[1]郝 静,宋华春.PGM5-AS1表达和乳腺癌转移等恶性特征相关性的研究[J].医学信息,2026,39(10):21-26.[doi:10.3969/j.issn.1006-1959.2026.10.003]
　HAO Jing,SONG Huachun.Study on the Correlation Between PGM5-AS1 Expression and Malignant Characteristicsof Breast Cancer Metastasis[J].Journal of Medical Information,2026,39(10):21-26.[doi:10.3969/j.issn.1006-1959.2026.10.003]
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PGM5-AS1表达和乳腺癌转移等恶性特征相关性的研究()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
39卷
期数:
2026年10期
页码:
21-26
栏目:
生物信息学
出版日期:
2026-05-15

文章信息/Info

Title:
Study on the Correlation Between PGM5-AS1 Expression and Malignant Characteristicsof Breast Cancer Metastasis
文章编号:
1006-1959（2026）10-0021-06
作者:
郝 静; 宋华春
义乌市中心医院甲状腺乳腺外科，浙江 义乌 322000
Author(s):
HAO Jing;  SONG Huachun
Department of Thyroid and Breast Surgery, Yiwu Central Hospital, Yiwu 322000, Zhejiang, China
关键词:
伴有腋窝淋巴结转移乳腺癌; PGM5-AS1; 增殖; 迁移; 侵袭
Keywords:
Breast cancer with axillary lymph node metastasis;  PGM5-AS1;  Proliferation;  Migration;  Invasion
分类号:
R730.5
DOI:
10.3969/j.issn.1006-1959.2026.10.003
文献标志码:
A
摘要:
目的 探讨PGM5-AS1在伴有腋窝淋巴结转移乳腺癌中的表达情况及其对转移性乳腺癌细胞增殖、迁移、侵袭的影响。方法 qRT-PCR检测50例伴有腋窝淋巴结转移乳腺癌组织及对应癌旁组织、转移性乳腺癌细胞系（MDA-MB-231细胞）、正常的乳腺细胞系（MCF-10A细胞）中PGM5-AS1 mRNA的表达；通过重组质粒过表达MDA-MB-231细胞PGM5-AS1，采用CCK8法、细胞划痕实验、Transwell侵袭实验分别检测过表达PGM5-AS1后MDA-MB-231细胞增殖、迁移、侵袭能力的变化。结果 在伴有腋窝淋巴结转移乳腺癌及转移性MDA-MB-231细胞中，PGM5-AS1的mRNA表达水平低于对应的癌旁正常组织及正常乳腺的MCF-10A细胞（P＜0.05）；PGM5-AS1过表达后，与PGM5-AS1阴性对照组相比，PGM5-AS1 OE组MDA-MB-231细胞增殖能力降低，过表达组转染24、48、72、96 h 后的细胞增殖活力低于PGM5-AS1阴性对照组（P＜0.01）；PGM5-AS1 OE组的划痕愈合率低于PGM5-AS1阴性对照组（P＜0.05）；PGM5-AS1过表达后细胞侵袭降低，Transwell侵袭实验检测结果显示，未被处理过的MDA-MB-231细胞穿膜细胞数为（598.00±5.28）个，高于PGM5-AS1 OE组的（142.00±3.21）个，统计学意义显著（P＜0.01）。结论 在伴有腋窝淋巴结转移乳腺癌中，PGM5-AS1的表达水平上调并发挥抑癌基因的作用，通过上调PGM5-AS1水平，可以有效抑制乳腺癌细胞的增殖、迁移和侵袭能力，其有望成为乳腺癌治疗的潜在靶点。
Abstract:
Objective To investigate the expression of PGM5-AS1 in breast cancer with axillary lymph node metastasis and its effects on the proliferation, migration, and invasion of metastatic breast cancer cells. Methods qRT-PCR was used to detect the expression of PGM5-AS1 mRNA in 50 breast cancer tissues with axillary lymph node metastasis and corresponding adjacent tissues, metastatic breast cancer cell lines (MDA-MB-231 cells) and normal breast cell lines (MCF-10A cells). PGM5-AS1 was overexpressed in MDA-MB-231 cells using a recombinant plasmid. The changes in proliferation, migration, and invasion abilities of MDA-MB-231 cells after PGM5-AS1 overexpression were detected using the CCK8 assay, scratch wound assay, and Transwell invasion assay, respectively. Results In breast cancer with axillary lymph node metastasis and in metastatic MDA-MB-231 cells, the mRNA expression level of PGM5-AS1 was lower than that in the corresponding adjacent normal tissues and the normal breast MCF-10A cells (P<0.05). after overexpression of PGM5-AS1, compared with PGM5-AS1 negative control group, the proliferation ability of MDA-MB-231 cells in PGM5-AS1 OE group was decreased, and the proliferation activity of MDA-MB-231 cells in overexpression group at 24, 48, 72 and 96 h after transfection was lower than that in PGM5-AS1 negative control group (P<0.01). The scratch healing rate of PGM5-AS1 OE group was lower than that of PGM5-AS1 negative control group (P<0.05). After overexpression of PGM5-AS1, cell invasion was reduced. Transwell invasion assay showed that the number of transmembrane cells in untreated MDA-MB-231 cells was (598.00±5.28), which was higher than (142.00±3.21) in PGM5-AS1 OE group, with statistical significance (P<0.01). Conclusion In breast cancer with axillary lymph node metastasis, the expression level of PGM5-AS1 is upregulated and acts as a tumor suppressor gene. By upregulating the level of PGM5-AS1, the proliferation, migration, and invasion abilities of breast cancer cells can be effectively inhibited, making it a potential therapeutic target for breast cancer.

参考文献/References:

[1]Britt KL,Cuzick J,Phillips KA.Key steps for effective breast cancer prevention[J].Nature Reviews Cancer,2020,20(8):417-436.[2]Huang Y,Tong Z,Chen K,et al.Interpretation of Breast Cancer Screening Guideline for Chinese Women[J].Cancer Biol Med,2019 16(4):825-835.[3]Siegel RL,Miller KD,Jemal A.Cancer statistics[J].CA Cancer J Clin,2019,69(1):7-34. [4]Pastushenko I,Blanpain C.EMT transition states during tumor progression and metastasis[J].Trends in Cell Biology,2019,29(3):212-226.[5]Zhang T,Hu H,Yan G,et al.Long non-coding RNA and breast cancer[J].Technology in Cancer Research & Treatment,2019,18:1533033819843889.[6]Padroni L,De Marco L,Fiano V,et al.Identifying MicroRNAs Suitable for Detection of Breast Cancer:A Systematic Review of Discovery Phases Studies on MicroRNA Expression Profiles[J].International Journal of Molecular Sciences,2023,24(20):15114.[7]Sareyeldin RM,Gupta I,Al-Hashimi I,et al.Gene Expression and miRNAs Profiling:Function and Regulation in Human Epidermal Growth Factor Receptor 2(HER2)-Positive Breast Cancer[J].Cancers (Basel),2019,11(5):646.[8]Ding J,Wu W,Yang J,et al.Long non-coding RNA MIF-AS1 promotes breast cancer cell proliferation,migration and EMT process through regulating miR-1249-3p/HOXB8 axis[J].Pathol Res Pract,2019,215(7):152376. [9]万金华,陈会平.长链非编码RNA GAS5在卵巢癌中的表达及对肿瘤侵袭的影响[J].实用医学杂志,2016,32(24):4062-4065.[10]Keshavarz M,Asadi MH.Long non-coding RNA ES1 controls the proliferation of breast cancer cells by regulating the Oct4/Sox2/miR-302 axis[J].FEBS J,2019,286(13):2611-2623.[11]Safari MR,Mohammad RF,Dehghan A,et al.Genomic variants within the long non-coding RNA H19 confer risk of breast cancer in Iranian population[J].Gene,2019,701:121-124. [12]Shin VY,Chen J,Cheuk IW,et al.Long non-coding RNA NEAT1 confers oncogenic role in triple-negative breast cancer through modulating chemoresistance and cancer stemness[J].Cell Death Dis,2019,10(4):270. [13]Mourtada-Maarabouni M,Pickard MR,Hedge VL,et al.GAS5,a non-protein-coding RNA,controls apoptosis and is downregulated in breast cancer[J].Oncogene,2009,28(2):195-208. [14]Zhou Y,Wang C,Liu X,et al.Long non-coding RNA HOTAIR enhances radioresistance in MDA-MB231 breast cancer cells[J].Oncol Lett,2017,13(3):1143-1148.[15]Deng J,Yang M,Jiang R,et al.Long Non-Coding RNA HOTAIR Regulates the Proliferation,Self-Renewal Capacity,Tumor Formation and Migration of the Cancer Stem-Like Cell (CSC) Subpopulation Enriched from Breast Cancer Cells[J].PLoS One,2017,12(1):e0170860. [16]Eiro N,Gonzalez LO,Fraile M,et al.Breast cancer tumor stroma:cellular components, phenotypic heterogeneity, intercellular communication, prognostic implications and therapeutic opportunities[J].Cancers,2019,11(5):664.[17]Duijf PHG,Nanayakkara D,Nones K,et al.Mechanisms of Genomic Instability in Breast Cancer[J].Trends Mol Med,2019,25(7):595-611.[18]Venema CM,Bense RD,Steenbruggen TG,et al.Consideration of breast cancer subtype in targeting the androgen receptor[J].Pharmacol Ther,2019,200:135-147.[19]de Ligt KM,van Egdom LSE,Koppert LB,et al.Opportunities for personalised follow-up care among patients with breast cancer:A scoping review to identify preference-sensitive decisions[J].Eur J Cancer Care (Engl),2019,28(3):e13092.[20]杨天敬,唐瑞骏,刘海洋,等.长链非编码RNA葡萄糖磷酸变位酶样蛋白5反义1通过靶向调控微小RNA-484对乳腺癌细胞的影响[J].中华实验外科杂志,2020,37(9):1708-1711.[21]Ahmad M,Weiswald LB,Poulain L,et al.Involvement of lncRNAs in cancer cells migration,invasion and metastasis:cytoskeleton and ECM crosstalk[J].Journal of Experimental & Clinical Cancer Research,2023,42(1):173.

更新日期/Last Update: 1900-01-01