[1]姚淮育,吴虹谚,何 昊,等.荟生源两种组方对小鼠经口急性毒性实验[J].医学信息,2019,32(11):72-75.[doi:10.3969/j.issn.1006-1959.2019.11.020]
 YAO Huai-yu,WU Hong-yan,HE Hao,et al.Experimental Study on Acute Toxicity of Huishengyuan to Mice through Mouth[J].Journal of Medical Information,2019,32(11):72-75.[doi:10.3969/j.issn.1006-1959.2019.11.020]
点击复制

荟生源两种组方对小鼠经口急性毒性实验()
分享到:

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年11期
页码:
72-75
栏目:
论著
出版日期:
2019-06-01

文章信息/Info

Title:
Experimental Study on Acute Toxicity of Huishengyuan to Mice through Mouth
文章编号:
1006-1959(2019)11-0072-04
作者:
姚淮育1吴虹谚2何 昊3付满玲4吴显和5
1.泸州市人民医院重症医学科,四川 泸州 646000; 2.乐山市人民医院重症医学科,四川 泸州 646000; 3.西南医科大学附属医院重症医学科,四川 泸州 646000; 4.西南医科大学护理器械研发实验室,四川 泸州 646000; 5.泸州鑫欣科技有限公司,四川 泸州 646000
Author(s):
YAO Huai-yu1WU Hong-yan2HE Hao3FU Man-ling4WU Xian-he5
1.Department of Severe Medicine,Luzhou People's Hospital, Luzhou 646000,Sichuan,China; 2.Department of Severe Medicine,Leshan People's Hospital, Luzhou 646000,Sichuan,China; 3.Department of Severe Medicine,Affiliated Hospital of Southwest Medical Universi
关键词:
荟生源经口急性毒性实验小鼠
Keywords:
Key words:HuishengyuanOral acute toxicity testMouse
分类号:
R285.5
DOI:
10.3969/j.issn.1006-1959.2019.11.020
文献标志码:
A
摘要:
目的 评价荟生源两种组方的安全性,评估其临床使用安全性,为功能性食品开发和临床安全用药提供实验依据。方法 将26只SPF级昆明小鼠采用随机数目表法随机分为3组,其中实验组1、实验组2各10只,空白组6只,雌雄各半。实验组1以荟生源0.96 g+生理盐水0.17 ml(按配方1最大溶解度进行配置,有效量接近于成人每日服用量200倍)灌胃、实验组2以荟生源0.4 g+生理盐水0.63 ml(按配方2最大溶解度进行配置,有效量接近于于成人每日标准服用量200倍),两组实际灌胃量均为0.8 ml;空白组以生理盐水0.8 ml灌胃,灌胃后连续观察7 d。比较3组一般情况、死亡情况、大体解剖及镜下特点。结果 3组小鼠体重、饮水量、活动度均正常,差异无统计学意义(P>0.05);实验过程中各组均无小鼠死亡,大体解剖肉眼观察小鼠各脏器均无变性、充血、水肿、坏死等异常现象;镜下对照组小鼠肝脏、肾脏细胞形态、结构、大小均正常,无坏死、无病理性改变。实验组1小鼠肝细胞形态、结构、大小均正常,肝小叶组织结构正常;实验组2中出现1例肝细胞胞浆疏松化。各组所有动物肾组织结构肾小管结构正常,皮质与髓质分界线清楚,无淋巴细胞浸润。结论 荟生源两种组方对小鼠无急性毒性作用,具有较好的安全性。
Abstract:
Abstract:Objective To evaluate the safety of Huishengyuan and its clinical safety, so as to provide experimental basis for the development of functional food and the safe use of drugs in clinic.Methods 26 SPF Kunming mice were randomly divided into three groups: experimental group 1 (n =10), experimental group (n=10) and blank group (n=6).Both male and female. In the experimental group 1, 0.96 g + normal saline 0.17 ml (configured according to the maximum solubility of the formula 1 and the effective amount is close to 200 times the daily dosage of the adult) was administered to the experimental group 1 and the experimental group 2 was 0.4 g+0.63 ml(of physiological saline. The maximum solubility of Formulation 2 was configured,and the effective amount was close to 200 times of the daily standard dose of adults.) The actual gastric perfusion volume was 0.8 ml; the blank group was intragastrically administered with 0.8 ml of normal saline, and was continuously observed for 7 d after gavage.Compare the general conditions, death, gross anatomy and microscopic features of the 3 groups.Results The body weight, water consumption and activity of the three groups were normal, the difference was not statistically significant (P>0.05). There was no mouse death in each group during the experiment.Gross dissection observed the mice without degeneration and congestion. Abnormal phenomena such as edema and necrosis; the morphology, structure and size of liver and kidney cells of the mice in the control group were normal, no necrosis and no pathological changes. The morphology, structure and size of hepatocytes in experimental group 1 were normal, and the structure of hepatic lobule was normal. In experimental group 2, 1 case of hepatocyte cytoplasmic loosening occurred. The renal tubular structure of all animals in each group was normal, the boundary between cortex and medulla was clear, and there was no lymphocytic infiltration.Conclusion Huishengyuan two kinds of prescription have no acute toxicity to mice, and have good safety.

参考文献/References:

[1]李光耀,刘维.化疗相关恶心呕吐的预防[J].临床药物治疗杂志,2017,15(2):90. [2]芦殿荣,芦殿香,殷玉琨,等.顺铂导致化疗相关恶心呕吐反应的中药防治[J].长春中医药大学学报,2014(4):645-647. [3]毛咏旻,王一红,谢腾,等.中西医防治顺铂引起化疗后恶心呕吐反应的研究进展[J].中华全科医学,2017,15(2):321-324. [4] [H]GPT1-1.化学药物急性毒性试验技术指导原则[S].《化学药物急性毒性试验技术指导原则》课题研究组,2005. [5]李颖,刑斌,姚秀娟,等.一种简便的小鼠活动度的检测方法[J].西北药学杂志,1995,10(1):27-29. [6]李爱国,王鑫.中西医结合防治肿瘤化疗性呕吐40例[J].中医研究,2017,30(1):34-36. [7]董晶,王玲玲,王邦才,等.养胃和络饮联合昂丹司琼治疗乳腺癌化疗后呕吐30例[J].浙江中医杂志,2019,54(1):53. [8]张雪峰,彭云云,胡燕.预防奥沙利铂化疗后恶心呕吐方案的比较[J].贵州医药,2019,43(1):67-69. [9]梁峻立,吕文艳.帕洛诺司琼与托烷司琼对预防单节段腰椎减压植骨融合内固定手术后恶心呕吐的效果比较[J].中国当代医药,2018,25(10):16-18,22. [10]丁红,施伟伟,王治宽,等.阿瑞匹坦联合托烷司琼治疗化疗所致恶心呕吐的回顾性分析[J].癌症进展,2017,15(4):408-410, 431. [11]郝建萍.六君子汤联合托烷司琼预防乳腺癌化疗呕吐不良反应的疗效观察[J].中国医药指南,2018,16(35):177. [12]崔艳东.加味小半夏汤防治肿瘤化疗后恶心、呕吐40例疗效观察[J].湖南中医杂志,2018,34(12):49-50. [13]高春明,张武,黄文彬.旋覆代赭汤加减联合盐酸格拉司琼防治化疗性恶心呕吐的效果探讨[J].数理医药学杂志,2019,32(2):237-238. [14]王艳红,王鋆泉,王芳.穴位注射氟哌利多对预防肺癌术后化疗患者恶心呕吐的影响[J].首都食品与医药,2019,26(2):43-44. [15]王晓艳,李志华.中医穴位贴敷技术对癌症患者化疗后恶心呕吐的防治效果[J].四川中医,2019,37(1):189-190. [16]周爱春,刘云霞,陈丽娟,等.穴位贴敷防治骨肉瘤患者化疗后恶心呕吐43例[J].浙江中医杂志,2018,53(12):884-885. [17]李娜,钟云萍,孙圣荣.渐进式肌肉放松训练缓解乳腺癌化疗期恶心呕吐的效果[J].上海护理,2019,19(1):10-13. [18]黄妹妹,罗瑞君.放松训练对化疗患者预期性恶心呕吐的效果观察[J].护士进修杂志,2019,34(2):171-172.

相似文献/References:

[1]付满玲,黄雨佳,刘珉甬,等.山楂代用茶对大鼠经口急性毒性实验[J].医学信息,2020,33(01):77.[doi:10.3969/j.issn.1006-1959.2020.01.024]
 FU Man-ling,HUANG Yu-jia,LIU Min-yong,et al.Experimental on Oral Acute Toxicity of Hawthorn Substitute Tea on Rats[J].Journal of Medical Information,2020,33(11):77.[doi:10.3969/j.issn.1006-1959.2020.01.024]

更新日期/Last Update: 2019-06-01