[1]翟 堃,刘絮影,李锦存,等.转录组测序筛选口腔鳞状细胞癌中差异表达环状RNA[J].医学信息,2024,37(12):1-6.[doi:10.3969/j.issn.1006-1959.2024.12.001]
 ZHAI Kun,LIU Xu-ying,LI Jin-cun,et al.Transcriptome Sequencing in Screening Differentially Expressed Circular RNAs in Oral Squamous Cell Carcinoma[J].Journal of Medical Information,2024,37(12):1-6.[doi:10.3969/j.issn.1006-1959.2024.12.001]
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转录组测序筛选口腔鳞状细胞癌中差异表达环状RNA()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年12期
页码:
1-6
栏目:
生物信息学
出版日期:
2024-06-15

文章信息/Info

Title:
Transcriptome Sequencing in Screening Differentially Expressed Circular RNAs in Oral Squamous Cell Carcinoma
文章编号:
1006-1959(2024)11-0001-06
作者:
翟 堃刘絮影李锦存
(1.宁夏医科大学总医院口腔颌面外科,宁夏 银川 750003;2.宁夏医科大学口腔医学院,宁夏 银川 750004;3.深圳南油麦芽口腔门诊部,广东 深圳 518054)
Author(s):
ZHAI KunLIU Xu-yingLI Jin-cunet al.
(1.Department of Oral and Maxillofacial Surgery,General Hospital of Ningxia Medical University,Yinchuan 750003,Ningxia,China;2.School of Stomatology,Ningxia Medical University,Yinchuan 750004,Ningxia,China;3.Maiya Dental Clinic of Nanyou,Shenzhen 518054,Guangdong,China)
关键词:
口腔鳞状细胞癌环状RNA转录组测序
Keywords:
Oral squamous cell carcinomaCircular RNATranscriptome sequencing
分类号:
R739.8
DOI:
10.3969/j.issn.1006-1959.2024.12.001
文献标志码:
A
摘要:
目的 利用转录组测序技术对口腔鳞状细胞癌(OSCC)组织进行测序,筛选OSCC中差异表达的环状RNA(circRNA),并探讨可能与OSCC相关的信号通路。方法 收集3对OSCC患者的癌及癌旁正常组织,提取总RNA,构建circRNA文库,对其进行转录组测序,获得circRNA表达谱,筛选差异表达circRNA并进行GO和KEGG分析;构建ceRNA网络,分析预测circRNA在OSCC中的作用,并利用cytoscape软件对网络进行可视化。结果 本研究鉴定出了差异表达的circRNA281个,上调的有33个,下调的有248个。GO富集分析中主要参与细胞器组织的调节、GTP酶活性的调节;细胞组分主要在锚定连接、P小体;分子功能在核苷三磷酸酶调节活性、GTPase激活剂活性等生物学过程;KEGG主要富集在肌动蛋白细胞骨架的调节、调节干细胞多能性的信号通路、ErbB信号通路、细胞粘附连接等通路。最终筛选出7个差异表达的circRNA、2个差异表达的miRNA和3个差异表达的mRNA。结论 通过转录组测序鉴定出口腔鳞状细胞癌circRNA几乎全部为的外显子circRNA,主要富集在肌动蛋白细胞骨架的调节、GTP酶活性的调节等通路。
Abstract:
Objective To screen the differentially expressed circular RNA (circRNA) in oral squamous cell carcinoma (OSCC) by transcriptome sequencing, and to explore the possible signaling pathways related to OSCC.Methods Three pairs of cancer and paracancerous normal tissues of OSCC patients were collected. CircRNA library was constructed after extracting total RNA, then transcriptomic sequencing was carried out. The profile of circRNA was identified and annotated to obtain differentially expressed circRNAs, which were analyzed by GO and KEGG. Meanwhile, the ceRNA network was constructed to analyze and predict the role of circRNA in OSCC, and the network was visualized by cytoscape software.Results In this study, 281 differentially expressed circRNAs were identified, of which 33 were up-regulated and 248 were down-regulated. GO enrichment analysis was mainly involved in the regulation of organelle tissue and GTPase activity. The cell components were mainly anchored junctions and P bodies. Molecular function in nucleoside triphosphatase regulatory activity, GTPase activator activity and other biological processes; KEGG was mainly enriched in the regulation of actin cytoskeleton, signaling pathways regulating stem cell pluripotency, ErbB signaling pathway, cell adhesion and connection. Finally, 7 differentially expressed circRNAs, 2 differentially expressed miRNAs and 3 differentially expressed mRNAs were screened.Conclusion Transcriptome sequencing identified that almost all of the oral squamous cell carcinoma circRNA are exon circRNA, which are mainly enriched in the regulation of actin cytoskeleton and the regulation of GTPase activity.

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更新日期/Last Update: 1900-01-01