[1]朱 茵,朱静怡,赵起超,等.细胞热转移技术的研究进展[J].医学信息,2022,35(03):29-32,48.[doi:10.3969/j.issn.1006-1959.2022.03.007]
 ZHU Yin,ZHU Jing-yi,ZHAO Qi-chao,et al.The Research Progress in Cellular Thermal Shift Assay[J].Medical Information,2022,35(03):29-32,48.[doi:10.3969/j.issn.1006-1959.2022.03.007]
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细胞热转移技术的研究进展()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年03期
页码:
29-32,48
栏目:
综述
出版日期:
2022-02-01

文章信息/Info

Title:
The Research Progress in Cellular Thermal Shift Assay
文章编号:
1006-1959(2022)03-0029-05
作者:
朱 茵1朱静怡1赵起超12谢 恬12陈功星12
1.杭州师范大学医学部,浙江 杭州 311121;2.浙产中药材资源开发与应用浙江省工程实验室,浙江 杭州 311121
Author(s):
ZHU Yin1ZHU Jing-yi1ZHAO Qi-chao12XIE Tian12CHEN Gong-xing12
1.Division of Health Sciences,Hangzhou Normal University,Hangzhou 311121,Zhejiang,China;2.Engineering Lab of Development and Application of Traditional Chinese Medicine from Zhejiang Province,Hangzhou 311121,Zhejiang,China
关键词:
细胞热转移技术蛋白质靶标热稳定
Keywords:
Cellular thermal shift assayProteinTargetThermal stability
分类号:
Q819
DOI:
10.3969/j.issn.1006-1959.2022.03.007
文献标志码:
A
摘要:
细胞热转移技术(CETSA)是一种利用蛋白质热稳定变化,可直接在细胞内检测靶标结合状况的方法,此技术已在新药研发、药物评估与优化、基因表达和疾病治疗等领域得到应用,随着不断地研究与应用逐步发展。CETSA与双向凝胶电泳(2-DE)、质谱分析法(MS)和荧光检测法等技术的结合,拓展了该技术的应用范围和价值。本文现对CETSA技术的基本原理、应用、发展情况进行综述及展望,旨在为该技术的在临床诊疗中的应用提供参考。
Abstract:
The cellular thermal shift assay (CETSA) can be used to detect directly the binding target with something in cells basing on the changes of the protein thermal stability. The assay have been applied in the new drug research and development, drug evaluation and optimization, gene expression and disease treatment, and has been gradually developed with continuous research and application. The combination of CETSA with two-dimensional gel electrophoresis(2-DE), mass spectrometry (MS) and fluorescence detection has expanded the application scope and value of this technology. This paper reviews the basic principle, application and development of CETSA technology, and looks forward to providing reference for its application in clinical diagnosis and treatment.

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更新日期/Last Update: 1900-01-01