[1]张 莉,吴素琴,郭 毅,等.抗核抗体对人绒毛膜滋养层细胞系HTR8增殖及凋亡的影响[J].医学信息,2020,33(12):54-57.[doi:10.3969/j.issn.1006-1959.2020.12.017]
　ZHANG Li,WU Su-qin,GUO Yi,et al.Effects of Antinuclear Antibodies on the Proliferation and Apoptosis of Human Chorionic Trophoblast Cell Line HTR8[J].Medical Information,2020,33(12):54-57.[doi:10.3969/j.issn.1006-1959.2020.12.017]
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抗核抗体对人绒毛膜滋养层细胞系HTR8增殖及凋亡的影响()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年12期
页码:
54-57
栏目:
论著
出版日期:
2020-06-15

文章信息/Info

Title:
Effects of Antinuclear Antibodies on the Proliferation and Apoptosis of Human Chorionic Trophoblast Cell Line HTR8
文章编号:
1006-1959（2020）12-0054-04
作者:
张 莉; 吴素琴; 郭 毅; 等
（1.沈阳市妇婴医院辅助生殖科，辽宁 沈阳 110014；2.辽宁省基础医学研究所免疫研究室，辽宁 沈阳 110101）
Author(s):
ZHANG Li; WU Su-qin; GUO Yi; et al
(1.Department of Assisted Reproduction,Shenyang Maternal and Child Hospital,Shenyang 110014,Liaoning,China;2.Department of Immunology,Liaoning Institute of Basic Medical Sciences,Shenyang 110101,Liaoning,China)
关键词:
抗核抗体; 滋养细胞; 细胞凋亡; 细胞增殖; 辅助生殖
Keywords:
Antinuclear antibody; Trophoblast; Apoptosis; Cell proliferation; Assisted reproduction
分类号:
R714.13
DOI:
10.3969/j.issn.1006-1959.2020.12.017
文献标志码:
A
摘要:
目的 研究抗核抗体对人绒毛膜滋养层细胞系HTR8增殖及凋亡的影响。方法 体外培养HTR8细胞，使用不同滴度的抗核抗体干预HTR8细胞，分为Control组、抗核抗体低浓度组（滴度1∶100）、抗核抗体高浓度组（滴度≥1∶320）。采用CCK-8法检测各组细胞增殖水平，流式细胞仪检测各组细胞凋亡，Western blot检测各组细胞bcl-2、bax蛋白表达水平。结果 抗核抗体高浓度组细胞增殖水平低于Control组，差异有统计学意义（P＜0.05）；抗核抗体低浓度组细胞增殖水平与Control组比较，差异无统计学意义（P＞0.05）；抗核抗体低浓度组和抗核抗体高浓度组细胞凋亡率均高于Control组，差异有统计学意义（P＜0.05）；抗核抗体低浓度组和抗核抗体高浓度组bax表达均高于Control组，bcl-2表达均低于Control组，差异有统计学意义（P＜0.05）。结论 抗核抗体通过抑制HTR8细胞增殖和促进其凋亡来抑制滋养细胞发育，其有可能影响辅助生殖的结局。
Abstract:
Objective To study the effect of antinuclear antibody on the proliferation and apoptosis of human chorionic trophoblast cell line HTR8. Methods HTR8 cells were cultured in vitro, and different titers of antinuclear antibodies were used to intervene in HTR8 cells. They were divided into Control group, antinuclear antibody low concentration group (titer 1:100), antinuclear antibody high concentration group (titer ≥1:320). The cell proliferation level of each group was detected by CCK-8 method, the apoptosis of each group was detected by flow cytometry, and the expression levels of bcl-2 and bax protein in each group were detected by Western blot.Results The cell proliferation level of the anti-nuclear antibody high concentration group was lower than that of the Control group,the difference was statistically significant (P<0.05); the cell proliferation level of the anti-nuclear antibody low concentration group was not statistically different from the Control group (P>0.05); Apoptosis was higher in the anti-nuclear antibody low concentration group and the anti-nuclear antibody high concentration group than in the Control group, the difference was statistically significant (P<0.05); bax expression was high in the anti-nuclear antibody low concentration group and the anti-nuclear antibody high concentration group in the Control group, the expression of bcl-2 was lower than that in the Control group,the difference was statistically significant (P<0.05). Conclusion Antinuclear antibodies inhibit the development of trophoblast cells by inhibiting the proliferation of HTR8 cells and promoting their apoptosis, which may affect the outcome of assisted reproduction.

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更新日期/Last Update: 1900-01-01