[1]余 亮,谭自明,张 斌,等.甘露糖结合凝集素基因多态性与结核病易感性关系的研究[J].医学信息,2018,31(13):65-67.[doi:10.3969/j.issn.1006-1959.2018.13.019]
　YU Liang,TAN Zi-ming,ZHANG Bin,et al.Relationship between Polymorphism of Mannose Binding Lectin Gene and Susceptibility to Tuberculosis[J].Journal of Medical Information,2018,31(13):65-67.[doi:10.3969/j.issn.1006-1959.2018.13.019]
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甘露糖结合凝集素基因多态性与结核病易感性关系的研究()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年13期
页码:
65-67
栏目:
论著
出版日期:
2018-07-01

文章信息/Info

Title:
Relationship between Polymorphism of Mannose Binding Lectin Gene and Susceptibility to Tuberculosis
文章编号:
1006-1959（2018）13-0065-03
作者:
余 亮; 谭自明; 张 斌; 赵 晶; 木哈达斯·吐尔逊依明; 李奇凤; 罗新辉; 孙 荷
新疆维吾尔自治区人民医院儿科研究所，新疆 乌鲁木齐 830001
Author(s):
YU Liang; TAN Zi-ming; ZHANG Bin; ZHAO Jing; Mukadas tursun emin; LI Qi-feng; LUO Xin-hui; SUN He
Institute of Pediatrics,the Xinjiang Uygur Autonomous Region People's Hospital,Urumqi 830001,Xinjiang,China
关键词:
甘露糖结合凝集素（MBL）; 结核病; 基因多态性; 新疆
Keywords:
Key words：Mannose binding lectin(MBL); Tuberculosis; Gene polymorphism; Xinjiang
分类号:
R521
DOI:
10.3969/j.issn.1006-1959.2018.13.019
文献标志码:
A
摘要:
目的 了解甘露糖结合凝集素基因多态性与新疆喀什地区结核病易感性的关系。方法 选取2015年12月~2017年12月在新疆喀什地区收集肺结核患者193例血液样本，收集同时期健康体检人员142例血液样本，采用试剂盒法提取全血基因组DNA，采用英国LGC公司生产的基于竞争性等位基因特异性PCR（KASP）技术的SNPline基因分型平台对选定的MBL基因SNP位点rs7096206进行分型，获得该位点的基因型频率和等位基因频率。结果 MBL基因rs7096206位点的三个基因型CC、GC、GG在结核患者组和对照组中的分布，差异无统计学意义（P＞0.05），但从等位基因在两组间的分布上看，突变型碱基G在结核患者组中的频率高于对照组，差异有统计学意义（P＜0.05）。结论 MBL基因rs7096206多态性位点与新疆结核病易感性存在关联，携带G碱基比携带C碱基具有更高的结核病发病风险。
Abstract:
Abstract：Objective To investigate the relationship between mannose-binding lectin gene polymorphism and susceptibility to tuberculosis in Kashgar,Xinjiang.Methods From December 2015 to December 2017,193 blood samples of pulmonary tuberculosis patients were collected in Kashgar,Xinjiang.142 blood samples were collected from concurrent health checkups.The whole blood genomic DNA was extracted by kit method,and the selected SNP locus rs7096206 was obtained by SNPline genotyping platform based on competitive allele-specific PCR(KASP)technology produced by LGC,UK.Genotypic frequency and allele frequency of this locus. Results There were no significant differences in the distribution of three genotypes CC,GC and GG of MBL gene rs7096206 in tuberculosis patients and control group(P>0.05),but the distribution of alleles between the two groups was observed.The frequency of mutant base G in the tuberculosis group was higher than that in the control group,and the difference was statistically significant(P<0.05).Conclusion The polymorphism of MBL gene rs7096206 is associated with susceptibility to tuberculosis in Xinjiang,and carrying G bases has a higher risk of tuberculosis than carrying C bases.

参考文献/References:

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更新日期/Last Update: 2018-07-01