[1]陈中凯,伍贤权,敬家辉,等.青藤碱调控LncRNA-HEIH表达抑制人食管癌细胞迁移和侵袭[J].医学信息,2024,37(19):62-67.[doi:10.3969/j.issn.1006-1959.2024.19.010]
　CHEN Zhongkai,WU Xianquan,JING Jiahui,et al.Sinomenine Inhibits Migration and Invasion of Human Esophageal Cancer Cells by Regulating LncRNA-HEIH Expression[J].Journal of Medical Information,2024,37(19):62-67.[doi:10.3969/j.issn.1006-1959.2024.19.010]
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青藤碱调控LncRNA-HEIH表达抑制人食管癌细胞迁移和侵袭()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年19期
页码:
62-67
栏目:
论著
出版日期:
2024-10-01

文章信息/Info

Title:
Sinomenine Inhibits Migration and Invasion of Human Esophageal Cancer Cells by Regulating LncRNA-HEIH Expression
文章编号:
1006-1959（2024）19-0062-06
作者:
陈中凯; 伍贤权; 敬家辉; 龙春晖; 李 龙; 陈伟毅
湖南医药学院医学院，湖南 怀化 418000
Author(s):
CHEN Zhongkai; WU Xianquan; JING Jiahui; LONG Chunhui; LI Long; CHEN Weiyi
Medical School of Hunan University of Medicine,Huaihua 418000,Hunan,China
关键词:
青藤碱; HEIH; 迁移; 侵袭; 食管癌
Keywords:
Sinomenine; HEIH; Migration; Invasion; Esophageal cancer
分类号:
R285.5
DOI:
10.3969/j.issn.1006-1959.2024.19.010
文献标志码:
A
摘要:
目的 探讨青藤碱（SIN）对食管癌细胞迁移、侵袭的影响及对LncRNA-HEIH的调控作用。方法 采用不同浓度青藤碱处理食管癌TE-1细胞，si-NC、si-HEIH分别转染TE-1细胞，pcDNA、pcDNA-HEIH分别转染TE-1细胞后加入青藤碱处理细胞；CCK8检测细胞活力，划痕实验检测细胞迁移率，Transwell实验检测侵袭细胞数，qRT-PCR检测HEIH表达量。结果 青藤碱25、50、100 mg/L对TE-1细胞活力无明显影响（P＞0.05），在青藤碱25、50、100 mg/L的作用下，TE-1细胞迁移率和侵袭细胞数显著降低，差异有统计学意义（P＜0.01），在青藤碱25、50、100 mg/L的作用下，TE-1细胞HEIH表达显著降低，差异有统计学意义（P＜0.01）。转染si-HEIH可显著降低TE-1细胞迁移率和侵袭细胞数，差异有统计学意义（P＜0.01），过表达HEIH可显著逆转青藤碱降低TE-1细胞迁移率和侵袭细胞数的作用（P＜0.01）。结论 青藤碱通过抑制HEIH表达，抑制食管癌细胞迁移和侵袭。
Abstract:
Objective To investigate the effect of sinomenine (SIN) on the migration and invasion of esophageal cancer cells and the regulation of LncRNA-HEIH.Methods Esophageal cancer TE-1 cells were treated with different concentrations of sinomenine. TE-1 cells were transfected with si-NC and si-HEIH, respectively. TE-1 cells were transfected with pcDNA and pcDNA-HEIH, respectively, and then treated with sinomenine. CCK8 was used to detect cell viability, scratch test was used to detect cell migration rate, Transwell test was used to detect the number of invasive cells, and qRT-PCR was used to detect HEIH expression.Results Sinomenine 25, 50 and 100 mg/L had no significant effect on the viability of TE-1 cells (P>0.05). Under the action of sinomenine 25, 50 and 100 mg/L, the migration rate and invasive cell number of TE-1 cells decreased significantly (P<0.01). Under the action of sinomenine 25, 50 and 100 mg/L, the expression of HEIH in TE-1 cells decreased significantly (P<0.01). Transfection of si-HEIH significantly reduced the migration rate and the number of invasive cells of TE-1 cells (P<0.01). Overexpression of HEIH significantly reversed the effect of sinomenine on reducing the migration rate and the number of invasive cells of TE-1 cells (P<0.01).Conclusion Sinomenine inhibits the migration and invasion of esophageal cancer cells by inhibiting the expression of HEIH.

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更新日期/Last Update: 1900-01-01