[1]吴 霞,肖盼盼,谢晓然,等.qRT-PCR法分析复发性SLE患者肠道双歧杆菌、 柔嫩梭菌数量的变化[J].医学信息,2018,31(05):65-69.[doi:10.3969/j.issn.1006-1959.2018.05.022]
　WU Xia,XIAO Pan-pan,XIE Xiao-ran,et al.Analysis of the Changes of Intestinal Bifidobacterium and Clostridium Tenella in Patients with Recurrent SLE by qRT-PCR[J].Journal of Medical Information,2018,31(05):65-69.[doi:10.3969/j.issn.1006-1959.2018.05.022]
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qRT-PCR法分析复发性SLE患者肠道双歧杆菌、 柔嫩梭菌数量的变化()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年05期
页码:
65-69
栏目:
论著
出版日期:
2018-03-01

文章信息/Info

Title:
Analysis of the Changes of Intestinal Bifidobacterium and Clostridium Tenella in Patients with Recurrent SLE by qRT-PCR
文章编号:
1006-1959（2018）05-0065-05
作者:
吴 霞1; 肖盼盼1; 谢晓然1; 孙雪茜2; 付冰冰2; 孙晓鹏2
1.佳木斯大学，黑龙江 佳木斯 154007；2.佳木斯大学附属第一医院风湿免疫科，黑龙江 佳木斯 154002
Author(s):
WU Xia1; XIAO Pan-pan1; XIE Xiao-ran1; SUN Xue-qian2; FU Bing-bing2; SUN Xiao-peng2
1.Jiamusi University,Jiamusi 154007,Heilongjiang,China; 2.Department of Rheumatological Immunology,First Affiliated Hospital of Jiamusi University,Jiamusi 154002,Heilongjiang,China
关键词:
系统性红斑狼疮; 双歧杆菌; 柔嫩梭菌; 实时荧光定量PCR
Keywords:
Key words：Systemic lupus erythematosus; Bifidobacterium; Clostridium tenella; Real-time fluorescence quantitative PCR
分类号:
R593.241
DOI:
10.3969/j.issn.1006-1959.2018.05.022
文献标志码:
A
摘要:
目的 运用实时荧光定量聚合酶链式反应（qRT-PCR）法分析系统性红斑狼疮患者肠道菌群数量的变化，探讨双歧杆菌、柔嫩梭菌与复发性SLE活动的关系。方法 提取粪便细菌组DNA，运用qRT-PCR法分析此二菌绝对数量的变化。结果 SLE复发活动期双歧杆菌、柔嫩梭菌数量均较治疗后稳定期减少（P＜0.05），对比正常对照组也均减少（P＜0.05）。结论 双歧杆菌、柔嫩梭菌菌群数量与SLE活动度评分相关，肠道内此二菌数量减少可能与SLE病情的复发有关。
Abstract:
Abstract：Objective To investigate the relationship between bifidobacterium,Clostridium tenella and recurrent SLE activity in patients with systemic lupus erythematosus by real-time quantitative polymerase chain reaction(qRT-PCR).Methods The DNA of fecal bacteria group was extracted,and the absolute quantity of the two bacteria was analyzed by qRT-PCR method.Results Bifidobacterium in the recurrent stage of SLE was found to be bifidobacterium.The number of Clostridium tenella was lower than that of stable period after treatment(P<0.05),compared with the normal control group,it was also decreased(P<0.05).Conclusion The number of bifidobacterium and Clostridium tenella was correlated with the SLE activity score,and the decrease of these two bacteria in the intestinal tract might be related to the recurrence of SLE.

参考文献/References:

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更新日期/Last Update: 2018-03-01