[1]王 建,胡立珍,张国翠,等.PNB/TCH生长试验和DNA微阵列基因芯片鉴定非结核分枝杆菌的价值比较[J].医学信息,2019,32(20):170-171.[doi:10.3969/j.issn.1006-1959.2019.20.054]
　WANG Jian,HU Li-zhen,ZHANG Guo-cui,et al.Comparison of PNB/TCH Growth and DNA Microarray Gene Chips for Identification of Nontuberculous Mycobacteria[J].Journal of Medical Information,2019,32(20):170-171.[doi:10.3969/j.issn.1006-1959.2019.20.054]
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PNB/TCH生长试验和DNA微阵列基因芯片鉴定非结核分枝杆菌的价值比较()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年20期
页码:
170-171
栏目:
诊疗技术
出版日期:
2019-10-15

文章信息/Info

Title:
Comparison of PNB/TCH Growth and DNA Microarray Gene Chips for Identification of Nontuberculous Mycobacteria
文章编号:
1006-1959（2019）20-0170-02
作者:
王 建1; 胡立珍1; 张国翠1; 赵 辉2; 陈子芳1
（山东省滨州市中心医院/滨州市结核病防治院检验科1，呼吸内科2，山东 滨州 251700）
Author(s):
WANG Jian1; HU Li-zhen1; ZHANG Guo-cui1; ZHAO Hui2; CHEN Zi-fang1
(Department of Clinical Laboratory1,Department of Respiratory Medicine2,Binzhou Central Hospital/Binzhou City Tuberculosis Hospital,Binzhou 251700,Shandong,China)
关键词:
非结核分枝杆菌; PNB/TCH; 基因芯片; 菌种鉴定
Keywords:
Non-tuberculous mycobacteria; PNB/TCH; Gene chip; Strain identification
分类号:
R440
DOI:
10.3969/j.issn.1006-1959.2019.20.054
文献标志码:
A
摘要:
目的 比较对硝基苯甲酸/噻吩-2-羧酸肼生长（PNB/TCH）和DNA微阵列基因芯片鉴定非结核分枝杆菌（NTM）临床分离株菌种的价值。方法 收集2013年1月~2018年6月我院住院患者的痰分枝杆菌培阳分离菌2175株，分别采用PNB/TCH生长试验和DNA微阵列基因芯片鉴别NTM，比较两种方法的鉴定结果。结果 PNB/TCH生长试验初步鉴定NTM 93株，占4.28%，DNA微阵列基因芯片鉴定NTM 79株，占3.63%，PNB/TCH生长试验初步鉴定的93株NTM中，有14例为假NTM（15.05%）；PNB/TCH生长试验鉴定NTM的阳性检出率低于DNA微阵列基因芯片；79株NTM中，占比前3位的依次为胞内分枝杆菌株（37.97%）、堪萨斯分枝杆菌（21.52%）、鸟分枝杆菌（16.46%）。结论 传统对硝基苯甲酸/噻吩-2-羧酸肼生长试验鉴定NTM特异性较低，采用DNA微阵列基因芯片技术鉴定NTM准确，临床参考价值较大。
Abstract:
Objective To compare the growth of non-tuberculous mycobacteria (NTM) clinical isolates with p-nitrobenzoic acid/thiophene-2-carboxylate growth (PNB/TCH) and DNA microarray microarray.Methods A total of 2175 strains of Mycobacterium phlei isolates in our hospital from January 2013 to June 2018 were collected. PNB/TCH growth test and DNA microarray gene chip were used to identify NTM. Identification of the method.Results PNB/TCH growth assay initially identified NTM 93 strain, accounting for 4.28%, DNA microarray gene chip identified NTM 79 strain, accounting for 3.63%, and of the 93 NTMs initially identified by PNB/TCH growth test, 14 were pseudo NTM ( 15.05%); PNB/TCH growth test identified that the positive detection rate of NTM was lower than that of DNA microarray gene chip; among the 79 strains of NTM, the top 3 were followed by intracellular branching strain (37.97%), Kansas Mycobacterium (21.52%), Mycobacterium avium (16.46%). Conclusion The traditional p-nitrobenzoic acid/thiophene-2-carboxylic acid oxime growth test has low specificity of NTM. The DNA microarray gene chip technology is used to identify NTM accurately, and the clinical reference value is large.

参考文献/References:

[1]全国第五次结核病抽样调查技术指导组,全国第五次结核病抽样调查办公室.2010年全国第五次结核病流行病学抽样调查报告[J].中国防痨杂志,2012,34(8):485-508. [2]赵雁林,逄宇.结核病实验室检验规程[M].北京:人民卫生出版社,2015. [3]刘海灿,黄明翔,蒋毅,等.福建省肺非结核分枝杆菌临床分离株的菌种鉴定[J].中国人兽共患病学报,2017,33(5):389-397. [4]柳正卫,吴蓓蓓,郑琳,等.微阵列芯片技术应用于分枝杆菌菌种鉴定的研究[J].浙江预防医学,2013,25(8):1-4. [5]余旭泉,徐礼锋,祝进,等.99株分枝杆菌菌种鉴定和耐药检测结果分析[J].中国卫生检验杂志,2015,25(20):3580-3582.

更新日期/Last Update: 2019-10-15