[1]张国艳,张佳田,隋洪玉.糖尿病肾病大鼠动物模型建立及INF-α干预机制研究[J].医学信息,2019,(05):101-102.[doi:10.3969/j.issn.1006-1959.2019.05.030]
 ZHANG Guo-yan,ZHANG Jia-tian,SUI Hong-yu.Establishment of Animal Model of Diabetic Nephropathy Rats and Study of INF-α Intervention Mechanism[J].Medical Information,2019,(05):101-102.[doi:10.3969/j.issn.1006-1959.2019.05.030]
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糖尿病肾病大鼠动物模型建立及INF-α干预机制研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期数:
2019年05期
页码:
101-102
栏目:
临床研究
出版日期:
2019-03-01

文章信息/Info

Title:
Establishment of Animal Model of Diabetic Nephropathy Rats and Study of INF-α Intervention Mechanism
文章编号:
1006-1959(2019)05-0101-02
作者:
张国艳1张佳田1隋洪玉2
(1.佳木斯大学附属第一医院肾内科,黑龙江 佳木斯 154003;2.佳木斯大学基础医学院,黑龙江 佳木斯 154007)
Author(s):
ZHANG Guo-yan1ZHANG Jia-tian1SUI Hong-yu2
(1.Department of Nephrology,the First Affiliated Hospital of Jiamusi University,Jiamusi 154003,Heilongjiang,China;2.School of Basic Medicine,Jiamusi University,Jiamusi 154007,Heilongjiang,China)
关键词:
高糖高脂饲料STZ糖尿病肾病大鼠动物模型TNF-α
Keywords:
High-sugar and high-fat dietSTZDiabetic nephropathyRat animal modelTNF-α
分类号:
R587.2
DOI:
10.3969/j.issn.1006-1959.2019.05.030
文献标志码:
A
摘要:
目的 探讨糖尿病肾病大鼠动物模型建立及TNF-α干预机制。方法 选择 SD大鼠60只作为对象,随机数字法分为对照组和观察组,各30只。对照组大鼠腹腔注射柠檬酸缓冲液,观察组在对照组基础上采用高糖高脂饲料联合5%葡萄糖饮水喂养,诱导胰岛素抵抗,2周后腹腔注射STZ,连续注射3 d,诱发持续高糖血症。采用酶联免疫吸附法在建模前、建模2周、建模4周检测观察组大鼠血糖、尿白蛋白、肌酐水平,同时检测两组大鼠TNF-α水平,分析糖尿病动物模型建立及TNF-α干预机制。结果 ①对照组大鼠全身状态良好,体质量增加,皮毛光滑亮泽,观察组大鼠建模成功后出现多食、多饮、多尿及生长迟缓现象,成模4周后体重低于对照组,差异有统计学意义(P<0.05);②观察组建模2周血糖水平(8.21±0.21)mmol/L,建模4周血糖水平(31.21±1.04)mmol/L,均高于对照组的(6.32±0.19)mmol/L、(6.74±0.24)mmol/L),差异有统计学意义(P<0.05);③观察组建模2周TNF-α水平为(3.03±0.23)mmol/L,建模4周TNF-α水平为(12.10±1.12)mmol/L,均高于对照组的(1.64±0.58)mmol/L、(1.69±0.62)mmol/L,差异有统计学意义(P<0.05)。结论 高糖高脂饲料联合浓度为5%葡萄糖饮水配合小剂量STZ能建立理想的糖尿病肾病大鼠动物模型,可以明确糖尿病肾病发展伴有TNF-α炎症因子水平升高,进一步为TNF-α干预机制研究奠定了基础。
Abstract:
Objective To investigate the establishment of animal models of diabetic nephropathy rats and the mechanism of TNF-a intervention. Methods 60 SD rats were selected as the subjects. The random number method was divided into control group and observation group, 30 each. Rats in the control group were intraperitoneally injected with citrate buffer. The observation group was fed with high-sugar and high-fat diet combined with 5% dextrose water to induce insulin resistance. After 2 weeks, STZ was injected intraperitoneally for 3 consecutive days,induced persistent hyperglycemia.The levels of blood glucose, urinary albumin and creatinine in the observation group were measured by enzyme-linked immunosorbent assay (ELISA) before modeling, 2 weeks of modeling, and 4 weeks of modeling. The levels of TNF-α in the two groups were also measured and TNF-a intervention mechanism. Results ①The rats in the control group had good systemic condition, the body mass increased, and the fur was smooth and shiny. The rats in the observation group showed polyphagia, polydipsia, polyuria and growth retardation after successful modeling. After 4 weeks of modeling, the body weight was lower than that of the control group,the difference was statistically significant (P<0.05); ②The observation group modeling 2 weeks blood glucose level (8.21±0.21) mmol/L, modeling 4 weeks blood glucose level (31.21±1.04) mmol/L, they were higher than the control group (6.32±0.19) mmol/L, (6.74±0.24) mmol/L, the difference was statistically significant (P<0.05); ③The level of TNF-α in the observation group was (3.03±0.23) mmol/L for 2 weeks, and the level of TNF-α was (12.10±1.12) mmol/L for 4 weeks, which was higher than that of the control group (1.64±0.58) mmol/L, (1.69±0.62) mmol/L, the difference was statistically significant (P<0.05). Conclusion The high concentration of high-fat and high-fat diet combined with 5% dextrose drinking water combined with low-dose STZ can establish an ideal animal model of diabetic nephropathy in rats. It can be confirmed that the development of diabetic nephropathy is accompanied by elevated levels of TNF-a inflammatory factors, and further intervention for TNF-α. Mechanism research laid the foundation.

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更新日期/Last Update: 2019-03-01