[1]罗纯生,展秀君.化学发光免疫分析法与酶联免疫吸附试验法检测乙肝病毒感染标志物比较[J].医学信息,2022,35(09):175-177.[doi:10.3969/j.issn.1006-1959.2022.09.045]
　LUO Chun-sheng,ZHAN Xiu-jun.Comparison of Chemiluminescence Immunoassay and Enzyme-linked Immunosorbent Assay for Detection of HBV Infection Markers[J].Medical Information,2022,35(09):175-177.[doi:10.3969/j.issn.1006-1959.2022.09.045]
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化学发光免疫分析法与酶联免疫吸附试验法检测乙肝病毒感染标志物比较()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年09期
页码:
175-177
栏目:
诊疗技术
出版日期:
2022-05-01

文章信息/Info

Title:
Comparison of Chemiluminescence Immunoassay and Enzyme-linked Immunosorbent Assay for Detection of HBV Infection Markers
文章编号:
1006-1959（2022）09-0175-03
作者:
罗纯生; 展秀君
（1.佳木斯市中心血站，黑龙江 佳木斯 154002；2.佳木斯市中医医院，黑龙江 佳木斯 154002）
Author(s):
LUO Chun-sheng; ZHAN Xiu-jun
(1.Jiamusi Central Blood Station,Jiamusi 154002,Heilongjiang,China;2.Jiamusi Hospital of Traditional Chinese Medicine,Jiamusi 154002,Heilongjiang,China)
关键词:
化学发光免疫分析法; 酶联免疫吸附试验法; 乙肝病毒; 感染标志物
Keywords:
Chemiluminescence immunoassay; Enzyme linked immunosorbent assay; Hepatitis B virus; Infection markers
分类号:
R446.6
DOI:
10.3969/j.issn.1006-1959.2022.09.045
文献标志码:
A
摘要:
目的 比较化学发光免疫分析法与酶联免疫吸附试验法检测乙肝病毒感染标志物的价值。方法 选取2020年1月-2021年5月在我站血液采集筛查，经实时荧光定量PCR确诊的70例乙肝病毒阳性样本为研究对象，采用随机数字表法分为对照组和观察组，各35例。对照组采用酶联免疫吸附试验法检测，观察组采用化学发光免疫分析法检测，比较两组乙肝病毒感染标志物检测阳性率、两组乙肝病毒感染灵敏度、特异度、准确度以及低浓度HBsAg诊断准确度。结果 观察组乙型肝炎E抗体（HBeAb）、乙型肝炎表面抗原（HBsAg）、乙型肝炎E抗原（HBeAg）阳性检出率均高于对照组，差异有统计学意义（P＜0.05）；两组乙型肝炎表面抗体（HBsAb）、乙型肝炎核心抗体（HBcAb）阳性检出率比较，差异无统计学意义（P＞0.05）；两组乙肝病毒感染标志物灵敏度、特异度、准确度比较，差异无统计学意义（P＞0.05）；当HBsAg低水平检测值≤1.00 ng/ml时，观察组乙肝病毒感染标志物检出率高于对照组，差异有统计学意义（P＜0.05）。结论 化学发光免疫分析法与酶联免疫吸附试验法在乙肝病毒感染标志物检测中均具有良好灵敏度、特异度、准确度，但是化学发光免疫分析法对乙肝病毒感染标志物检出阳性率相对较高，尤其对于早期乙肝病毒感染具有重要的临床应用价值。
Abstract:
Objective To compare the value of chemiluminescence immunoassay and enzyme linked immunosorbent assay in detection of hepatitis B virus infection markers.Methods A total of 70 patients with hepatitis B virus positive samples confirmed by real-time fluorescence quantitative PCR were selected from January 2020 to May 2021 in our station. They were divided into control group and observation group by random number table method, with 35 cases in each group. The control group was detected by enzyme-linked immunosorbent assay, and the observation group was detected by chemiluminescence immunoassay. The positive rate of hepatitis B virus infection markers, the sensitivity, specificity, accuracy of hepatitis B virus infection and the diagnostic accuracy of low concentration HBsAg in the two groups were compared.Results The positive detection rates of hepatitis B E antibody (HBeAb), hepatitis B surface antigen (HBsAg) and hepatitis B E antigen (HBeAg) in the observation group were higher than those in the control group, and the difference was statistically significant (P<0.05). There was no significant difference in the positive detection rates of hepatitis B surface antibody (HBsAb) and hepatitis B core antibody (HBcAb) between the two groups (P>0.05). There was no significant difference in sensitivity, specificity and accuracy of hepatitis B virus infection markers between the two groups (P>0.05). When the low-level detection value of HBsAg was ≤1.00 ng/ml, the detection rate of HBV infection markers in the observation group was higher than that in the control group, and the difference was statistically significant (P<0.05).Conclusion Chemiluminescence immunoassay and enzyme-linked immunosorbent assay have good sensitivity, specificity and accuracy in the detection of hepatitis B virus infection markers, but the positive rate of chemiluminescence immunoassay for hepatitis B virus infection markers is relatively high, especially for early hepatitis B virus infection.

参考文献/References:

[1]赵棉,张力,赵亚妮.HBVDNA定量与乙肝血清学标志物定量联合检测乙肝病毒感染的效果分析[J].实用临床医药杂志,2018,22(15):37-40.[2]王新莉.酶联免疫吸附试验与化学发光免疫分析法检测乙型肝炎5项血清标志物的对比分析[J].国际检验医学杂志,2016,37(6):807-808.[3]李林臣,张懿晖,牛艳芬,等.全自动化学发光分析仪及核酸扩增仪检测乙肝标志物的应用[J].中国医学装备,2016,13(4):41-44.[4]杨梅,晏红.化学发光免疫分析法检测乙肝病毒感染性标志物的临床应用[J].标记免疫分析与临床,2016,23(4):448-450.[5]张秀莲,江峰.不同免疫检验方法检测乙肝病毒感染血清标志物的对比观察及对HBsAg、HBeAb的影响[J].临床合理用药杂志,2018,11(35):135-136.[6]段海萍,王侠.化学发光酶免疫分析法在乙肝病毒及核心抗体定性检测中的应用分析[J].国际病毒学杂志,2016,23(1):57-59,63.[7]殷蓓琦,黄秋芳.三种不同免疫检测法对乙肝血清学标志物检测的结果对比分析[J].标记免疫分析与临床,2017,24(6):682-685.[8]周丹,何进才,李惠贞,等.荧光定量PCR仪检测乙型肝炎病毒核酸的性能验证及评价[J].检验医学与临床,2019,16(01):80-84.[9]朱为刚,曾劲峰,李彤,等.一种化学发光检测试剂在血液筛查中的应用评估[J].中国输血杂志,2016,29(6):581-583.[10]李燕,蔡利超.化学发光酶免疫分析法在乙肝病毒及核心抗体定性检测中的应用价值[J].实用中西医结合临床,2017,17(6):97-98.[11]池妤,曹谊.乙肝肝炎表面抗原阴性献血者HBVDNA与乙肝二对半检测结果分析[J].中国基层医药,2017,24(13):2068-2069.[12]许丽枫,杨建宝,马媛媛,等.化学发光微粒子免疫检测法检验3172例乙型肝炎五项的结果模式分析[J].山西医药杂志,2016,45(2):211-212.[13]熊海燕,罗业飞,刘海燕,等.酶联免疫吸附试验和电化学发光免疫法检测孕妇乙型肝炎表面抗原结果的比较[J].中华流行病学杂志,2017,38(11):1537-1540.[14]李欣,李万顺,王海宁.酶联免疫吸附试验、胶体金法和化学发光法检测孕妇人巨细胞病毒感染的对比研究[J].陕西医学杂志,2016,45(10):1390-1391.[15]何海艳,张国平,魏兆飞,等天津市慢性乙肝社会负担指标构建及其权重系数的初步研究[J].中国慢性病预防与控制,2020,28(8):596-599.[16]李仁杰,徐灵,张宇亮,等.乙型肝炎患者病毒复制指标与乙肝标志物及肝功能关系分析[J].临床和实验医学杂志,2017,16(3):225-227.[17]李锋,王菊英,姚琳芳,等.应用ROC曲线评价化学发光法及酶联免疫法对HCV感染的诊断价值[J].国际病毒学杂志,2016,23(6):398-400,404.[18]韩玮玮,金齐力,刘嘉清,等.六种国产乙型肝炎表面抗体ELISA试剂盒符合率及cut-off值探讨[J].安徽医科大学学报,2020,55(4):621-628.[19]门守山,商发奎,韩春花,等.化学发光法与酶联免疫吸附试验检测献血者梅毒螺旋体特异性抗体结果分析[J].中国实验血液学杂志,2017,25(1):226-230.[20]张守彩,高玉,郑桂喜,等.两种方法评估胃蛋白酶原在萎缩性胃炎和胃癌中的诊断价值[J].山东大学学报(医学版),2016,54(12):46-52.

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更新日期/Last Update: 1900-01-01