[1]吴丽红,王悦宁,任丽英,等.基于代谢组学探究原花青素改善酒精肝机制[J].医学信息,2026,39(05):8-15.[doi:10.3969/j.issn.1006-1959.2026.05.002]
　WU Lihong,WANG Yuening,REN Liying,et al.Exploring the Mechanism of Procyanidin in Improving Alcoholic Liver Based on Metabolomics[J].Journal of Medical Information,2026,39(05):8-15.[doi:10.3969/j.issn.1006-1959.2026.05.002]
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基于代谢组学探究原花青素改善酒精肝机制()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
39卷
期数:
2026年05期
页码:
8-15
栏目:
生物信息学
出版日期:
2026-03-01

文章信息/Info

Title:
Exploring the Mechanism of Procyanidin in Improving Alcoholic Liver Based on Metabolomics
文章编号:
1006-1959（2026）05-0008-08
作者:
吴丽红; 王悦宁; 任丽英; 陈志刚
唐山市丰润区人民医院门诊药房，河北 唐山 064000
Author(s):
WU Lihong;  WANG Yuening;  REN Liying;  CHEN Zhigang
Clinical Pharmacy, Fengrun District People′s Hospital, Tangshan 064000, Hebei, China
关键词:
原花青素; 代谢组学; 酒精性肝损伤; 脂质代谢
Keywords:
Procyanidin;  Metabolomics;  Alcoholic liver damage;  Lipid metabolism
分类号:
R285.5
DOI:
10.3969/j.issn.1006-1959.2026.05.002
文献标志码:
A
摘要:
目的 探究原花青素B2（PB2）治疗酒精性肝损伤（ALD）的作用靶点及分子机制，为ALD的临床干预提供理论依据。方法 通过40%酒精溶液[10 ml/（kg·d）]灌胃建立小鼠ALD模型，设置正常对照组（NC组）、乙醇模型组（AL组）、水飞蓟宾阳性对照组（GL组）、高剂量PB2组（PBH组）和低剂量PB2组（PBL组），每组6只。检测血浆中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、甘油三酯（TG）、总胆固醇（TC）及酒精代谢相关标志物。利用LC-MS/MS技术进行血浆代谢组学分析，结合KEGG富集分析筛选PB2干预的关键代谢通路。结果 研究数据显示，PB2可显著降低ALD模型小鼠体内ALT、AST和TG水平（P＜0.05）。同时改善酒精代谢相关标志物，进而提高NAD+/NADH比值。代谢组学分析表明，PB2能够通过调节酒精中毒（alcoholism）、类固醇激素生物合成等代谢通路，改善ALD引发的脂质代谢紊乱问题。结论 PB2能显著降低ALD小鼠肝酶水平，改善酒精代谢标志物，调节相关代谢通路，为天然化合物干预 ALD 提供理论依据，也为相关药物研发提供新思路。
Abstract:
Objective To explore the target and molecular mechanism of procyanidin B2 (PB2) in the treatment of alcoholic liver damage (ALD), and to provide a theoretical basis for the clinical intervention of ALD. Methods The ALD model of mice was established by intragastric administration of 40% alcohol solution [10 ml/(kg·d)]. The normal control group (NC group), ethanol model group (AL group), silybin positive control group (GL group), high dose PB2 group (PBH group) and low dose PB2 group (PBL group) were set up, with 6 mice in each group. Alanine amino-transferase (ALT), aspartate transferase (AST), triglyceride (TG), total cholesterol (TC) and alcohol metabolism related markers in plasma were detected. LC-MS/MS technology was used for plasma metabolomics analysis, and KEGG enrichment analysis was used to screen the key metabolic pathways of PB2 intervention. Results The data showed that PB2 could significantly reduce the levels of ALT, AST and TG in ALD model mice (P<0.05). At the same time, the markers related to alcohol metabolism were improved, thereby increasing the NAD+/NADH ratio. Metabolomics analysis showed that PB2 could improve the lipid metabolism disorder caused by ALD by regulating metabolic pathways such as alcoholism and steroid hormone biosynthesis. Conclusion PB2 can significantly reduce the level of liver enzymes in ALD mice, improve alcohol metabolism markers, and regulate related metabolic pathways. It provides a theoretical basis for natural compounds to intervene in ALD, and also provides new ideas for related drug research and development.

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更新日期/Last Update: 1900-01-01