[1]刘亮亮,文 华,杨 瑾,等.TLR4/NF-κB通路在慢性鼻-鼻窦炎伴鼻息肉和不伴鼻息肉的黏膜上皮修复机制中的作用[J].医学信息官方网站,2022,35(15):39-45.[doi:10.3969/j.issn.1006-1959.2022.15.008]
　LIU Liang-liang,WEN Hua,YANG Jin,et al.The Role of TLR4/NF-κB Signaling Pathway in the Repair of Mucosal Epithelium in Chronic Sinusitis with Nasal Polyps and Without Nasal Polyps[J].Medical Information,2022,35(15):39-45.[doi:10.3969/j.issn.1006-1959.2022.15.008]
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TLR4/NF-κB通路在慢性鼻-鼻窦炎伴鼻息肉和不伴鼻息肉的黏膜上皮修复机制中的作用()
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《医学信息》官方网站[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年15期
页码:
39-45
栏目:
论著
出版日期:
2022-08-01

文章信息/Info

Title:
The Role of TLR4/NF-κB Signaling Pathway in the Repair of Mucosal Epithelium in Chronic Sinusitis with Nasal Polyps and Without Nasal Polyps
文章编号:
1006-1959（2022）15-0039-07
作者:
刘亮亮; 文 华; 杨 瑾; 等
（1.西安交通大学医院眼耳鼻咽喉科，陕西 西安 710049；2.西安交通大学医院公共卫生中心，陕西 西安 710049；3.西安交通大学医院妇产科，陕西 西安 710049；4.西安交通大学第二附属医院耳鼻咽喉头颈外科病院，陕西 西安 710004）
Author(s):
LIU Liang-liang; WEN Hua; YANG Jin; et al.
(1.Department of Ophthalmology,Otolaryngology,Xi’an Jiaotong University Hospital,Xi’an 710049,Shaanxi,China;2.Public Health Center of Xi’an Jiaotong University Hospital,Xi’an 710049,Shaanxi,China;3.Department of Obstetrics and Gynecology,Xi’an Jiaotong University Hospital,Xi’an 710049,Shaanxi,China;4.Department of Otolaryngology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,Shaanxi,China)
关键词:
慢性鼻-鼻窦炎伴鼻息肉; 慢性鼻-鼻窦炎不伴鼻息肉; 黏膜上皮; TLR4/NF-κB信号通路; 修复
Keywords:
Chronic rhinosinusitis with nasal polyps; Chronic rhinosinusitis without nasal polyps; Mucosal epithelium; TLR4/NF-κB signal pathway; Repair
分类号:
R765
DOI:
10.3969/j.issn.1006-1959.2022.15.008
文献标志码:
A
摘要:
目的 探讨TLR4/NF-κB通路在慢性鼻-鼻窦炎（CRS）伴鼻息肉（CRSwNP）和不伴鼻息肉（CRSsNP）的黏膜上皮修复机制中的作用。方法 收集2018年3月-2021年9月就诊于西安交通大学附属医院耳鼻咽喉科的30例CRS患者的鼻筛窦黏膜上皮组织。将CRS患者组织分为CRSsNP组和CRSwNP组，每组10例。对照组组织来源于进行视觉性鼻整形术的患者。HE染色、免疫组化法检测各组患者鼻黏膜组织病理学改变以及TLR4、NF-κB的表达；将各组鼻粘膜上皮细胞进行离体培养，分别以4种因素（空白对照组；EGF组：添加50 ml 5 ng/ml EGF；TLR4/NF-κB组：依次添加5 ng/ml TLR4和NF-κB培养24 h后，添加5 ng/ml EGF；PDTC组：添加5 ng/ml信号通路抑制剂PDTC培养12 h后，添加5 ng/ml TLR4和NF-κB培养12 h后，添加5 ng/ml EGF）刺激各组鼻粘膜上皮细胞，CCK-8法、ELISA检测各组鼻黏膜上皮细胞的增殖以及IL-β、1L-5的表达。结果 与CRSsNP组相比，CRSwNP组的鼻窦CT评分明显升高（P＜0.05）；与对照组患者相比，CRSsNP组、CRSwNP组TLR4、NF-κB的表达量升高（P＜0.05）；与CRSsNP组相比，CRSwNP组TLR4、NF-κB的表达量升高（P＜0.05）；CCK-8和ELISA法实验结果：与对照组相比，CRS组鼻粘膜上皮细胞的增殖基线降低，IL-β、IL-5的水平升高（P＜0.05）；与CRSsNP组相比，CRSwNP组鼻粘膜上皮细胞的增殖基线升高，IL-β、IL-5的水平升高（P＜0.05）；对照组和CRS组加入EGF后，与空白对照相比，鼻粘膜上皮细胞的增殖基线明显升高，IL-β、IL-5的水平降低（P＜0.05）；加入TLR4和NF-κB后，与空白对照相比，鼻粘膜上皮细胞的增殖基线降低，IL-β、IL-5的水平升高（P＜0.05）；加入PDTC后，EGF诱导细胞增值的作用明显有所恢复，IL-β、IL-5的水平明显有所降低（P＜0.05）。结论 TLR4/NF-κB信号通路影响CRS中CRSsNP和CRSwNP两者鼻粘膜上皮细胞损伤后的修复，抑制TLR4/NF-κB信号通路能明显增强CRS患者鼻粘膜上皮细胞的增殖作用。
Abstract:
Objective To investigate the role of TLR4/NF- κB pathway in the repair of mucosal epithelium in chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP)and chronic rhinosinusitis without nasal polyps (CRSsNP).Methods The nasal ethmoid sinus mucosal epithelial tissues of 30 patients with CRS were collected from the Department of Otolaryngology, Affiliated Hospital of Xi ’ an Jiaotong University from March 2018 to September 2021. The tissues of CRS patients were divided into CRSsNP group and CRSwNP group, with 10 cases in each group. The control group was derived from patients undergoing visual rhinoplasty. HE staining and immunohistochemistry were used to detect the pathological changes of nasal mucosa and the expressions of TLR4 and NF-κB in each group. The nasal epithelial cells of each group were cultured in vitro with four factors (blank control group; eGF group: adding 50 ml 5 ng/ml EGF; TLR4/NF-κB group: 5 ng/ml EGF was added after TLR4 and NF-κB were cultured for 24 h; PDTC group: After PDTC with 5 ng/ml signal pathway inhibitor was added for 12 h, TLR4 with 5 ng/ml and NF-κB were added for 12 h, and EGF with 5 ng/ml was added to stimulate nasal epithelial cells in each group. CCK-8 method and ELISA were used to detect the proliferation of nasal epithelial cells and the expression of IL-β and IL-5 in each group.Results Compared with CRSsNP group, the CT score of sinus in CRSwNP group was significantly increased (P<0.05). Compared with the control group, the expression of TLR4 and NF-κB in CRSsNP group and CRSwNP group increased (P<0.05). Compared with CRSsNP group, the expression of TLR4 and NF-κB in CRSwNP group increased (P<0.05). CCK-8 and ELISA results showed that compared with the control group, the proliferation baseline of nasal epithelial cells in CRS group was decreased, and the levels of IL-β and IL-5 were increased (P<0.05). Compared with CRSsNP group, the proliferation baseline of nasal epithelial cells in CRSwNP group was increased, and the levels of IL-β and IL-5 were increased (P<0.05). After EGF was added to the control group and CRS group, compared with the blank control group, the proliferation baseline of nasal epithelial cells was significantly increased, and the levels of IL-β and IL-5 were decreased (P<0.05). After TLR4 and NF-κB were added, compared with the blank control, the proliferation baseline of nasal epithelial cells was decreased, and the levels of IL-β and IL-5 were increased (P<0.05). After adding PDTC, the effect of EGF on cell proliferation was significantly restored, and the levels of IL-β and IL-5 were significantly decreased (P<0.05).Conclusion TLR4/NF-κB signaling pathway affects the repair of nasal epithelial cells damaged by CRSsNP and CRSwNP in CRS. Inhibition of TLR4/NF-κB signaling pathway can significantly enhance the proliferation of nasal epithelial cells in CRS patients.

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更新日期/Last Update: 1900-01-01