[1]刘 丽,孙立新,赵明珍,等.干扰素-γ对乳腺癌细胞HLA-Ⅰ分子表达的影响[J].医学信息,2019,32(04):88-90.[doi:10.3969/j.issn.1006-1959.2019.04.029]
　LIU Li,SUN Li-xin,ZHAO Ming-zhen,et al.Effect of Interferon-γ on HLA-Ⅰ Expression in Breast Cancer Cells[J].Journal of Medical Information,2019,32(04):88-90.[doi:10.3969/j.issn.1006-1959.2019.04.029]
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干扰素-γ对乳腺癌细胞HLA-Ⅰ分子表达的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年04期
页码:
88-90
栏目:
论著
出版日期:
2019-02-15

文章信息/Info

Title:
Effect of Interferon-γ on HLA-Ⅰ Expression in Breast Cancer Cells
文章编号:
1006-1959（2019）04-0088-03
作者:
刘 丽1; 孙立新2; 赵明珍2; 张晓威1
（1.承德医学院研究生院，河北 承德 067000；2.承德医学院附属医院中心实验室，河北 承德 067000）
Author(s):
LIU Li1; SUN Li-xin2; ZHAO Ming-zhen2; ZHANG Xiao-wei1
(1.Graduate School of Chengde Medical College,Chengde 067000,Hebei,China;2.Central Laboratory,Affiliated Hospital of Chengde Medical College,Chengde 067000,Hebei,China)
关键词:
干扰素-γ; 乳腺癌; HLA; 免疫治疗
Keywords:
Interferon-γ; Breast cancer; HLA; Immunotherapy
分类号:
R737.9
DOI:
10.3969/j.issn.1006-1959.2019.04.029
文献标志码:
A
摘要:
目的 研究干扰素-γ（IFN-γ）对乳腺癌细胞表面HLA-Ⅰ类分子表达的影响，并探讨其在乳腺癌治疗中的免疫作用机制。方法 培养乳腺癌SKBR3细胞株，根据IFN-γ浓度将其分为25 U/ml、50 U/ml、100 U/ml组，未加药组为空白对照组。通过实时荧光定量PCR和流式细胞术实验检测经不同浓度IFN-γ作用后的乳腺癌SKBR3细胞内HLA-Ⅰ mRNA的含量，以及表面的HLA-Ⅰ类抗原的表达。结果 实时荧光定量PCR结果显示，空白对照组、25 U/ml、50 U/ml、100 U/ml组HLA-Ⅰ mRNA的表达量分别为（1.00±0.00）、（2.26±0.54）、（3.89±0.81）、（5.94±0.97），三个浓度组均较空白对照组表达量高（P＜0.05）；流式细胞术结果显示，空白对照组、25 U/ml、50 U/ml、100 U/ml组细胞表面HLA-Ⅰ蛋白表达量为（14.87±2.80）、（44.33±3.36）、（48.80±2.63）、（56.77±1.93），三个浓度组均高于对照组（P＜0.05）。结论 IFN-γ上调乳腺癌细胞HLA-Ⅰ类抗原的表达有利于抗乳腺癌作用。
Abstract:
Objective To investigate the effect of interferon-γ (IFN-γ) on the expression of HLA class Ⅰ molecules on breast cancer cells and to explore its immune mechanism in the treatment of breast cancer. Methods Breast cancer SKBR3 cell line was cultured and divided into 25 U/ml,50 U/ml and 100 U/ml groups according to IFN-γ concentration. The untreated group was a blank control group. Real-time quantitative PCR and flow cytometry were used to detect the content of HLA-Ⅰ mRNA and the expression of HLA-Ⅰ antigen on the surface of breast cancer SKBR3 cells treated with different concentrations of IFN-γ. Results The real-time PCR results showed that the expression levels of HLA-Ⅰ mRNA in the blank control group, 25 U/ml, 50 U/ml and 100 U/ml were (1.00±0.00),(2.26±0.54),(3.89±0.81),(5.94±0.97),the three concentration groups were higher than the blank control group (P<0.05); the flow cytometry results showed that the blank control group, 25 U/ml, 50 U/ml The expression of HLA-Ⅰ protein in the cell surface of the 100 U/ml group was (14.87±2.80), (44.33±3.36), (48.80±2.63), (56.77±1.93), and the three concentration groups were higher than the control group (P<0.05). Conclusion IFN-γ up-regulates the expression of HLA-Ⅰ antigen in breast cancer cells, which is beneficial to the anti-breast cancer.

参考文献/References:


[1]Stewart B,Wild C.World Cancer Report 2014:International agency for research on cancer[M].Geneva:World Health Organization,2014.
[2]Siege RL,Miller KD,Jemaal A.Cancer statistics,2015[J].CA Cancer J Clin,2015,65(1):5-29.
[3]卢伸,苏丹.免疫检查点阻断用于肿瘤治疗的研究进展[J].实用肿瘤杂志,2016,31(1):19-23.
[4]吴明雨,于大海,程立,等.HLA-B在口腔鳞状细胞癌中的表达及意义[J].微生物学免疫学进展,2018,46(4):35-39.
[5]纪晓坤,王学利,王珩,等.Beclin1和HLAⅠ、Ⅱ在人卵巢癌SKOV3细胞中的表达[J].临床与实验病理学杂志,2017,33(9):954-958.
[6]Chang C,Campoli M,Ferrone S.Classical and nonclassical HLA class I antigen and NK cell-activating ligand changes in malignant cells: current challenges and future directions[J].Adv Cancer Res,2005(93):189-234．
[7]Abiko K,Matsumura N,Hamanishi J,et al.IFN-gamma from lymphocytes induces PD-L1 expression and promotes progression of ovarian cancer[J].Br J Cancer,2015,112(9):1501-1509.
[8]Zhang X,Zeng Y,Qu Q,et al.PD-L1 induced by IFN-gamma from tumor-associated macrophages via the JAK/STAT3 and PI3K/AKT signaling pathways promoted progression of lung cancer[J].Int J Clin Oncol,2017,22(6):1026-1033．
[9]李林卿,庄文欣,李鹏程,等.HLA-Ⅰ在乳腺癌组织中的表达及其临床意义[J].中国普外基础与临床杂志,2013,20(10):1132-1136.
[10]Kamali-Sarvestani E,Merat EA,Talei AR.Polymorphism in thegenes of alpha and beta tumor necrosis factors（TNF-alpha and TNF-beta）and gamma interferon（IFN-gamma）among Ira-nianwomen with breast cancer[J].Cancer Lett,2005,223(1):113-119.
[11]刘钢,黄文广,史火喜,等.结直肠癌人类白细胞抗原ABC表达与预后的相关性及干扰素γ的诱导作用研究[J].中华临床医师杂志(电子版),2009,3(7):1126-1134.
[12]Mimura K,Shiraishi K,Mueller A,et al.The MAPK pathway is a predominant regulator of HLA-A expression in esophageal and gastric cancer[J].J Immunol,2013(191):6261-6272.

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更新日期/Last Update: 2019-03-07