[1]齐建军,杨麦青,张 林,等.TIPE2在透明细胞肾细胞癌中的表达和意义[J].医学信息,2023,36(04):82-87.[doi:10.3969/j.issn.1006-1959.2023.04.016]
 QI Jian-jun,YANG Mai-qing,ZHANG Lin,et al.The Expression and Significance of TIPE2 in Clear Cell Renal Carcinoma[J].Journal of Medical Information,2023,36(04):82-87.[doi:10.3969/j.issn.1006-1959.2023.04.016]
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TIPE2在透明细胞肾细胞癌中的表达和意义()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年04期
页码:
82-87
栏目:
论著
出版日期:
2023-02-15

文章信息/Info

Title:
The Expression and Significance of TIPE2 in Clear Cell Renal Carcinoma
文章编号:
1006-1959(2023)04-0082-06
作者:
齐建军杨麦青张 林
(1.昌邑市人民医院肾内科,山东 昌邑 261300;2.昌邑市人民医院病理科,山东 昌邑 261300;3.潍坊市人民医院/潍坊医学院第一附属医院病理科,山东 潍坊 261041;4.北大荒集团总医院病理科,黑龙江 哈尔滨 150088)
Author(s):
QI Jian-junYANG Mai-qingZHANG Linet al.
(1.Department of Nephrology,Changyi People’s Hospital,Changyi 261300,Shandong,China;2.Department of Pathology,Changyi People’s Hospital,Changyi 261300,Shandong,China;3.Department of Pathology,Weifang People’s Hospital/the First Affiliated Hospital of We
关键词:
TIPE2透明细胞肾细胞癌Wnt信号通路
Keywords:
TIPE2Clear cell renal cell carcinomaWnt signaling pathway
分类号:
R737.11
DOI:
10.3969/j.issn.1006-1959.2023.04.016
文献标志码:
A
摘要:
目的 观察TIPE2在透明细胞肾细胞癌(ccRCC)中的表达情况及与预后的关系,并探讨TIPE2在ccRCC中的作用机制。方法 利用UALCAN数据库分析TIPE2在ccRCC和正常肾组织中的表达差异,分析TIPE2表达与ccRCC临床病理参数之间的关系以及TIPE2表达评估ccRCC患者预后的价值;在ccRCC细胞786-O细胞系中,进行TIPE2质粒转染和TIPE2 shRNA干扰,Western Blot实验检测TIPE2在细胞内的转染和干扰效率,观察其对Wnt信号通路靶基因蛋白的影响,通过细胞增殖实验、集落形成实验和Transwell侵袭实验,观察不同TIPE2表达水平下786-O细胞的增殖和侵袭能力。结果 TIPE2 mRNA和蛋白水平在ccRCC组织中表达高于正常肾组织(P<0.05);TIPE2高表达提示ccRCC患者预后不良(P<0.05);在细胞学功能实验中,转染TIPE2可上调Wnt信号通路核心蛋白active-β-catenin以及Wnt靶基因如cyclin D1、c-Myc和MMP7的表达水平,并增强786-O细胞的增殖和侵袭能力(P<0.05);在干扰TIPE2后,active-β-catenin、cyclin D1、c-Myc和MMP7的表达水平减少,且786-O细胞的增殖和侵袭能力受到抑制(P<0.05)。结论 TIPE2在ccRCC中表达增高,并与ccRCC预后不良相关;TIPE2能够上调Wnt信号通路活性并促进ccRCC的增殖和侵袭能力。
Abstract:
Objective To observe the expression of TIPE2 in clear cell renal cell carcinoma (ccRCC) and its relationship with prognosis, and to explore the mechanism of TIPE2 in ccRCC.Methods The expression of TIPE2 in ccRCC and normal renal tissues was analyzed by UALCAN database. The relationship between TIPE2 expression and clinicopathological parameters of ccRCC and the value of TIPE2 expression in evaluating the prognosis of ccRCC patients were analyzed. TIPE2 plasmid transfection and TIPE2 shRNA interference were performed in ccRCC cell line 786-O.Western Blot assay was used to detect the transfection and interference efficiency of TIPE2 in cells, and its effect on Wnt signaling pathway target gene protein was observed. The proliferation and invasion ability of 786-O cells under different TIPE2 expression levels were observed by cell proliferation assay, colony formation assay and Transwell invasion assay.Results The expression of TIPE2 mRNA and protein in ccRCC tissues was higher than that in normal renal tissues (P<0.05). High expression of TIPE2 indicated poor prognosis in ccRCC patients (P<0.05). In the cytological function experiment, transfection of TIPE2 up-regulated the expression levels of Wnt signaling pathway core protein active-β-catenin and Wnt target genes such as cyclin D1, c-Myc and MMP7, and enhanced the proliferation and invasion of 786-O cells (P<0.05). After interference with TIPE2, the expression levels of active-β-catenin, cyclin D1, c-Myc and MMP7 were decreased, and the proliferation and invasion ability of 786-O cells were inhibited.Conclusion TIPE2 expression is increased in ccRCC and is associated with poor prognosis of ccRCC. TIPE2 can up-regulate the activity of Wnt signaling pathway and promote the proliferation and invasion of ccRCC.

参考文献/References:

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更新日期/Last Update: 1900-01-01