[1]孙 欣,贾英田,李时超,等.基于网络药理学及实验验证探讨益气健脾抗癌方治疗大肠癌的作用机制[J].医学信息,2025,38(10):21-30.[doi:10.3969/j.issn.1006-1959.2025.10.004]
　SUN Xin,JIA Yingtian,LI Shichao,et al.Mechanism of Yiqi Jianpi Anticancer Prescription in the Treatment of Colorectal Cancer Based on Network Pharmacology and Experimental Verification[J].Journal of Medical Information,2025,38(10):21-30.[doi:10.3969/j.issn.1006-1959.2025.10.004]
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基于网络药理学及实验验证探讨益气健脾抗癌方治疗大肠癌的作用机制()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
38卷
期数:
2025年10期
页码:
21-30
栏目:
中医药信息学
出版日期:
2025-05-15

文章信息/Info

Title:
Mechanism of Yiqi Jianpi Anticancer Prescription in the Treatment of Colorectal Cancer Based on Network Pharmacology and Experimental Verification
文章编号:
1006-1959（2025）10-0021-10
作者:
孙 欣1; 贾英田1; 李时超1; 王勤莎2; 谢 飞1; 吕其骏1; 何 涛1; 李五生1
1.西南医科大学附属中医医院肛肠科，四川 泸州 646000；2.贵州中医药大学第一临床医学院，贵州 贵阳 550000
Author(s):
SUN Xin1;  JIA Yingtian1;  LI Shichao1;  WANG Qinsha2;  XIE Fei1;  LYU Qijun1;  HE Tao1;  LI Wusheng1
1.Anorectal Department of the Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University, Luzhou 646000, Sichuan, China;2.The First Clinical Medical College, Guizhou University of Traditional Chinese Medicine, Guiyang 550000, Guizhou, China
关键词:
益气健脾抗癌方; 大肠癌; 网络药理学; 分子对接; 细胞凋亡
Keywords:
Yiqi Jianpi anticancer prescription;  Colorectal cancer;  Network pharmacology;  Molecular docking;  Apoptosis
分类号:
R735.3
DOI:
10.3969/j.issn.1006-1959.2025.10.004
文献标志码:
A
摘要:
目的 基于网络药理学及实验验证探讨益气健脾抗癌方治疗大肠癌的物质基础及作用机制。方法 利用网络药理学工具筛选出益气健脾抗癌方治疗大肠癌的主要成分、潜在靶点及核心通路；建立肠癌小鼠荷瘤模型，通过益气健脾抗癌方和化疗药物的干预，采用免疫组化、Tunel染色验证网络药理学预测的主要靶点，并观察小鼠生存期。结果 益气健脾抗癌方中共有124种化学成分，药物与疾病共同靶点52个；在PPI网络图中，CASP3、AKT1、MYC、IL6、CCND1、HIF1A、JUN、STAT3、EGFR、ERBB2是度值最靠前的节点；治疗大肠癌的主要成分包括槲皮素、木犀草素、山奈酚、川陈皮素、黄芩素等；KEGG通路富集分析表明铂类耐药是靶点富集最多的核心通路，富集的靶点为P21、CASP3、CASP8、CASP9、BAX、EPK、BCL2等，主要与细胞凋亡相关；分子对接显示关键成分与靶点间均具有结合力；动物实验证明，与对照组、中药组及化疗组相比，联合用药组的瘤体体积明显缩小（P＜0.05）、瘤体质量减轻（P＜0.05），且瘤体中Tunel染色的凋亡阳性细胞比例明显升高（P＜0.05），Bcl-2蛋白水平明显下调（P＜0.05），Bax、Caspase-3、Caspase-8、Caspase-9的表达显著上升（P＜0.05）。结论 益气健脾抗癌方通过多靶点、多成分、多途径发挥抗大肠癌的作用，主要机制可能与联合化疗促进肿瘤细胞凋亡增强化疗疗效有关。
Abstract:
Objective To explore the material basis and mechanism of Yiqi Jianpi anticancer prescription in the treatment of colorectal cancer based on network pharmacology and experimental verification. Methods Using network pharmacology tools to screen out the main components, potential targets and core pathways of Yiqi Jianpi anticancer prescription in the treatment of colorectal cancer; the tumor-bearing model of colorectal cancer mice was established. The main targets predicted by network pharmacology were verified by immunohistochemistry and Tunel staining through the intervention of Yiqi Jianpi anticancer prescription and chemotherapeutic drugs, and the survival time of mice was observed. Results Network pharmacology showed that Yiqi Jianpi anticancer prescription contained 124 chemical components and 52 drug-disease co-targets; CASP3, AKT1, MYC, IL6, CCND1, HIF1A, JUN, STAT3, EGFR, ERBB2 were the most advanced nodes in the PPI network diagram; the main components for the treatment of colorectal cancer included quercetin, luteolin, kaempferol, nobiletin, baicalein, etc. KEGG pathway enrichment analysis showed that Platinum drug resistance was the core pathway with the most enriched targets, and the enriched targets were P21, CASP3, CASP8, CASP9, BAX, EPK, BCL2, etc., they were mainly associated with apoptosis. Molecular docking showed that there was binding force between key components and targets. Animal experiments showed that compared with the control group, the Chinese medicine group and the chemotherapy group, the tumor volume of the combined treatment group was significantly reduced (P<0.05), the tumor weight was reduced (P<0.05), and the proportion of Tunel-stained apoptotic positive cells in the tumor was significantly increased (P<0.05), the Bcl-2 protein level was significantly down-regulated (P<0.05), and the expression of Bax, Caspase-3, Caspase-8 and Caspase-9 was significantly increased (P<0.05). Conclusion Yiqi Jianpi anticancer prescription plays an anti-colorectal cancer role through multiple targets, multiple components and multiple pathways. The main mechanism may be related to the combination of chemotherapy to promote tumor cell apoptosis and enhance the efficacy of chemotherapy.

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更新日期/Last Update: 1900-01-01