[1]柴仲秋,戴广法,周 冰.基于定量蛋白质组学的结直肠肿瘤脾气虚证患者差异表达蛋白研究[J].医学信息,2021,34(18):1-5.[doi:10.3969/j.issn.1006-1959.2021.18.001]
　CHAI Zhong-qiu,DAI Guang-fa,ZHOU Bing.Study on Differentially Expressed Proteins in Patients with Spleen Qi Deficiency Syndrome inColorectal Tumors Based on Quantitative Proteomics[J].Medical Information,2021,34(18):1-5.[doi:10.3969/j.issn.1006-1959.2021.18.001]
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基于定量蛋白质组学的结直肠肿瘤脾气虚证患者差异表达蛋白研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年18期
页码:
1-5
栏目:
出版日期:
2021-09-18

文章信息/Info

Title:
Study on Differentially Expressed Proteins in Patients with Spleen Qi Deficiency Syndrome inColorectal Tumors Based on Quantitative Proteomics
文章编号:
1006-1959（2021）18-0001-05
作者:
柴仲秋1; 戴广法2; 周 冰1
1.天津市滨海新区中医医院肛肠科，天津 300000；2.天津市滨海新区新港街社区卫生服务中心，天津 300000
Author(s):
CHAI Zhong-qiu1; DAI Guang-fa2; ZHOU Bing1
1.Department of Anorectal,Tianjin Binhai New Area Traditional Chinese Medicine Hospital,Tianjin 300000,China;2.Xingang Street Community Health Service Center,Binhai New District,Tianjin 300000,China
关键词:
结直肠肿瘤; 脾气虚证; 差异表达蛋白; 定量蛋白质组学
Keywords:
Colorectal neoplasms; Spleen-qi deficiency syndrome; Differentially expressed proteins; Quantitative proteomics
分类号:
R735.3
DOI:
10.3969/j.issn.1006-1959.2021.18.001
文献标志码:
A
摘要:
目的 探索结直肠肿瘤脾气虚证患者肿瘤组织与正常组织的差异表达蛋白，寻找该证型的中医治疗靶点。方法 选择2012年8月-2019年8月就诊于天津市滨海新区中医医院行手术治疗的结直肠肿瘤患者作为研究对象，对辨证为脾气虚证的患者留取术后肿瘤组织和远端正常组织标本，运用iTRAQ定量蛋白质组学比较两种组织的差异表达蛋白，并应用Metascape进行富集和分析。结果 脾气虚证患者肿瘤组织和远端正常组织表达差异的蛋白总数为140个，上调71个，下调69个；上下调差异倍数≥1.6倍的差异蛋白总数为42个，上调蛋白20个，下调蛋白22个；Metascape富集分析显示，差异蛋白的生物过程主要集中在平滑肌收缩、气体运输、粒细胞激活等通路中。结论 脾气虚证结直肠肿瘤患者肿瘤组织与正常组织间的蛋白表达存在差异，其差异蛋白的生物过程主要集中在平滑肌收缩、气体运输、粒细胞激活等通路中，这些通路对脾气虚证的结直肠肿瘤的形成具有重要作用。
Abstract:
Objective To explore the differentially expressed proteins between tumor tissues and normal tissues of patients with colorectal tumors with spleen-qi deficiency syndrome, and to find the target of TCM treatment for this syndrome.Methods Patients with colorectal tumors who underwent surgery at theTianjin Binhai New Area Traditional Chinese Medicine Hospital from August 2012 to August 2019 were selected as the research objects.The postoperative tumor tissue and distal normal tissue specimens were collected for patients with spleen-qi deficiency syndrome, and the differentially expressed proteins of the two tissues were compared using iTRAQ quantitative proteomics, and Metascape was used for enrichment and analysis.Results The total number of differentially expressed proteins in tumor tissue and distal normal tissues in patients with spleen-qi deficiency syndrome was 140, 71 were up-regulated and 69 were down-regulated;The total number of differential proteins with up- and down-regulated multiples of ≥ 1.6 times was 42, 20 up-regulated proteins, and 22 down-regulated proteins;Metascape enrichment analysis showed that the biological processes of differential proteins were mainly concentrated in smooth muscle contraction, gas transport, granulocyte activation and other pathways.Conclusion There are differences in protein expression between tumor tissues and normal tissues in colorectal tumor patients with spleen-qi deficiency syndrome.The biological processes of its differential proteins are mainly concentrated in smooth muscle contraction, gas transport, granulocyte activation and other pathways. These pathways play an important role in the formation of colorectal tumors with spleen-qi deficiency syndrome.

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更新日期/Last Update: 1900-01-01