[1]侯柏龙,王 蔚,高雯洁.飞行时间质谱技术在肠炎沙门菌快速鉴定及同源性分析中的应用[J].医学信息,2021,34(09):176-178.[doi:10.3969/j.issn.1006-1959.2021.09.048]
　HOU Bai-long,WANG Wei,GAO Wen-jie.Application of Time-of-flight Mass Spectrometry in Rapid Identification and Homology Analysis of Salmonella Enteritidis[J].Medical Information,2021,34(09):176-178.[doi:10.3969/j.issn.1006-1959.2021.09.048]
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飞行时间质谱技术在肠炎沙门菌快速鉴定及同源性分析中的应用()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年09期
页码:
176-178
栏目:
诊疗技术
出版日期:
2021-05-01

文章信息/Info

Title:
Application of Time-of-flight Mass Spectrometry in Rapid Identification and Homology Analysis of Salmonella Enteritidis
文章编号:
1006-1959（2021）09-0176-03
作者:
侯柏龙; 王 蔚; 高雯洁
（1.嘉兴市第一医院检验科，浙江 嘉兴 314000； 2.嘉兴市疾病预防控制中心，浙江 嘉兴 314000）
Author(s):
HOU Bai-long; WANG Wei; GAO Wen-jie
(1.Clinical Laboratory,The First Hospital of Jiaxing City,Jiaxing 314000,Zhejiang,China; 2.Jiaxing Center for Disease Control and Prevention,Jiaxing 314000,Zhejiang,China)
关键词:
MALDI-TOF MS; PFGE; 肠炎沙门菌; 同源性分析
Keywords:
MALDI-TOF MS; PFGE; Salmonella enteritidis; Homology analysis
分类号:
R44
DOI:
10.3969/j.issn.1006-1959.2021.09.048
文献标志码:
A
摘要:
目的 研究基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）在肠炎沙门菌快速鉴定及同源性分析中的应用效果。方法 对2018年8月~12月嘉兴市第一医院患者大便标本分离出的11株肠炎沙门菌采用MALDI-TOF MS进行鉴定及同源性分析，并与VITEK-2 Compact仪鉴定结果和脉冲场凝胶电泳分型（PFGE）聚类分析结果进行比较。结果 收集得到的11株菌株经VITEK 2 Compact全自动微生物分析仪鉴定为沙门菌属历时约228 min，而MALDI-TOF MS鉴定只需8 min，差异有统计学意义（P＜0.05）。MALDI-TOF MS在聚类相似度为80%时，可以将11株肠炎沙门菌分为2型（MS1型和MS2型），其中MS1型为主要的质谱型，占90.91%。而PFGE在相似度＞95%时，也可以将11株肠炎沙门菌分为2型（E1型和E2型），两种方法分型结果相似度为73%，并不完全相同。结论 MALDI-TOF MS技术用于鉴定肠炎沙门菌，结果快速可靠，血清分型则需借助血清凝集试验。MALDI-TOF MS技术对肠炎沙门菌同源性分析结果与PFGE技术相似度为73%，可以作为肠炎沙门菌同源性分析方法的参考，为疾病防治以及疫情防控提供宝贵的时间差。
Abstract:
Objective To study the application effect of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in the rapid identification and homology analysis of Salmonella enteritidis.Methods The 11 strains of Salmonella enteritidis isolated from stool specimens of patients in the First Hospital of Jiaxing City from August to December 2018 were identified and analyzed by MALDI-TOF MS and compared with the identification results of VITEK-2 Compact instrument and the cluster analysis results of pulse field gel electrophoresis (PFGE).Results The collected 11 strains were identified as Salmonella by the VITEK 2 Compact automatic microbial analyzer, which lasted about 228 minutes, while the MALDI-TOF MS identification only took 8 min,the difference was statistically significant (P<0.05).When the cluster similarity of MALDI-TOF MS was 80%, 11 Salmonella enteritidis can be divided into 2 types (MS1 and MS2).Among them, MS1 was the main mass spectrometer, accounting for 90.91%. When PFGE had a similarity greater than 95%, 11 Salmonella enteritidis can also be divided into 2 types (E1 and E2).The similarity of the typing results of the two methods was 73%, which was not exactly the same.Conclusion MALDI-TOF MS technology is used to identify Salmonella enteritidis, and the results are fast and reliable. Serum agglutination test is needed for serotyping.The homology analysis results of MALDI-TOF MS technology for Salmonella enteritidis and PFGE technology are 73% similar, which can be used as a reference for the homology analysis method of Salmonella enteritidis, providing a valuable time difference for disease prevention and epidemic prevention and control.

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更新日期/Last Update: 1900-01-01