[1]毕凌波,尹 翠,张奉明,等.转基因敲除TRA1/TRPV1离子通道表达在慢性胰腺炎疼痛中的调控作用[J].医学信息,2020,33(15):65-68.[doi:10.3969/j.issn.1006-1959.2020.15.021]
　BI Ling-bo,YIN Cui,ZHANG Feng-ming,et al.The Regulatory Role of Transgenic Knockout TRA1/TRPV1 Ion Channel Expressionin Chronic Pancreatitis Pain[J].Medical Information,2020,33(15):65-68.[doi:10.3969/j.issn.1006-1959.2020.15.021]
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转基因敲除TRA1/TRPV1离子通道表达在慢性胰腺炎疼痛中的调控作用()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年15期
页码:
65-68
栏目:
论著
出版日期:
2020-08-01

文章信息/Info

Title:
The Regulatory Role of Transgenic Knockout TRA1/TRPV1 Ion Channel Expressionin Chronic Pancreatitis Pain
文章编号:
1006-1959（2020）15-0065-04
作者:
毕凌波1; 尹 翠1; 张奉明2; 顾佳慧1; 李 捷1; 祁小术1; 张笑婷1; 吴 丹1; 宗亚玲3
1.江苏省麻醉学重点实验室/江苏省麻醉与镇痛应用技术重点实验室，江苏 徐州 221004；2.江苏省人民医院麻醉科，江苏 南京 210000；3.山东第一医科大学第三附属医院药剂科，山东 济南 250031
Author(s):
BI Ling-bo1; YIN Cui1; ZHANG Feng-ming2; GU Jia-hui1; LI Jie1; QI Xiao-shu1; ZHANG Xiao-ting1; WU Dan1; ZONG Ya-ling3
1.Jiangsu Provincial Key Laboratory of Anesthesiology/Jiangsu Provincial Key Laboratory of Anesthesia and Analgesia Applied Technology,Xuzhou 221004,Jiangsu,China;2.Department of Anesthesiology,Jiangsu Provincial People’s Hospital,Nanjing 210000,Jiangsu
关键词:
TRPA1; TRPV1; 慢性胰腺炎; 疼痛
Keywords:
TRPA1; TRPV1; Chronic pancreatitis; Pain
分类号:
R657.5+1
DOI:
10.3969/j.issn.1006-1959.2020.15.021
文献标志码:
A
摘要:
目的 观察TRPA1/TRPV1通道对慢性胰腺炎疼痛的调控作用。方法 选取C57野生型小鼠14只，采用随机数字表法分为空白对照组（WT-Vehicle组）和慢性胰腺炎模型组（WT-DBTC组），每组7只。另选实验室培育繁殖的C57转基因小鼠，根据基因鉴定结果，选取TRPA1基因敲除组（TRPA1-KO-DBTC组）7只和TRPV1基因敲除组（TRPV1-KO-DBTC组）10只，未造模时测定四组小鼠腹部机械痛阈和热痛阈基础值，分别在第1、7、14天经口灌胃方式对WT-DBTC组、TRPA1-KO-DBTC组和TRPV1-KO-DBTC组按体重给予8 mg/kg二丁基二氯化锡（DBTC）进行造模，每日监控体重变化。造模结束后第7天再次测定四组小鼠腹部机械痛阈和热痛阈，比较造模前和造模后各组小鼠机械痛阈和热痛阈。结果 造模结束7天后，WT-DBTC组小鼠的腹部机械痛阈和热痛阈低于WT-Vehicle组（P＜0.05）；而TRPA1-KO-DBTC组和TRPV1-KO-DBTC组小鼠的腹部机械痛阈和热痛阈高于WT-DBTC组小鼠（P＜0.05）。结论 全身敲除TRPA1和TRPV1通道可以抑制慢性胰腺炎诱发的机械痛敏和热痛敏，TRPA1和TRPV1通道在慢性胰腺炎疼痛中发挥着重要作用。
Abstract:
Objective To observe the regulation effect of TRPA1/TRPV1 channel on chronic pancreatitis pain.Methods Fourteen C57 wild-type mice were selected and divided into a blank control group (WT-Vehicle group) and a chronic pancreatitis model group (WT-DBTC group) using a random number table method, with 7 mice in each group. Another selected laboratory to breed C57 transgenic mice, according to the results of gene identification, select 7 TRPA1 knockout group (TRPA1-KO-DBTC group) and 10 TRPV1 knockout group (TRPV1-KO-DBTC group). The basic values of abdominal mechanical pain threshold and thermal pain threshold were measured in four groups without modeling. On day 1,7 and 14, WT-DBTC group, TRPA1-KO-DBTC group and TRPV1-KO-DBTC group were given 8 mg/kg dibutyltin dichloride (DBTC) according to body weight to monitor body weight changes daily. On the 7th day after modeling, the mechanical pain threshold and thermal pain threshold of the four groups of mice were measured again, and the mechanical pain threshold and thermal pain threshold of each group were compared before and after modeling. Results 7 days after modeling, the abdominal mechanical pain threshold and thermal pain threshold of the WT-DBTC group were lower than those of the WT-Vehicle group (P＜0.05); while the TRPA1-KO-DBTC group and TRPV1-KO-DBTC group mice the abdominal mechanical pain threshold and thermal pain threshold were higher than those in the WT-DBTC group (P＜0.05).Conclusion Systemic knockout of TRPA1 and TRPV1 channels can inhibit the mechanical and thermal hyperalgesia induced by chronic pancreatitis. TRPA1 and TRPV1 channels play an important role in chronic pancreatitis pain.

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更新日期/Last Update: 1900-01-01