[1]霍晓奎,王长远.高效液相色谱-串联质谱法测定人血浆盐酸伪麻黄碱浓度[J].医学信息,2019,32(19):163-164173.[doi:10.3969/j.issn.1006-1959.2019.19.054]
　HUO Xiao-kui,WANG Chang-yuan.Determination of Pseudoephedrine Hydrochloride in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J].Journal of Medical Information,2019,32(19):163-164173.[doi:10.3969/j.issn.1006-1959.2019.19.054]
点击复制

高效液相色谱-串联质谱法测定人血浆盐酸伪麻黄碱浓度()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年19期
页码:
163-164173
栏目:
诊疗技术
出版日期:
2019-10-01

文章信息/Info

Title:
Determination of Pseudoephedrine Hydrochloride in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry
文章编号:
1006-1959（2019）19-0163-03
作者:
霍晓奎; 王长远
（大连医科大学药学院，辽宁 大连 116044）
Author(s):
HUO Xiao-kui; WANG Chang-yuan
(School of Pharmacy,Dalian Medical University,Dalian 116044,Liaoning,China)
关键词:
伪麻黄碱; LC-MS/MS; 血药浓度; 药代动力学
Keywords:
Pseudoephedrine; LC-MS/MS; Blood concentration; Pharmacokinetics
分类号:
R969.3
DOI:
10.3969/j.issn.1006-1959.2019.19.054
文献标志码:
A
摘要:
目的 建立一种高效液相色谱-质谱联用技术（LC-MS/MS）测定人血浆盐酸伪麻黄碱浓度的方法。方法 血浆样品经液液萃取后，以甲醇∶水∶甲酸（60∶40∶1，V/V/V）为流动相，流速0.35 ml/min，采用Cap cell pack C18色谱柱分离，150 mm×2.1 mm， 5 μm（USA）；柱温20℃，选择离子喷雾离子化源串联质谱，通过正离子方式检测；选择反应监测（MRM）方式转化m/z=166.3/148.2（盐酸伪麻黄碱）和m/z= 152.2/134.15（内标苯丙醇胺）进行定量分析，用内标法测定含量。结果 伪麻黄碱浓度分别在5.0~400.0 ng/ml的范围内线性良好，R=0.9962，最低检测限为1.0 g/ml。结论 本方法操作简便、快速，结果准确，可用于该药物的含量测定，同时也可为临床药动学研究提供一定的参考。
Abstract:
Objective To establish a high performance liquid chromatography-mass spectrometry (LC-MS/MS) method for the determination of pseudoephedrine hydrochloride in human plasma.Methods After the liquid sample was extracted by liquid-liquid extraction, the mobile phase was methanol:water:formic acid (60∶40∶1, V/V/V), the flow rate was 0.35 ml/min, and separated by Cap cell pack C18 column, 150 mm×2.1mm,5 μm (USA); column temperature 20°C, selective ion spray ionization source tandem mass spectrometry, positive ion detection; selective reaction monitoring (MRM) conversion m/z=166.3/148.2 (pseudoephedrine hydrochloride) and m/z=152.2/134.15 (internal standard phenylpropanolamine) for quantitative analysis, content determined by internal standard method.Results The pseudoephedrine concentration was linear in the range of 5.0~400.0 ng/ml,R=0.9962, and the minimum detection limit was 1.0 g/ml.Conclusion The method is simple, rapid and accurate, and can be used for the determination of the drug content. It can also provide a reference for clinical pharmacokinetic studies.

参考文献/References:

[1]木合塔尔·吐尔洪,楚刚辉,木尼热·阿不都克里木.新疆戈壁野生木贼麻黄中麻黄碱和盐酸伪麻黄碱含量的测定[J].南开大学学报（自然科学版）,2016,49(5):74-78. [2]黄韵然,王晓玲,谢志宏,等.盐酸伪麻黄碱/盐酸非索非那定复方缓释胶囊的研究.Ⅱ.质量研究[J].中国医药工业杂志,2016,47(4):448-453. [3]曹燕妮,李传勋,吕佳,等.HPLC法测定咳喘宁口服液中盐酸麻黄碱和盐酸伪麻黄碱的含量[J].中国民族民间医药,2017(21):22-25. [4]邱燕.HPLC法测定防风通圣颗粒中盐酸麻黄碱和盐酸伪麻黄碱含量[J].海峡药学,2018(1):75-77. [5]王博,孟乡,孟繁蕴,等.胶束毛细管电泳法同时测定小青龙颗粒中的盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱与芍药苷含量[J].中国药房,2019(1):45-49. [6]国家药典委员会编.中华人民共和国药典2015年版一部[M].北京:中国医药科技出版社,2015:890-892． [7]巩长芹,周利章.反相高效液相色谱法测定黄龙止咳颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量[J].中国医院用药评价与分析,2014(3):225-227.

更新日期/Last Update: 2019-10-01