[1]宋 静,戎建荣.碳青霉烯类耐药肠杆菌科细菌实验室检测的研究[J].医学信息,2019,(19):25-28.[doi:10.3969/j.issn.1006-1959.2019.19.009]
 SONG Jing,RONG Jian-rong.Laboratory Test of Carbapenem-resistant Enterobacteriaceae Bacteria[J].Medical Information,2019,(19):25-28.[doi:10.3969/j.issn.1006-1959.2019.19.009]
点击复制

碳青霉烯类耐药肠杆菌科细菌实验室检测的研究()
分享到:

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期数:
2019年19期
页码:
25-28
栏目:
综述
出版日期:
2019-10-01

文章信息/Info

Title:
Laboratory Test of Carbapenem-resistant Enterobacteriaceae Bacteria
文章编号:
1006-1959(2019)19-0025-04
作者:
宋 静戎建荣
(山西医科大学附属大医院检验科,山西 太原 030032)
Author(s):
SONG JingRONG Jian-rong
(Department of Clinical Laboratory,Affiliated Hospital of Shanxi Medical University,Taiyuan 030032,Shanxi,China)
关键词:
肠杆菌科细菌碳青霉烯酶表型检测碳青霉烯灭活试验
Keywords:
EnterobacteriaceaeCarbapenemasePhenotypic detectionCarbapenem inactivation test
分类号:
R33
DOI:
10.3969/j.issn.1006-1959.2019.19.009
文献标志码:
A
摘要:
碳青霉烯类耐药肠杆菌科细菌(CRE)已经成为全球性公共卫生问题,这类细菌往往伴随高致病率、高致残率、高死亡率,为临床治疗带来了极大的挑战。CRE的耐药机制主要是产生碳青霉烯酶。快速、准确地检测产碳青霉烯酶的肠杆菌科细菌,对于合理使用抗生素,预防和控制产酶菌株的传播具有重要意义。本文就实验室检测肠杆菌科细菌产碳青霉烯酶的研究方法进展作一综述。
Abstract:
Carbapenem-resistant Enterobacteriaceae (CRE) has become a global public health problem. These bacteria are often accompanied by high morbidity, high disability, and high mortality, which have brought great treatment to clinical treatment challenge. The mechanism of CRE resistance is mainly the production of carbapenemases. Rapid and accurate detection of Enterobacteriaceae bacteria producing carbapenemase is of great significance for the rational use of antibiotics to prevent and control the spread of enzyme-producing strains. This article reviews the progress of laboratory research on the detection of carbapenemase in Enterobacteriaceae.

参考文献/References:

[1]Little ML,Qin X,Zerr DM,et al.Molecular diversity in mechanisms of carbapenem resistance in paediatric Enterobacteriaceae[J].Int J Antimicrob Agents,2012,39(1):52-57. [2]Nordmann P,Poirel L.Emerging carbapenemases in Gramnegative aerobes[J].Clin Microbiol Infect,2002(8):321-331. [3]Livermore DM,Woodford N.Carbapenemases:a problem in waiting?[J].Curr Opin Microbiol,2000(3):489-495. [4]Bush K.Metallo-beta-lactamases:a class apart[J].Clin Infect Dis,1998,27(Suppl 1):S48-S53. [5]Rasmussen BA,Bush K.Carbapenem-hydrolyzing beta-lactamases[J].Antimicrob Agents Chemother,1997(41):223-232. [6]Girlich D,Poirel L,Nordmann P.Value of the modified Hodge test for detection of emergingcarbapenemases in Enterobacteriaceae[J].J Clin Microbiol,2012,50(2):477-479. [7]Pasteran F,Gonzalez LJ,Albornoz E,et al.Triton Hodge Test: Improved Protocol for Modified Hodge Test for Enhanced Detection of NDM and Other Carbapenemase Producers[J].J Clin Microbiol,2016,54(3):640-649. [8]Roth AL,Kurpiel PM,Lister PD,et al.bla(KPC) RNA expression correlates with two transcriptional start sites but not always with gene copy number in four genera of Gram-negative pathogens[J].Antimicrob Agents Chemother,2015,5(8):3936-3938. [9]Dortet L,Bréchard L,Poirel L,et al.Impact of the isolation medium for detection of carbapenemase-producing Enterobacteriaceae using an updated version of the Carba NP test[J].J Med Microbiol,2014,63(Pt 5):772-776. [10]Segawa T,Matsui M,Suzuki M,et al.Utilizing the Carba NP test as an indicator of expression level of carbapenemase genes in Enterobacteriaceae[J].J Microbiol Methods,2017(133):35-39. [11]Literacka E,Herda M,Baraniak A,et al.Evaluation of the Carba NP test for carbapenemase detection in Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp,and its practical use in the routine work of a national reference laboratory for susceptibility testing[J].Eur J Clin Microbiol Infect Dis,2017,36(11):2281-2287. [12]Akyar I,Kaya Ayas M,Karatuna O.Performance Evaluation of MALDI-TOF MS MBT STAR-BL Versus In-House Carba NP Testing for the Rapid Detection of Carbapenemase Activity in Escherichia coli and Klebsiella pneumoniae Strains[J].Microb Drug Resist,2019. [13]van der Zwaluw K,de Haan A,Pluister GN,et al.Carbapenem Inactivation Method (CIM),a simple and low-cost alternative to the Carba NP test to assess phenotypic carbapenemase activity in Gram-negativerods[J].PLoS One,2015(10):e0123690. [14]Pragasam AK,Veeraraghavan B,Bakthavatchalam YD,et al.Strengths and limitations of various screening methods for carbapenem-resistant Enterobacteriaceae including new method recommended by clinical and laboratory standards institute,2017:A tertiary care experience[J].Indian J Med Microbiol,2017,35(1):116-119. [15]马玉兰,宋文杰,梁屹,等.CIM与mCIM筛选肠杆菌科细菌产碳青霉烯酶能力比较[J].河北医科大学学报,2018,39(8):943-948. [16]Aktas E,Malkosoglu G,Otlu B,et al.Evaluation of the Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Gram-Negative Bacteria in Comparison with the RAPIDEC CARBA NP[J].Microb Drug Resist,2017,23(4):457-461. [17]Zhou M,Wang D,Kudinha T,et al.Comparative Evaluation of Four Phenotypic Methods for Detection of Class A and B Carbapenemase-Producing Enterobacteriaceae in China[J].J Clin Microbiol,2018,56(8):e00395-18. [18]Jing X,Zhou H,Min X,et al.The Simplified Carbapenem Inactivation Method (sCIM) for Simple and Accurate Detection of Carbapenemase-Producing Gram-Negative Bacilli[J].Front Microbiol,2018(9):2391. [19]Glupczynski Y,Evrard S,Ote I,et al.Evaluation of two new commercial immunochromatographic assays for the rapid detection of OXA-48 and KPC carbapenemases from cultured bacteria[J].J Antimicrob Chemother,2016,71(5):1217-1222. [20]Chen L,Mediavilla JR,Endimiani A,et al.Multiplex real-time PCR assay for detection and classification of Klebsiella pneumoniae carbapenemase gene (bla KPC) variants[J].J Clin Microbiol,2011,49(2):579-585. [21]Subirats J,Royo E,Balcázar JL,et al.Real-time PCR assays for the detection and quantification of carbapenemase genes (bla KPC, bla NDM, and bla OXA-48) in environmental samples[J].Environ Sci Pollut Res Int,2017,24(7):6710-6714. [22]Weiβ D,Engelmann I,Braun SD,et al.A multiplex real-time PCR for the direct,fast,economic and simultaneous detection of the carbapenemase genes blaKPC ,blaNDM ,blaVIM and blaOXA-48[J].J Microbiol Methods,2017(142):20-26. [23]Mathers AJ,Stoesser N,Sheppard AE,et al.Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at a single institution: insights into endemicity from wholegenome sequencing[J].Antimicrob Agents Chemother,2015(59):1656-1663. [24]Burckhardt I,Zimmermann S.Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours[J].J Clin Microbiol,2011,49(9):3321-3324. [25]Hrabák J,Walková R,Studentová V,et al.Carbapenemase activity detection by matrix-assisted laser desorption ionization-time of flight mass spectrometry[J].J Clin Microbiol,2011,49(9):3222-3227. [26]Hrabák J,Studentová V,Walková R,et al.Detection of NDM-1,VIM-1,KPC,OXA-48,and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry[J].J Clin Microbiol,2012,50(7):2441-2443. [27]Youn JH,Drake SK,Weingarten RA,et al.Clinical Performance of a Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Method for Detection of Certain blaKPC-Containing Plasmids[J].J Clin Microbiol,2016,54(1):35-42.

相似文献/References:

[1]尹利娟,杨振华,杨乐园.2017年我院肠杆菌科细菌临床分布及耐药性分析[J].医学信息,2019,(01):136.[doi:10.3969/j.issn.1006-1959.2019.01.042]
 YIN Li-juan,YANG Zhen-hua,YANG Le-yuan.Analysis of Clinical Distribution and Drug Resistance of Enterobacteriaceae in Our Hospital in 2017[J].Medical Information,2019,(19):136.[doi:10.3969/j.issn.1006-1959.2019.01.042]

更新日期/Last Update: 2019-10-01