[1]李 璟,蒋 娜,王晶晶,等.右美托咪定对H9C2心肌细胞缺氧/复氧损伤的保护作用[J].医学信息,2018,31(09):83-86.[doi:10.3969/j.issn.1006-1959.2018.09.025]
　LI Jing,JIANG Na,WANG Jing-jing,et al.Protective Effects of Dexmedetomidine on Hypoxia/Reoxygenation Injury in H9C2 Cardiomyocytes[J].Journal of Medical Information,2018,31(09):83-86.[doi:10.3969/j.issn.1006-1959.2018.09.025]
点击复制

右美托咪定对H9C2心肌细胞缺氧/复氧损伤的保护作用()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年09期
页码:
83-86
栏目:
论著
出版日期:
2018-05-01

文章信息/Info

Title:
Protective Effects of Dexmedetomidine on Hypoxia/Reoxygenation Injury in H9C2 Cardiomyocytes
文章编号:
1006-1959（2018）09-0083-04
作者:
李 璟; 蒋 娜; 王晶晶; 马兴建; 王家宾
解放军第五一三医院麻醉科，甘肃 兰州 732750
Author(s):
LI Jing; JIANG Na; WANG Jing-jing; MA Xing-jian; WANG Jia-bin
Department of Anesthesiology,513 PLA Hospital,Lanzhou 732750,Gansu,China
关键词:
右美托咪定; H9C2; 缺氧/复氧; 细胞凋亡
Keywords:
Key words：Dexmedetomidine; H9C2; Hypoxia/Reoxygenation; Apoptosis
分类号:
R917
DOI:
10.3969/j.issn.1006-1959.2018.09.025
文献标志码:
A
摘要:
目的 观察右美托咪定对H9C2心肌细胞缺氧/复氧损伤的保护作用。方法 H9C2心肌细胞随机分为三组：正常对照组（Control）、缺氧/复氧组（H/R）、Dex（5 μmol/L）干预H/R组。Dex干预H/R组先用Dex预处理6 h后，再经缺氧/复氧处理。采用倒置显微镜观察各组H9C2心肌细胞形态的变化，检测各组细胞培养基中LDH的含量，CCK-8法检测各组H9C2心肌细胞的存活率，试剂盒检测caspase-3活性，TUNEL法检测细胞凋亡并计算凋亡指数。结果 与Control组相比，H/R组细胞皱缩，大量死亡，细胞活力降低，培养基中LDH含量增加，caspase-3活性增加，细胞凋亡指数增加，统计学意义显著（P＜0.01）；Dex预处理可明显改善H/R处理后细胞形态，提高细胞活力，减少LDH含量和caspase-3活性，降低细胞凋亡指数，统计学意义显著（P＜0.01）。结论 Dex可通过减少H9C2心肌细胞缺氧/复氧引起的细胞凋亡减轻损伤。
Abstract:
Abstract：Objective To observe the protective effects of dexmedetomidine on hypoxia/reoxygenation injury of H9C2 cardiomyocytes. Methods H9C2 cardiomyocytes were randomly divided into three groups: normal control group(Control),hypoxia/reoxygenation group (H/R)and Dex(5μmol/L)intervention group.Dex intervention in group H/R was treated with Dex pretreatment for 6 h before anoxia/ reoxygenation.The morphologic changes of H9C2 cardiomyocytes in each group were observed by inverted microscope.The content of LDH in the cell culture medium of each group was detected.The survival rate of H9C2 cardiomyocytes in each group was detected by CCK-8 method.The activity of caspase-3 was detected by the kit.The apoptosis was detected by TUNEL method and the apoptosis index was calculated.Results Compared with the Control group,the cells in the H/R group were shrinking,a large number of deaths, the decrease of cell vitality,the increase of LDH content in the medium,the increase of the activity of caspase-3,the increase of the apoptosis index,and the significant statistical significance(P<0.01).Dex pretreatment could obviously improve the morphology of the cells after H/R treatment,raise the vitality of the cells,reduce the content of LDH and caspase-3 activity decreased the apoptotic index,and the difference was statistically significant(P<0.01).Conclusion Dex can reduce the injury of H9C2 cardiomyocytes induced by hypoxia/reoxygenation.

参考文献/References:

[1]Hausenloy DJ,Yellon DM.Myocardial ischemia-reperfusion injury:a neglected therapeutic target[J].J Clin Invest,2013,123(1): 92-100.
[2]Hausenloy DJ,Yellon DM.Ischaemic conditioning and reperfusion injury[J].Nat Rev Cardiol,2016,13(4):193-209.
[3]Morciano G,Giorgi C,Bonora M,et al.Molecular identity of the mitochondrial permeability transition pore and its role in ischemia-reperfusion injury[J].J Mol Cell Cardiol,2015(78):142-153.
[4]姜翠翠,夏满莉,王敏,等.右美托咪定预处理减轻离体大鼠心脏缺血/再灌注损伤的线粒体相关机制[J]. 浙江大学学报（医学版）,2013,42(3):326-330.
[5]Guler L,Bozkirli F,Bedirli N,et al.Comparison of the effects of dexmedetomidine vs.Ketamin in cardiac ischemial reperfusion injury in rat-preliminary study[J].Adv Clin Exp Med,2014,23(5):683-689.
[6]Devasya A,Sarpangala M.Dexmedetomidine:a review of a newer sedative in dentistry[J].J Clin Pediatr Dent,2015,39(5):401-409.
[7]Terashima Y,Sato T,Yano T,et al.Roles of phospho-GSK-3β in myocardial protection afforded by activation of the mitochondrial K ATP channel[J].J Mol Cell Cardiol,2010,49(5):762-770.
[8]Takemura G,Kanoh M,Minatoquchi S,et al.Cardiomyocyte apoptosis in the failing heart-a critical review from definition and classification of cell death[J].Int J Cardiol,2013,167(6): 2373-2386.
[9]Zhang C,Huang Z,Gu J,et al.Fibroblast growth factor 21 protects the heart from apoptosis in a diabetic mouse model via extracellular signal-regulated kinase 1/2-dependent signalling pathway[J].Diabetologia,2015,58(8):1937-1948.
[10]Wang X,Zhang Y,Wang H,et al.MicroRNA-145 aggravates hypoxia-induced injury by targeting Rac1 in H9c2 cells[J].Cell Physiol Biochem,2017,43(5):1974-1986.
[11]Salani B,Ravera S,Fabbi P,et al.Glibenclamide mimics metabolic effects of metformin in H9c2 cells[J].Cell Physiol Biochem,2017,43(3):879-890.
[12]Major JL,Salih M,Tuana BS.E2F6 protein levels modulate drug induced apoptosis in cardiomyocytes[J].Cell Signal,2017(40):230-238.
[13]Qian ZQ,Wang YW,Li YL,et al.Icariin prevents hypertension-induced cardiomyocyte apoptosis through the mitochondrial apoptotic pathway[J].Biomed Pharmacother,2017(88):823-831.

相似文献/References:

[1]杨进斌,韩建阁.右美托咪定复合丙泊酚和芬太尼在无痛人流术中的应用观察[J].医学信息,2018,31(03):134.[doi:10.3969/j.issn.1006-1959.2018.03.047]
　YANG Jin-bin,HAN Jian-ge.Application of Dexmetomidine Combined with Propofol and Fentanyl in Painless Induced Abortion[J].Journal of Medical Information,2018,31(09):134.[doi:10.3969/j.issn.1006-1959.2018.03.047]
[2]侯 江.舒芬太尼联合右美托咪定用于临床麻醉的效果观察[J].医学信息,2018,31(03):144.[doi:10.3969/j.issn.1006-1959.2018.03.051]
　HOU Jiang.Clinical Observation on the Effect of Sufendate Combined with Dexlansoprazole in Clinical Anesthesia[J].Journal of Medical Information,2018,31(09):144.[doi:10.3969/j.issn.1006-1959.2018.03.051]
[3]陶 晶,王 鑫,黄 擘,等.右美托咪定对截肢术后发生谵妄的疗效观察[J].医学信息,2018,31(04):123.[doi:10.3969/j.issn.1006-1959.2018.04.043]
　TAO Jing,WANG Xin,HUANG Bo,et al.Therapeutic Effect of Dexmedetomidine on Delirium after Amputation[J].Journal of Medical Information,2018,31(09):123.[doi:10.3969/j.issn.1006-1959.2018.04.043]
[4]江亚楠,梁永新,施彩凤,等.右美托咪定在大鼠脊髓缺血再灌注损伤中的 保护作用及机制研究[J].医学信息,2018,31(07):69.[doi:10.3969/j.issn.1006-1959.2018.07.022]
　JIANG Ya-nan,LIANG Yong-xin,SHI Cai-feng,et al.Protective Effects and Mechanism of Dexmedetomidine on Spinal Cord Ischemia-Reperfusion Injury in Rats[J].Journal of Medical Information,2018,31(09):69.[doi:10.3969/j.issn.1006-1959.2018.07.022]
[5]孙素花,宋建防,褚海辰,等.右美托咪定经鼻给药在胸腔镜肺叶切除术中的疗效观察[J].医学信息,2018,31(07):78.[doi:10.3969/j.issn.1006-1959.2018.07.025]
　SUN Su-hua,SONG Jian-fang,CHU Hai-chen,et al.Efficacy of Dexmetomidine in Thoracoscopic Lobectomy by Nasal Administration[J].Journal of Medical Information,2018,31(09):78.[doi:10.3969/j.issn.1006-1959.2018.07.025]
[6]王绪飞,梁永新.右美托咪定复合布比卡因骶管阻滞对预防七氟醚 小儿麻醉苏醒期躁动的观察分析[J].医学信息,2018,31(08):78.[doi:10.3969/j.issn.1006-1959.2018.08.024]
　WANG Xu-fei,LIANG Yong-xin.Observation of Dexmedetomidine Combined with Bupivacaine Caudal Block in Preventing Sedation in Children with Sevoflurane during Anesthesia Recovery Period[J].Journal of Medical Information,2018,31(09):78.[doi:10.3969/j.issn.1006-1959.2018.08.024]
[7]赵 亮,王绍林.右美托咪定滴鼻和七氟烷吸入对患儿麻醉前镇静和 苏醒后躁动的影响[J].医学信息,2018,31(10):124.[doi:10.3969/j.issn.1006-1959.2018.10.040]
　ZHAO Liang,WANG Shao-lin.Effects of Dexmedetomidine Dripping and Seven Halothane Inhalation on Sedation and Recovery Agitation after Anesthesia in Children[J].Journal of Medical Information,2018,31(09):124.[doi:10.3969/j.issn.1006-1959.2018.10.040]
[8]程 燕,张 宁,郭金光.右美托咪定替代瑞芬太尼对甲状腺手术 全麻患者术后恶心呕吐的影响[J].医学信息,2018,31(12):83.[doi:10.3969/j.issn.1006-1959.2018.12.025]
　CHENG Yan,ZHANGN Ning,GUO Jin-guang.Effect of Dexmedetomidine in Place of Remifentanil on Postoperative Nausea and Vomiting in General Anesthesia Patients Undergoing Thyroid Surgery[J].Journal of Medical Information,2018,31(09):83.[doi:10.3969/j.issn.1006-1959.2018.12.025]
[9]周 刚.右美托咪定改善全麻苏醒期患者血液动力学及 医院感染的疗效观察[J].医学信息,2018,31(12):128.[doi:10.3969/j.issn.1006-1959.2018.12.039]
　ZHOU Gang.Effect of Dexmedetomidine on Improving Hemodynamics and Nosocomial Infection in Patients with General Anesthesia[J].Journal of Medical Information,2018,31(09):128.[doi:10.3969/j.issn.1006-1959.2018.12.039]
[10]郑玉珍,刘海根,陈 君.右美托咪定对大鼠脑创伤后炎症反应及认知功能的影响[J].医学信息,2018,31(14):145.[doi:10.3969/j.issn.1006-1959.2018.14.044]
　ZHENG Yu-zhen,LIU Hai-gen,CHEN Jun.Effects of Dexmedetomidine on Post-traumatic Inflammatory Response and Cognitive Function in Rats[J].Journal of Medical Information,2018,31(09):145.[doi:10.3969/j.issn.1006-1959.2018.14.044]
[11]李 璟.右美托咪定减轻氧化应激改善H9C2细胞缺氧/复氧损伤[J].医学信息,2018,31(14):95.[doi:10.3969/j.issn.1006-1959.2018.14.027]
　LI Jing.Dexmedetomidine Alleviated Oxidative Stress and Improved Hypoxia/Reoxygenation Injury in H9C2 Cells[J].Journal of Medical Information,2018,31(09):95.[doi:10.3969/j.issn.1006-1959.2018.14.027]

更新日期/Last Update: 2018-05-01