[1]户乃丽.流式细胞术研究细胞增殖的方法与技术[J].医学信息,2019,32(01):47-49.[doi:10.3969/j.issn.1006-1959.2019.01.016]
　HU Nai-Li.Method and Technique for Cell Proliferation by Flow Cytometry[J].Journal of Medical Information,2019,32(01):47-49.[doi:10.3969/j.issn.1006-1959.2019.01.016]
点击复制

流式细胞术研究细胞增殖的方法与技术()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年01期
页码:
47-49
栏目:
综述
出版日期:
2019-01-01

文章信息/Info

Title:
Method and Technique for Cell Proliferation by Flow Cytometry
文章编号:
1006-1959（2019）01-0047-03
作者:
户乃丽
首都医科大学中心实验室，北京 100069
Author(s):
HU Nai-Li
Central Laboratory,Capital Medical University,Beijing 100069,China
关键词:
细胞增殖; 流式细胞; DNA染色; 示踪染料
Keywords:
Cell proliferation; Flow cytometry; DNA staining; Tracer dye
分类号:
R392
DOI:
10.3969/j.issn.1006-1959.2019.01.016
文献标志码:
A
摘要:
流式细胞术是检测细胞增殖的一种重要手段，较以往的检测方法，流式细胞仪的优点在于能够实现多荧光指标同时检测，并且可以对混合样品中的特定细胞进行增殖分析，本文就目前常用细胞增殖检测染料的检测原理,应用范围及染色方法作进行阐述。
Abstract:
Flow cytometry is an important means to detect cell proliferation. Compared with previous detection methods, flow cytometry has the advantages of simultaneous detection of multiple fluorescence indicators, and can perform proliferation analysis on specific cells in mixed samples. At present, the detection principle, application range and dyeing method of cell proliferation detection dyes are commonly used.

参考文献/References:

[1]Zeng YT,Jiang JM,Lao HY,et al.Antitumor and apoptotic activities of the chemical constituents from the ethyl acetate extract of Artemisia indica[J].Molecular Medicine Reports,2015,11(3):2234-2240.
[2]Dapena C,Bravo I,Cuadrado A,et al.Nuclear and Cell Morphological Changes during the Cell Cycle and Growth of the Toxic Dinoflagellate Alexandrium minutum[J].Protist,2015,66(1):146-160.
[3]Uzlikova M,Nohynkova E.The effect of metronidazole on the cell cycle and DNA in metronidazole-susceptible and resistant Giardia cell lines[J].Molecular & Biochemical Parasitology,2014,198(2):75-81.
[4]柏春玲,廖泽容,张巧,等.应用流式细胞术快速测定细胞周期的DNA一步法[J].昆明医科大学学报,2017,38(2):10-13.
[5]Carbonari M.New use for an old reagent:Cell cycle analysis of DNA content using flow cytometry in formamide treated cells[J].Cytometry,2016,89(5):498-503.
[6]户乃丽,徐晓雪,邹林樾,等.利用405nm激光激发Hoechst33342染色细胞DNA的流式细胞技术[J].现代生物医学进展,2015,15(33):6406-6409.
[7]Buzina AR,Pinto FE,Nieschke K,et al.Replacement of specific markers for apoptosis and necrosis by nuclear morphology for affordable cytometry[J].Journal of Immunological Methods,2015,420(3):24-30.
[8]申雪沂,郭佳倩,牛长敏,等.Stra8过表达精原细胞的细胞周期同步化方法的建立[J].中国男科杂志,2017,31(6):3-7.
[9]Medina-Reyes EI,Bucio-López L,Freyre-Fonseca V,et al.Cell cycle synchronization reveals greater G2/M-phase accumulation of lung epithelial cells exposed to titanium dioxide nanoparticles[J].Environ Sci Pollut Res,2015,22(9):3976-3982.
[10]Tong J,Sun D,Yang C,et al.Serum starvation and thymidine double blocking achieved efficient cell cycle synchronization and altered the expression of p27, p53, bcl-2 in canine breast cancer cells[J]. Research in Veterinary Science,2016(4):10-14.
[11]邹林樾,徐晓雪,户乃丽.鸡红细胞标准品在胶质瘤细胞DNA周期检测中的意义[J].继续医学教育,2015,29(10):152-153.
[12]Massey AJ.Multiparametric Cell Cycle Analysis Using the Operetta High-Content Imager and Harmony Software with PhenoLOGIC[J].PLOS ONE,2015,10(7):e0134306.
[13]Zhao P,Fu JL,Yao BY,et al.S phase cell percentage normalized BrdU incorporation rate,a new parameter for determining S arrest[J].Biomed Environ Sci,2014,27(3):215-219.
[14]Harris L,Zalucki O,Piper M.BrdU/EdU dual labeling to determine the cell-cycle dynamics of defined cellular subpopulations[J].Journal of Molecular Histology,2018,49(3):229-234.
[15]Pereira PD,Serra-Caetano A,Cabrita M,et al.Quantification of cell cycle kinetics by EdU (5-ethynyl-2'-deoxyuridine)-coupled-fluorescence-intensity analysis[J].Oncotarget,2017,8(25):40514-40532.
[16]Bocharov G,Luzyanina T,Cupovic J,et al.Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis[J].Front Immunol,2013,4(9):264.
[17]Rodríguez-Pardo VM,Vernot JP.Mesenchymal stem cells promote a primitive phenotype CD34+ c-kit in human cord blood-derived hematopoietic stem cells during ex vivo expansion[J].Cell Mol Biol Lett,2013,18(1):11-33.
[18]杜振兰,陈鹏,封志纯.比较不同浓度CFSE荧光标记脐血细胞后存活率的实验研究[J].中华临床医师杂志,2016,10(3):368-372.
[19]Lyons AB,Blake SJ,Doherty KV.Flow Cytometric Analysis of Cell Division by Dilution of CFSE and Related Dyes[J].Current Protocols in Cytometry,2013(9):64.
[20]Luzyanina T,Cupovic J,Ludewig B,et al.Mathematical models for CFSE labelled lymphocyte dynamics:asymmetry and time-lag in division[J].Journal of Mathematical Biology,2014,69(6-7):1547-1583.

相似文献/References:

[1]周 业,张和良,刘重元,等.LMP1经Wnt1/β-catenin途径促进鼻咽癌干细胞SP18增殖[J].医学信息,2018,31(07):75.[doi:10.3969/j.issn.1006-1959.2018.07.024]
　ZHOU Ye,ZHANG He-liang,LIU Zhong-yuan,et al.LMP1 through Wnt1/β-catenin Pathway to Promote the Proliferation of SP18 in Nasopharyngeal Carcinoma Stem Cells[J].Journal of Medical Information,2018,31(01):75.[doi:10.3969/j.issn.1006-1959.2018.07.024]
[2]张新颖.芪参益气滴丸含药血清抑制血管平滑肌细胞增殖作用的实验研究[J].医学信息,2022,35(11):130.[doi:10.3969/j.issn.1006-1959.2022.11.034]
　ZHANG Xin-ying.Experimental Study on Inhibition of Vascular Smooth Muscle Cell Proliferation by Serum Containing Qishen Yiqi Dropping Pill[J].Journal of Medical Information,2022,35(01):130.[doi:10.3969/j.issn.1006-1959.2022.11.034]
[3]张天竺,宁 勇.长链非编码RNA调控乳腺癌发生发展的研究进展[J].医学信息,2019,32(12):40.[doi:10.3969/j.issn.1006-1959.2019.12.014]
　ZHANG Tian-zhu,NING Yong.Advances in Research on Long-chain Non-coding RNA Regulation of Breast Cancer Development[J].Journal of Medical Information,2019,32(01):40.[doi:10.3969/j.issn.1006-1959.2019.12.014]
[4]张 超,周 业,谢黎明,等.RTN4在胃癌发病机制中的作用[J].医学信息,2018,31(22):55.[doi:10.3969/j.issn.1006-1959.2018.22.016]
　ZHANG Chao,ZHOU Ye,XIE Li-min,et al.The Role of RTN4 in the Pathogenesis of Gastric Carcinoma[J].Journal of Medical Information,2018,31(01):55.[doi:10.3969/j.issn.1006-1959.2018.22.016]
[5]杨丽红,肖 波,侯丽霞,等.淫羊藿苷调节细胞增殖作用的研究进展[J].医学信息,2019,32(01):33.[doi:10.3969/j.issn.1006-1959.2019.01.012]
　YANG Li-hong,XIAO Bo,HOU Li-xia,et al.Advances in Research on the Regulation of Cell Proliferation by Icariin[J].Journal of Medical Information,2019,32(01):33.[doi:10.3969/j.issn.1006-1959.2019.01.012]
[6]李 莹,李小兵.人脂肪干细胞的生物学特性及分化研究[J].医学信息,2020,33(01):89.[doi:10.3969/j.issn.1006-1959.2020.01.027]
　LI Ying,LI Xiao-bing.Study on Biological Characteristics and Differentiation of Human Fat Stem Cells[J].Journal of Medical Information,2020,33(01):89.[doi:10.3969/j.issn.1006-1959.2020.01.027]
[7]张 莉,吴素琴,郭 毅,等.抗核抗体对人绒毛膜滋养层细胞系HTR8增殖及凋亡的影响[J].医学信息,2020,33(12):54.[doi:10.3969/j.issn.1006-1959.2020.12.017]
　ZHANG Li,WU Su-qin,GUO Yi,et al.Effects of Antinuclear Antibodies on the Proliferation and Apoptosis of Human Chorionic Trophoblast Cell Line HTR8[J].Journal of Medical Information,2020,33(01):54.[doi:10.3969/j.issn.1006-1959.2020.12.017]
[8]姜 玥,刘宵达,吴素琴.anti-IL-33对类风湿关节炎成纤维样滑膜细胞增殖与凋亡的影响[J].医学信息,2021,34(13):81.[doi:10.3969/j.issn.1006-1959.2021.13.020]
　JIANG Yue,LIU Xiao-da,WU Su-qin.The Effect of anti-IL-33 on the Proliferation and Apoptosis of Fibroblast-like Synovial Cells in Rheumatoid Arthritis[J].Journal of Medical Information,2021,34(01):81.[doi:10.3969/j.issn.1006-1959.2021.13.020]
[9]吕艳丽.联合高压氧治疗在创面修复中的应用研究[J].医学信息,2021,34(14):32.[doi:10.3969/j.issn.1006-1959.2021.14.009]
　LYU Yan-li.Study on the Application of Combined Hyperbaric Oxygen Therapy in Wound Healing[J].Journal of Medical Information,2021,34(01):32.[doi:10.3969/j.issn.1006-1959.2021.14.009]
[10]户乃丽,徐晓雪,邹林樾,等.FACSAriaⅢ流式细胞仪分选小鼠肺脏自然杀伤细胞方法的建立与评价[J].医学信息,2021,34(15):115.[doi:10.3969/j.issn.1006-1959.2021.15.030]
　HU Nai-li,XU Xiao-xue,ZOU Lin-yue,et al.Establishment and Evaluation of A Method for Sorting Mouse Lung Natural Killer Cells by FACSAria Ⅲ Flow Cytometer[J].Journal of Medical Information,2021,34(01):115.[doi:10.3969/j.issn.1006-1959.2021.15.030]

更新日期/Last Update: 2019-01-22