[1]马若飞,苏 苗,徐韦玮,等.归芪益元膏对气血两虚证模型大鼠的药效学评价[J].医学信息,2023,36(16):72-77.[doi:10.3969/j.issn.1006-1959.2023.16.013]
　MA Ruo-fei,SU Miao,XU Wei-wei,et al.Pharmacodynamics Evaluation of Guiqi Yiyuan Ointment on Model Rats with Deficiency of Both Qi and Blood[J].Journal of Medical Information,2023,36(16):72-77.[doi:10.3969/j.issn.1006-1959.2023.16.013]
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归芪益元膏对气血两虚证模型大鼠的药效学评价()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年16期
页码:
72-77
栏目:
论著
出版日期:
2023-08-15

文章信息/Info

Title:
Pharmacodynamics Evaluation of Guiqi Yiyuan Ointment on Model Rats with Deficiency of Both Qi and Blood
文章编号:
1006-1959（2023）16-0072-06
作者:
马若飞; 苏 苗; 徐韦玮; 等
（1.甘肃中医药大学中医临床学院，甘肃 兰州 730101；2.敦煌医学与转化教育部重点实验室，甘肃 兰州 730000；3.甘肃中医药大学基础医学院，甘肃 兰州 730101；4.甘肃中医药大学药学院，甘肃 兰州 730101；5.甘肃中医药大学科研实验中心，甘肃 兰州 730000）
Author(s):
MA Ruo-fei; SU Miao; XU Wei-wei; et al.
(1.Clinical College of Traditional Chinese Medicine,Gansu University of Chinese Medicine,Lanzhou 730101,Gansu,China; 2.Key Laboratory of Dunhuang Medicine and Transformation,Ministry of Education,Lanzhou 730000,Gansu,China; 3.School of Basic Medicine,Gansu University of Chinese Medicine,Lanzhou 730101,Gansu,China;4.School of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730101,Gansu,China; 5.Scientific Research and Experiment Center,Gansu University of Chinese Medicine,Lanzhou 730101,Gansu,China)
关键词:
归芪益元膏; 气血两虚证; 药效学; 力竭游泳时间
Keywords:
Guiqi Yiyuan ointment; Deficiency of both qi and blood; Pharmacodynamics; Exhaustive swimming time
分类号:
R285.5
DOI:
10.3969/j.issn.1006-1959.2023.16.013
文献标志码:
A
摘要:
目的 探讨归芪益元膏对气血两虚证模型大鼠的药效学作用。方法 以“限制饮食+负重游泳”和注射乙酰苯肼建立气血两虚大鼠模型，采用边造模边给药的方法，给予归芪益元膏进行灌胃干预。观察大鼠耳缘、鼻唇颜色，精神状态等证候学指标，并测定力竭游泳时间；腹主动脉取血，检测血常规；取胸腺和脾脏并称重，计算脏器指数，HE染色观察胸腺和脾脏形态；取肝脏、肌肉，测定肝糖原、肌糖原含量；取股骨，流式细胞术检测骨髓有核细胞凋亡。结果 与空白对照组相比，模型对照组大鼠出现活动减少，皮毛无光泽，呼吸短促等症状；且力竭游泳时间缩短，肝糖原、肌糖原含量降低，血中红细胞、白细胞、血红蛋白、血小板及红细胞压积均下降，骨髓有核细胞减少，骨髓有核细胞凋亡率上升，脾指数和胸腺指数降低（P＜0.01）；同时，脾脏结节和淋巴细胞数减少，红、白髓线不明，大量红细胞渗出，胸腺萎缩，分叶不清晰，皮质变薄，皮质细胞排列不紧密。与模型对照组相比，归芪益元膏高、中、低剂量组大鼠力竭游泳时间增加，肝糖原、肌糖原含量增加，血中红细胞、白细胞、血红蛋白、血小板及红细胞压积增加，骨髓有核细胞增加，骨髓有核细胞凋亡率下降，脾指数和胸腺指数增加（P＜0.01）；同时，脾脏结节和淋巴细胞数明显增多，红、白髓较清晰，胸腺分叶较清晰，可见皮质及髓质，皮质内细胞较模型组明显增多。结论 归芪益元膏能有效改善气血两虚证候，这可能与其提高机体能量供应，改善骨髓造血功能和增强免疫功能等有关。
Abstract:
Objective To explore the pharmacodynamics effect of Guiqi Yiyuan ointment on model rats with deficiency of both qi and blood.Methods The rat model with deficiency of both qi and blood was established by "dietary restriction + loaded swimming" and hydracetion injection, modeling and administration were carried out simultaneously, and Guiqi Yiyuan ointment was given for gastric intervention. Syndrome indexes such as ear margin, nose and lip color and mental state of rats were observed, and exhaustive swimming time was measured; blood was taken from abdominal aorta and blood routine was detected. Thymus and spleen were taken and weighed, organ index was calculated, and the morphology of thymus and spleen was observed by HE staining; liver and muscle were taken to measure the content of liver glycogen and muscle glycogen. The femur was taken, the apoptosis of bone marrow nucleated cells was detected by flow cytometry.Results Compared with the blank control group, the rats in the model control group had symptoms such as decreased activity, dull fur, shortness of breath, shortened exhaustive swimming time, as well as decreased liver glycogen and muscle glycogen content, decreased erythrocytes, leukocytes, hemoglobin, platelets and hematocrit in blood, decreased bone marrow nucleated cells, increased apoptosis in bone marrow nucleated cells, decreased spleen index and thymus index (P<0.01); meanwhile, with decreased spleen nodules and lymphocytes, unknown red and white pulp lines, a large number of red blood cell exudation, thymus atrophy, unclear lobulation, thinner cortex and arranged loosely cortical cells. Compared with the model control group, the rats in the Guiqi Yiyuan ointment with high, middle and low dose groups had symptoms such as increased exhaustive swimming time, liver glycogen and muscle glycogen contents, increased red blood cells, white blood cells, hemoglobin, platelets and hematocrit, increased bone marrow nucleated cells, decreased apoptosis in bone marrow nucleated cells, increased spleen index and thymus index (P<0.01); manwhile, with increased spleen nodules and lymphocytes, clear red and white pulp, clear thymus lobulation, visible cortex and medulla, and significantly increased cells in cortex.Conclusion Guiqi Yiyuan ointment can effectively improve the deficiency of both qi and blood syndrome, which may be related to its energy supply, bone marrow hematopoietic function and enhance immune function.

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[1]苏 苗,马若飞,李金田,等.归芪益元膏对力竭游泳法所致气虚模型小鼠的影响[J].医学信息,2023,36(12):80.[doi:10.3969/j.issn.1006-1959.2023.12.015]
　SU Miao,MA Ruo-fei,LI Jin-tian,et al.Effect of Guiqi Yiyuan Ointment on Qi Deficiency Model Mice Induced by Exhaustive Swimming Method[J].Journal of Medical Information,2023,36(16):80.[doi:10.3969/j.issn.1006-1959.2023.12.015]

更新日期/Last Update: 1900-01-01