[1]刘家峰,胡金莲,张 颖,等.左归丸对慢性睡眠剥夺老年大鼠学习记忆及海马组织炎性因子的影响[J].医学信息,2023,36(06):73-76.[doi:10.3969/j.issn.1006-1959.2023.06.015]
　LIU Jia-feng,HU Jin-lian,ZHANG Ying,et al.Effects of Zuogui Pill on Learning and Memory and Inflammatory Factors in Hippocampus of Elderly Rats with Chronic Sleep Deprivation[J].Journal of Medical Information,2023,36(06):73-76.[doi:10.3969/j.issn.1006-1959.2023.06.015]
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左归丸对慢性睡眠剥夺老年大鼠学习记忆及海马组织炎性因子的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年06期
页码:
73-76
栏目:
论著
出版日期:
2023-03-15

文章信息/Info

Title:
Effects of Zuogui Pill on Learning and Memory and Inflammatory Factors in Hippocampus of Elderly Rats with Chronic Sleep Deprivation
文章编号:
1006-1959（2023）06-0073-04
作者:
刘家峰; 胡金莲; 张 颖; 等
（西安市第九医院中西医结合康复科1，神经内科2，神经外科3，陕西 西安 710054）
Author(s):
LIU Jia-feng; HU Jin-lian; ZHANG Ying; et al.
(Rehabilitation Department of Integrated Traditional Chinese and Western Medicine1,Department of Neurology2,Department of Neurosurgery3,Xi’an Ninth Hospital,Xi’an 710054,Shaanxi,China)
关键词:
慢性睡眠剥夺; 老年大鼠; 海马; 左归丸; 炎性因子; GDNF
Keywords:
Chronic sleep deprivation; Aged rats; Hippocampus; Zuogui pill; Inflammatory factor; GDNF
分类号:
R74
DOI:
10.3969/j.issn.1006-1959.2023.06.015
文献标志码:
A
摘要:
目的 观察左归丸对慢性睡眠剥夺老年大鼠学习记忆及海马炎性因子的影响，探讨其对慢性睡眠剥夺老年大鼠学习记忆作用机制的研究。方法 SPF级老年Wistar雄性大鼠40只，随机数字表法分成正常组、模型组、中药低剂量组、中药高剂量组、西药组，每组8只。正常组和模型组给等量生理盐水灌胃，中药低、中、高剂量组给予相应剂量的左归丸稀释液灌胃，西药组给予艾司唑仑片稀释液灌胃。采Morris水迷宫实验评价大鼠的记忆能力，ELISA法测定各组大鼠血清及海马炎性因子的表达。结果 与正常组比较，模型组大鼠在呼吸频率、心率、收缩压均增多，睡眠时间减低，穿越平台次数减少，逃避潜伏期时间延长，血清IL-1、IL-6水平升高，IL-10水平降低，脑组织GDNF水平降低（P＜0.05）；与模型组比较，中药低、高剂量组在呼吸频率、心率、收缩压均、睡眠时间均改善，穿越平台次数增多，逃避潜伏期时间减少，血清IL-1、IL-6水平降低，IL-10水平升高，脑组织GDNF水平升高（P＜0.05）；西药组大鼠在穿越平台次及逃避潜伏期时间无明显变化，血清IL-1、IL-6、IL-10无明显变化，脑组织GDNF无明显变化（P＞0.05）。结论 左归丸可改善老年大鼠水迷宫穿越平台次数及逃避潜伏期时长、改善睡眠时间，可能与其降低血清IL-1、IL-6，上调IL-10，进而改善大鼠脑组织中GDNF水平有关。
Abstract:
Objective To observe the effect of Zuogui pill on learning and memory and hippocampal inflammatory factors in elderly rats with chronic sleep deprivation, and to explore its mechanism of learning and memory in elderly rats with chronic sleep deprivation.Methods A total of 40 SPF aged Wistar male rats were randomly divided into normal group, model group, low-dose Chinese medicine group, high-dose Chinese medicine group and western medicine group, with 8 rats in each group. The normal group and the model group were given the same amount of normal saline by gavage. The low, medium and high dose groups of traditional Chinese medicine were given the corresponding dose of Zuogui pill diluted solution by gavage. The western medicine group was given estazolam tablets diluted solution by gavage. Morris water maze test was used to evaluate the memory ability of rats. The expression of inflammatory factors in serum and hippocampus of rats in each group was determined by ELISA.Results Compared with the normal group, the respiratory rate, heart rate and systolic blood pressure of the rats in the model group were significantly increased, the sleep time was significantly reduced, the number of crossing platforms was significantly reduced, the escape latency time was significantly prolonged, the levels of serum IL-1 and IL-6 were increased, the level of IL-10 was decreased, and the level of GDNF in brain tissue was decreased (P<0.05). Compared with the model group, the respiratory rate, heart rate, systolic blood pressure and sleep time of the low and high dose groups were significantly improved, the number of crossing the platform was significantly increased, the escape latency time was significantly reduced, the levels of serum IL-1 and IL-6 were decreased, the level of IL-10 was increased, and the level of GDNF in brain tissue was increased (P<0.05). In the western medicine group, there was no significant change in the time of crossing the platform and escape latency, serum IL-1, IL-6 and IL-10, and GDNF in brain tissue (P>0.05).Conclusion Zuogui pill can improve the times of crossing the platform and the escape latency in the water maze of elderly rats, and improve the sleep time, which may be related to the decrease of serum IL-1 and IL-6, the up-regulation of IL-10, and the improvement of GDNF level in brain tissue of rats.

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更新日期/Last Update: 1900-01-01