[1]陆静宇,陈世娟,王楚睿,等.清热安胎膏改善大鼠妊娠期龈炎及对SOD、CAT活性的影响[J].医学信息,2025,38(08):65-69.[doi:10.3969/j.issn.1006-1959.2025.08.013]
　LU Jingyu,CHEN Shijuan,WANG Churui,et al.Effect of Qingre Antai Paste on the Improvement of Gingivitis and the Activity of SOD and CAT in Rats During Pregnancy[J].Journal of Medical Information,2025,38(08):65-69.[doi:10.3969/j.issn.1006-1959.2025.08.013]
点击复制

清热安胎膏改善大鼠妊娠期龈炎及对SOD、CAT活性的影响()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
38卷
期数:
2025年08期
页码:
65-69
栏目:
论著
出版日期:
2025-04-15

文章信息/Info

Title:
Effect of Qingre Antai Paste on the Improvement of Gingivitis and the Activity of SOD and CAT in Rats During Pregnancy
文章编号:
1006-1959（2025）08-0065-05
作者:
陆静宇; 陈世娟; 王楚睿; 康 静
湖南中医药大学第一附属医院口腔科，湖南 长沙 410007
Author(s):
LU Jingyu;  CHEN Shijuan;  WANG Churui;  KANG Jing
Department of Stomatology, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan, China
关键词:
妊娠期龈炎; 清热安胎膏; 氧化应激
Keywords:
Pregnancy gingivitis;  Qingre Antai paste;  Oxidative stress
分类号:
R276.8
DOI:
10.3969/j.issn.1006-1959.2025.08.013
文献标志码:
A
摘要:
目的 观察中药清热安胎膏对大鼠妊娠期龈炎的疗效、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性的影响。方法 从北京维通利华公司购入24只9周龄SPF级别的SD雌鼠，12只雄鼠，采用简单随机分组的方法将雌鼠均等随机分配至四个实验组别：空白对照组、模型组、阳性药物对照组及中药组，每组包含6只大鼠。适应性喂养后构建实验性龈炎，7 d后合笼受孕，受孕3 d后予以分组处理。并拍摄记录各组大鼠造模后和分组处理后牙龈状态。空白对照组：不做任何处理，常规受孕；模型组：构建实验性龈炎后受孕；阳性药物对照组：构建实验性龈炎，受孕后龈沟处予以碘甘油；中药组：构建实验性龈炎，受孕后龈沟处予以填塞清热安胎膏。分组处理后，切取牙龈组织，酶联免疫吸附试验（ELISA）测定实验大鼠牙龈组织中SOD和CAT的活性水平。结果 大鼠造模后，双下门齿牙龈出现明显充血，红肿等情况。妊娠期各组大鼠处理后牙龈红肿程度：模型组＞中药组≥阳性药物对照组＞空白对照组。与空白对照组相比，模型组、阳性药物对照组、中药组SOD、CAT活性降低（P＜0.05）；中药组SOD、CAT活性高于模型组（P＜0.05）。结论 清热安胎膏可以有效提高大鼠妊娠期龈炎中SOD、CAT活性，改善氧化应激，减轻牙龈组织红肿及损伤，有望成为治疗妊娠期龈炎的新方法。
Abstract:
Objective To observe the effect of Qingre Antai paste on the activity of superoxide dismutase (SOD) and catalase (CAT) in rats with gingivitis during pregnancy. Methods A total of 24 9-week-old SPF SD female rats and 12 male rats were purchased from Beijing Charles River Company. Female rats were randomly divided into four experimental groups: blank control group, model group, positive drug control group and traditional Chinese medicine group, with 6 rats in each group. After adaptive feeding, experimental gingivitis was constructed. After 7 days, they were conceived in a cage, and after 3 days of conception, they were grouped. The gingival state of rats in each group after modeling and group treatment was recorded. Blank control group: without any treatment, conventional conception; model group: pregnancy after experimental gingivitis was constructed; positive drug control group: experimental gingivitis was constructed, and iodine glycerin was given at the gingival sulcus after conception; traditional Chinese medicine group: experimental gingivitis was constructed, and Qingre Antai paste was filled in the gingival sulcus after conception. After grouping treatment, the gingival tissue was cut, and the activity levels of SOD and CAT in the gingival tissue of the experimental rats were determined by enzyme-linked immunosorbent assay (ELISA). Results After modeling, the gums of the lower incisors of the rats showed obvious congestion, redness and swelling. The degree of gingival swelling after treatment in each group of rats during pregnancy: model group>traditional Chinese medicine group≥positive drug control group>blank control group. Compared with the blank control group, the activity of SOD and CAT in the model group, the positive drug control group and the traditional Chinese medicine group decreased (P<0.05). The activities of SOD and CAT in the traditional Chinese medicine group were higher than those in the model group (P<0.05). Conclusion Qingre Antai paste can effectively improve the activity of SOD and CAT in rats with gingivitis during pregnancy, improve oxidative stress, and reduce the swelling and damage of gingival tissue. It is expected to become a new method for the treatment of gingivitis during pregnancy.

参考文献/References:

[1]Clement C,Bourgeois D,Vitiello F,et al.Efficacy of Sonic-Powered and Manual Toothbrushes on Plaque-Induced Gingivitis in Pregnant Women: A Randomized Controlled Trial[J].Methods Protoc,2023,6(5):99.[2]El Hassan NKB,Ramadan AM.Interleukin 1 beta and its association with the periodontal health of pregnant women[J].J Taibah Univ Med Sci,2023,18(6):1376-1383.[3]Saadaoui M,Singh P,Al Khodor S.Oral microbiome and pregnancy: A bidirectional relationship[J].J Reprod Immunol,2021,145:103293.[4]Shang J,Liu H,Zheng Y,et al.Role of oxidative stress in the relationship between periodontitis and systemic diseases[J].Front Physiol,2023,14:1210449.[5]Grosso C,Santos M,Barroso MF.From Plants to Psycho-Neurology: Unravelling the Therapeutic Benefits of Bioactive Compounds in Brain Disorders[J].Antioxidants (Basel),2023,12(8):1603.[6]Liu B,Li L,Liu G,et al.Baicalein attenuates cardiac hypertrophy in mice via suppressing oxidative stress and activating autophagy in cardiomyocytes[J].Acta Pharmacol Sin,2021,42(5):701-714.[7]宋楠楠,刘超,姚锐,等.清热药知母及其配伍防治糖尿病肾病的效应机制分析[J].中华中医药学刊,2023,41(1):69-73.[8]Zhao Q,Liu Y,Wang X,et al.Cuscuta chinensis flavonoids reducing oxidative stress of the improve sperm damage in bisphenol A exposed mice offspring[J].Ecotoxicol Environ Saf,2023,255:114831.[9]Chen Y,Ma L,Yan Y,et al.Ophiopogon japonicus polysaccharide reduces doxorubicin-induced myocardial ferroptosis injury by activating Nrf2/GPX4 signaling and alleviating iron accumulation[J].Mol Med Rep,2025,31(2):36.[10]Jiang Y,Wang L,Chen W,et al.Poria cocos polysaccharide prevents alcohol-induced hepatic injury and inflammation by repressing oxidative stress and gut leakiness[J].Front Nutr,2022,9:963598.[11]阳亚男.清热安胎膏治疗实验性大鼠妊娠期龈炎的疗效及对C反应蛋白的影响[D].长沙:湖南中医药大学,2023.[12]王楚睿,陈世娟,阳亚男,等.妊娠期龈炎前列腺素E2的表达及清热安胎膏干预作用初探[J].中医药临床杂志,2024,36(7):1340-1344.[13]郭德玉,叶翠飞,梁秀敏,等.大鼠实验性牙龈炎模型的制作及客观评定方法[J].实验动物科学,2011,28(4):63-65.[14]Ye C,You M,Huang P,et al.Clinical study showing a lower abundance of Neisseria in the oral microbiome aligns with low birth weight pregnancy outcomes[J].Clin Oral Investig,2022,26(3):2465-2478.[15]袁梦.妊娠期牙龈炎出现的原因及预防[J].造纸装备及材料,2020,49(1):254-255.[16]Ramji N,Xie S,Bunger A,et al.Effects of stannous fluoride dentifrice on gingival health and oxidative stress markers: a prospective clinical trial[J].BMC Oral Health,2024,24(1):1019.[17]郑如辉.碘甘油治疗孕妇急性智齿冠周炎的疗效观察[J].口腔材料器械杂志,2011,20(3):164.[18]LaFranchi SH.Thyroid Function in Preterm/Low Birth Weight Infants: Impact on Diagnosis and Management of Thyroid Dysfunction[J].Front Endocrinol (Lausanne),2021,12:666207.[19]王楚睿,陈世娟,阳亚男,等.妊娠期龈炎临床治疗方案研究概况[J].中医药临床杂志,2024,36(12):2431-2436.[20]李梦佳,王宏,董臣林,等.基于网络药理学探讨玉女煎治疗牙周炎的作用机制[J].中国医院用药评价与分析,2022,22(8):904-909.

更新日期/Last Update: 1900-01-01