[1]白吉明,张 勃,李建华,等.中空纤维测定法在胃癌临床前药效研究中的应用[J].医学信息,2019,(07):93-96.[doi:10.3969/j.issn.1006-1959.2019.07.027]
 BAI Ji-ming,ZHANG Bo,LI Jian-hua,et al.Application of Hollow Fiber Assay in Preclinical Efficacy Study of Gastric Cancer[J].Medical Information,2019,(07):93-96.[doi:10.3969/j.issn.1006-1959.2019.07.027]
点击复制

中空纤维测定法在胃癌临床前药效研究中的应用()
分享到:

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期数:
2019年07期
页码:
93-96
栏目:
论著
出版日期:
2019-04-01

文章信息/Info

Title:
Application of Hollow Fiber Assay in Preclinical Efficacy Study of Gastric Cancer
文章编号:
1006-1959(2019)07-0093-04
作者:
白吉明1张 勃2李建华1王 翔1侯志伟1
(1.承德医学院第二临床医院承德市中心医院肿瘤科,河北 承德 067000;2.承德医学院附属医院呼吸科,河北 承德 067000)
Author(s):
BAI Ji-ming1ZHANG Bo2LI Jian-hua1WANG Xiang1HOU Zhi-wei1
(1.Department of Oncology,Chengde Central Hospital,the Second Clinical Hospital,Chengde Medical College,Chengde 067000,Hebei,China;2.Department of Respiratory,Affiliated Hospital of Chengde Medical College,Chengde 067000,Hebei, China)
关键词:
中空纤维测定法异种移植模型抗肿瘤试验胃癌
Keywords:
Hollow fiber assayXenograft model anti-tumor testGastric cancer
分类号:
R96
DOI:
10.3969/j.issn.1006-1959.2019.07.027
文献标志码:
A
摘要:
目的 探讨中空纤维测定法在胃癌临床前药效研究中的应用。方法 将胃癌细胞株SNU-16、 SNU-484、SNU-668分别通过中空纤维管移植于裸鼠的皮下和腹腔,随后用标准的抗癌药物紫杉醇制剂给予治疗,将每个细胞株的中空纤维的活性与异种移植物的活性进行比较。结果 使用优化的接种密度和时间表,紫杉醇治疗在胃癌细胞株SNU-16和SNU-484有效,经紫杉醇处理后的SNU-16和SNU-484胃癌细胞株减少大于50%,但在SNU-668无效。与中空纤维测定结果一致,胃癌细胞株SNU-16和SNU-484在异种移植模型中紫杉醇治疗后也表现出肿瘤消退。结论 本实验研究表明中空纤维测定法对于筛选具有抗胃癌活性的小分子物质是有效的。
Abstract:
Objective To investigate the application of hollow fiber assay in the study of preclinical efficacy of gastric cancer. Methods Gastric cancer cell lines SNU-16, SNU-484 and SNU-668 were transplanted into the subcutaneous and peritoneal cavity of nude mice through hollow fiber tubes, respectively, and then treated with the standard anticancer drug paclitaxel. The hollow fibers of each cell line were treated. The activity was compared to the activity of the xenograft. Results Using optimized seeding density and schedule, paclitaxel treatment was effective in gastric cancer cell lines SNU-16 and SNU-484, and the SNU-16 and SNU-484 gastric cancer cell lines treated with paclitaxel were reduced by more than 50%, but at SNU-668 invalid. Consistent with the results of hollow fiber assays, gastric cancer cell lines SNU-16 and SNU-484 also showed tumor regression after paclitaxel treatment in a xenograft model. Conclusion This experimental study shows that the hollow fiber assay is effective for screening small molecules with anti-gastric cancer activity.

参考文献/References:


[1]石然,徐铭恩,周青青,等.基于细胞3D打印技术的体外肿瘤模型构建研究[J].中国生物医学工程学报,2015,34(5):618-622.
[2]邓晶晶,纪圣君,陈烨.应用中空纤维测定法评价藤甲酰苷对人癌细胞生长的抑制作用[J].药学实践杂志,2014,32(4):266-269.
[3]赵敏,师霞.黄芪对急性呼吸窘迫综合征大鼠模型肺组织中SP-A的影响[J].中国临床研究,2013,5(8):1-3.
[4]张映城,颜兵,施俊,等.胃癌细胞中CD44阳性细胞具有肿瘤干细胞特征[J].中国肿瘤生物治疗杂志,2016,23(4):492-497.
[5]戚欣,张琳,李静.中空纤维法在昆明小鼠中应用——一种体内抗肿瘤药物筛选技术[J].中国海洋药物,2013,32(4):29-34.
[6]Son JY,Park SY,Kim SJ,et al.EW-7197,A Novel ALK-5 Kinase Inhibitor,Potently Inhibits Breast to Lung Metastasis.Mol Cancer Ther,2014,13(7)1:1704-1716.
[7]王丽萍,王新春.黄酮类化合物的代谢研究进展[J].中国实验方剂学杂志,2014,20(11):234-237.

更新日期/Last Update: 2019-04-01