[1]邬 剑,李慧玲.ELISA法和胶体金试纸条法在乙肝表面抗原 检测中的应用研究[J].医学信息,2018,31(09):155-156.[doi:10.3969/j.issn.1006-1959.2018.09.050]
　WU Jian,LI Hui-ling.Application of ELISA and Colloidal Gold Strip in the Detection of Hepatitis B Surface Antigen[J].Journal of Medical Information,2018,31(09):155-156.[doi:10.3969/j.issn.1006-1959.2018.09.050]
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ELISA法和胶体金试纸条法在乙肝表面抗原 检测中的应用研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年09期
页码:
155-156
栏目:
诊疗技术
出版日期:
2018-05-01

文章信息/Info

Title:
Application of ELISA and Colloidal Gold Strip in the Detection of Hepatitis B Surface Antigen
文章编号:
1006-1959（2018）09-0155-02
作者:
邬 剑1; 李慧玲2
1.佳木斯市妇幼保健院检验科，黑龙江 佳木斯 154002； 2.佳木斯大学附属第一医院检验科，黑龙江 佳木斯 154002
Author(s):
WU Jian1; LI Hui-ling2
1.Department of Clinical Laboratory,Jiamusi Maternal and Child Health Hospital,Jiamusi 154002,Heilongjiang,China; 2.Department of Clinical Laboratory,First Affiliated Hospital of Jiamusi University,Jiamusi 154002,Heilongjiang,China
关键词:
乙肝表面抗原; 胶体金; 乙型病毒性肝炎
Keywords:
Key words：Hepatitis B surface antigen; Colloidal gold; Viral hepatitis type B
分类号:
R446.6
DOI:
10.3969/j.issn.1006-1959.2018.09.050
文献标志码:
A
摘要:
目的 研究ELISA法和胶体金试纸条法（GICA）在乙肝表面抗原检测中的准确度和特异性，分析方法的应用价值。方法 选取本院妇幼保健院检验科在2016年5月~2017年12月收集的共计460例血样标本，分别运用ELISA法及GICA法进行检测，比较检测结果。结果 ELISA法乙肝表面抗原阳性率为47.61%，GICA法阳性率为41.96%，差异有统计学意义（P＜0.05）。ELISA法及GICA法在对乙型病毒性肝炎表面抗原检测结果上具有密切的相关性（P＜0.05）。ELISA法灵敏度和特异度均高于GICA法（P＜0.05）。结论 ELISA法在阳性率、特异度及灵敏度等指标上均较GICA法高，但由于GICA法器材更为小巧、简洁直观，且无需大型设备辅助，较为广泛地应用于普查及急诊前检查中。
Abstract:
Abstract：Objective To study the accuracy and specificity of ELISA and colloidal gold strip method in the detection of hepatitis B surface antigen.Methods A total of 460 blood samples were collected from laboratory Department of Maternal and Child Health Hospital from May 2016 to December 2017.ELISA and GICA methods were used to detect and compare the results.Results The positive rate of HBsAg in ELISA was 47.61%,and the positive rate in GICA was 41.96%,the difference was statistically significant (P＜0.05).The ELISA method and GICA method have close correlations on the results of surface antigen detection of hepatitis B virus(P＜0.05).The sensitivity and specificity of ELISA were higher than those of GICA(P＜0.05).Conclusion The ELISA method is more accurate than the GICA method in terms of positive rate,specificity,and sensitivity,but the GICA method is more compact,simple,and intuitive,and does not require large-scale equipment,and is widely used in census and pre-emergency tests.

参考文献/References:

[1]李伶俐,孙俊聪.400例乙肝表面抗原胶体金试条法与ELISA法检测结果分析[J].中国医学创新,2014,28(28):35-36.
[2]郝青华.胶体金试纸条法和ELISA法在乙肝表面抗原检测中的效果分析[J].医学信息,2014,27(34):57-57.
[3]李小云.ELISA法和胶体金免疫层析法检测乙肝表面抗原结果分析[J].医学美学美容,2015(3):225-225.
[4]曾妮,叶兴,黄河浪.乙肝免疫球蛋白联合乙肝疫苗阻断乙型肝炎母婴传播的系统评价[J].中华疾病控制杂志,2017,21(1):48-51.

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更新日期/Last Update: 2018-05-01