[1]杨 娜,周进平,林晓容,等.去睾丸老年大鼠血管钙化程度与骨代谢水平研究[J].医学信息,2019,32(04):5-7.[doi:10.3969/j.issn.1006-1959.2019.04.002]
　YANG Na,ZHOU Jin-ping,LIN Xiao-rong,et al.Study on the Degree of Vascular Calcification and Bone Metabolism in Aged Test Rats[J].Journal of Medical Information,2019,32(04):5-7.[doi:10.3969/j.issn.1006-1959.2019.04.002]
点击复制

去睾丸老年大鼠血管钙化程度与骨代谢水平研究()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年04期
页码:
5-7
栏目:
出版日期:
2019-02-15

文章信息/Info

Title:
Study on the Degree of Vascular Calcification and Bone Metabolism in Aged Test Rats
文章编号:
1006-1959（2019）04-0005-03
作者:
杨 娜1; 周进平2; 林晓容1; 徐军霞2
（1.福建中医药大学康复医学院，福建 福州 350122；2.解放军联勤保障部队第九〇〇医院，福建 福州 350025）
Author(s):
YANG Na1; ZHOU Jin-ping2; LIN Xiao-rong1; XU Jun-xia2
(1.Rehabilitation Medical College,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China;2.The 900 Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army,Fuzhou 350025,Fujian,China)
关键词:
去睾丸大鼠; 老年性骨质疏松; 血管钙化; 骨代谢
Keywords:
Testicular rats; Senile osteoporosis; Vascular calcification; Bone metabolism
分类号:
R322.7
DOI:
10.3969/j.issn.1006-1959.2019.04.002
文献标志码:
A
摘要:
目的 观察去睾丸老年大鼠血管钙化程度、骨密度（BMD）及骨代谢指标[Ⅰ型前胶原N-端前肽（PⅠNP）、骨钙素（BGP）和Ⅰ型胶原交联羧基末端肽（CTX-Ⅰ）]的变化。方法 取7月龄雄性SD大鼠16只，随机分造模组及对照组。造模组采用双侧睾丸摘除法建立大鼠骨质疏松模型，对照组进行假手术。术后6个月，处死所有大鼠，取右侧股骨以双能X线吸收仪测量BMD，并取胸主动脉用茜素红染色观察钙化情况，用酶联免疫吸附法测量血清PⅠNP、BGP和CTX-Ⅰ浓度。结果 造模组BMD低于对照组[（0.2636±0.0096）g/cm2 vs （0.3104±0.0157）g/cm2]，差异有统计学意义（P＜0.05）；造模组胸主动脉钙化明显，血清P1NP、BGP含量较对照组降低差异有统计学意义（P＜0.05），而血清CTX-Ⅰ高于对照组，差异有统计学意义（P＜0.05）。结论 通过去势法可建立老年大鼠骨质疏松模型，且骨质疏松形成过程中血管钙化程度明显增加，骨形成标志物降低，骨吸收标志物增高。
Abstract:
Objective To observevascular calcification, bone mineral density (BMD) and bone metabolism markers in testicular aged rats [type I procollagen N-terminal propeptide (PⅠNP), osteocalcin (BGP) and type I collagen cross-linked carboxy terminal peptide (CTX) -Ⅰ)] changes. Methods 16 male Sprague-Dawley rats aged 7 months were randomly divided into two groups. In the model group, the rat osteoporosis model was established by bilateral testicular extraction, and the control group was given sham operation. Six months after operation, all rats were sacrificed. BMD was measured by dual-energy X-ray absorptiometry. The thoracic aorta was stained with alizarin red to observe calcification. Serum P1NP and BGP were measured by enzyme-linked immunosorbent assay and CTX-Ⅰ concentration. Results The BMD of the model group was lower than that of the control group [(0.2636±0.0096) g/cm2 vs (0.3104±0.0157) g/cm2], the difference was statistically significant (P<0.05); The calcification of the thoracic aorta was obvious, and the serum PⅠNP and BGP levels were significantly lower than those of the control group (P<0.05), while the serum CTX-Ⅰ was higher than the control group, the difference was statistically significant (P<0.05). Conclusion Osteoporosis model can be established in the aged rats by castration method, and the degree of vascular calcification is significantly increased during the process of osteoporosis formation, bone formation markers are decreased, and bone resorption markers are increased.

参考文献/References:


[1]Szulc P.Vascular calcification and fracture risk[J].Clinical cases in mineral and bone metabolism,2015,12(2):139-141.
[2]Lampropoulos CE,Kalamara P,Konsta M,et al.Osteoporosis and vascular calcification in postmenopausal women: a cross-sectional study[J].Climacteric,2016,19(3):303-307.
[3]Svedbom A,Hernlund E,Lvergard M,et al.Osteoporosis in the European Union: a compendium of country-specific reports[J].Arch Osteoporos,2013(8):137.
[4]Kanis JA,McCloskey EV,Johansson H,et al.European guidance for the diagnosis and management of osteoporosis in postmenopausal women[J].Osteoporos Int,2013,24(1):23-57.
[5]杨永红,林明春,夏凤琼,等.骨标志物OC、CTX-1、BAP、tP1NP的检测在骨质疏松症中的临床应用[J].中外医学研究,2015,13(27):162-164.
[6]王洪复.老年性骨质疏松症病理机制与防治原则[J].中华保健医学杂志,2010,12(1):1-4.
[7]Ye C,Xu M,Wang S,et al.Decreased Bone Mineral Density Is an Independent Predictor for the Development of Atherosclerosis: A Systematic Review and Meta-Analysis[J].PLoS One,2016,11(5):e0154740.
[8]Chan JJ,Cupples LA,Kiel DP,et al.QCT Volumetric Bone Mineral Density and Vascular and Valvular Calcification: The Framingham Study[J].J Bone Miner Res,2015,30(10):1767-1774.
[9]Ozkan E,Ozkan H,Bilgic S,et al.Serum fetuin-A levels in postmenopausal women with osteoporosis[J].Turkish J Med Sci,2014,44(6):985-988.
[10]Rattazzi M,Faggin E,Buso R,et al.Atorvastatin Reduces Circulating Osteoprogenitor Cells and T-Cell RANKL Expression in Osteoporotic Women: Implications for the Bone-Vascular Axis[J].Cardiovasc Ther,2016,34(1):13-20.
[11]Shioi A.Vascular calcification-pathological mechanism and clinical application-mechanisms of vascular calcification[J].Clin Calcium,2015(25):635-643.
[12]Caffarelli C,Montagnani A,Nuti R,et al.Caffarelli Bisphosphonates, atherosclerosis and vascular calcification: update and systematic review of clinical studies[J].Clinical Interventions in Aging,2017(12):1819-1828.
[13]Rochette L,Meloux A,Rigal E,et al.The role of osteoprotegerin in the crosstalk between vessels and bone: Its potential utility as a marker of cardiometabolic diseases[J].Pharmacology & Therapeutics,2018(182):115-132.
[14]Schurgers LJ,Uitto J,Reutelingsperger CP.Vitamin K-dependent carboxylation of matrix Gla-protein: a crucial switch to control ectopic mineralization[J].Trends Mol Med,2013,19(4):217-226 .
[15]明静,胡凤琪,秦永芳,等.血液透析患者血清骨钙素水平及其与血管钙化的关系[J].医学研究杂志,2013,42(9):67-69.
[16]Ma H,Lin H,Hu Y,et al.Serum levels of osteocalcin in relation to glucose metabolism and carotid atherosclerosis in Chinese middle-aged and elderly male adults: The Shanghai Changfeng Study[J].European Journal of Internal Medicine,2014,25(3):259-264.

相似文献/References:

[1]李家德,钟永翔,陈永志,等.腰痛方颗粒联合抗骨质疏松药物治疗肝肾亏虚型老年性骨质疏松的效果[J].医学信息,2020,33(22):163.[doi:10.3969/j.issn.1006-1959.2020.22.048]
　LI Jia-de,ZHONG Yong-xiang,CHEN Yong-zhi,et al.Effect of Yaotongfang Granule Combined with Anti-osteoporosis Drugs on Senile Osteoporosis with Deficiency of Liver and Kidney[J].Journal of Medical Information,2020,33(04):163.[doi:10.3969/j.issn.1006-1959.2020.22.048]

更新日期/Last Update: 2019-03-07