC12 cells and the protective effect of SMS on PC12 cells after H2O2 injury.Methods PC12 cells were treated with H2O2 to construct a model of oxidative stress injury; the effect of SMS on the proliferation of PC12 cells and the effect of SMS on the survival rate of PC12 cells after H2O2-induced injury were detected by CCK8 method. PC12 cells were divided into blank control group, H2O2 injury model group, SMS low dose group, SMS medium dose group and SMS high dose group, while the control group was not treated, and the other groups were induced by H2O2 injury. SMS low, medium and high dose groups were added with SMS at concentrations of 0.1, 1.0 and 5.0 g/ml, and then the expression levels of intracellular and extracellular lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) were detected. The effect of SMS on the expression of nuclear factor E2-related factor 2 (Nrf-2) protein in PC12 cells was detected by Western blotting.Results Different concentrations of SMS groups could promote the proliferation of normal PC12 cells and increase the survival rate of oxidative damaged PC12 cells, meanwhile the activity of extracellular LDH was decreased, the content of MDA was decreased, and the activity of SOD was increased, the difference was statistically significant (P＜0.05). SMS could up-regulate the expression of Nrf-2 protein in PC12 damaged cells, and the difference was statistically significant (P＜0.05).Conclusion SMS can promote the proliferation of PC12 cells and protect against H2O2-induced cell injury, and the mechanism may be related to the enhancement of Nrf-2 protein expression in the nucleus.

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更新日期/Last Update: 1900-01-01