[1]杨雅娟,李 静,朱 琳,等.食管鳞癌组织中miRNAs差异表达谱的筛选及研究[J].医学信息,2019,(20):63-66.[doi:10.3969/j.issn.1006-1959.2019.20.018]
 YANG Ya-juan,LI Jing,ZHU Lin,et al.Screening and Study of Differential Expression Profiles of miRNAs in Esophageal Squamous Cell Carcinoma[J].Medical Information,2019,(20):63-66.[doi:10.3969/j.issn.1006-1959.2019.20.018]
点击复制

食管鳞癌组织中miRNAs差异表达谱的筛选及研究()
分享到:

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期数:
2019年20期
页码:
63-66
栏目:
论著
出版日期:
2019-10-15

文章信息/Info

Title:
Screening and Study of Differential Expression Profiles of miRNAs in Esophageal Squamous Cell Carcinoma
文章编号:
1006-1959(2019)20-0063-04
作者:
杨雅娟1李 静1朱 琳2石俊杰3乔冠恩1
(邯郸市第一医院消化科1,全科2,胸外科3,河北 邯郸 056002)
Author(s):
YANG Ya-juan1LI Jing1ZHU Lin2SHI Jun-jie3QIAO Guan-en1
(Department of Gastroenterology1,Department of General Surgery2,Department of Thoracic Surgery3,the First Hospital of Handan City,Handan 56002,Hebei,China)
关键词:
食管鳞癌miRNAs芯片microRNAs
Keywords:
Esophageal squamous cell carcinomamiRNAs chipmicroRNAs
分类号:
R735.1
DOI:
10.3969/j.issn.1006-1959.2019.20.018
文献标志码:
A
摘要:
目的 筛选并鉴定在食管鳞癌(ESCC)及癌旁组织中miRNAs的差异表达,为进一步阐明其在ESCC发病机制中的作用奠定基础。方法 选取在邯郸市第一医院行食管癌切除术患者新鲜的鳞癌组织及癌旁正常组织,各3例,抽提总RNA,利用miRNAs芯片筛选其中差异表达的miRNAs,并对miR-106b-3p通过进一步RT-qPCR 技术进行验证。结果 miRNAs芯片从配对组织中共筛检出62个差异表达的miRNAs,其中41个miRNAs表达上调,21个miRNAs表达下调,鳞癌组织与癌旁正常组织中差异表达的miRNAs比较,差异有统计学意义(P<0.05)。miR-106b-3p在ESCC组织中的表达高于癌旁组织,差异有统计学意义(P<0.05),与芯片结果一致。结论 食管鳞癌组织中miRNAs的差异表达为进一步研究miRNAs在ESCC发病中的作用奠定了基础;miR-106b-3p的高表达可能与ESCC的发生、发展有关。
Abstract:
Objective To screen and identify differential expression of miRNAs in esophageal squamous cell carcinoma (ESCC) and adjacent tissues, and to lay the foundation for further elucidation of its role in the pathogenesis of ESCC.Methods Fresh squamous cell carcinoma and adjacent normal tissues were obtained from patients with esophageal cancer resection in the First Hospital of Handan City, 3 cases were extracted, total RNA was extracted, and miRNAs were used to screen differentially expressed miRNAs, and miR-106b-3p was used. Verification by further RT-qPCR technology.Results A total of 62 differentially expressed miRNAs were screened from the paired tissues by miRNAs. Among them, 41 miRNAs were up-regulated, 21 miRNAs were down-regulated, and miRNAs were differentially expressed in squamous cell carcinoma and adjacent normal tissues,the difference was statistically significant (P<0.05). The expression of miR-106b-3p in ESCC tissues was higher than that in adjacent tissues,the difference was statistically significant (P<0.05), which was consistent with the chip results.Conclusion The differential expression of miRNAs in esophageal squamous cell carcinoma lays a foundation for further study of the role of miRNAs in the pathogenesis of ESCC. The high expression of miR-106b-3p may be related to the occurrence and development of ESCC.

参考文献/References:

[1]Liu B,Li J,Cairns MJ.Identifying miRNAs,targets and functions[J].Briefings in Bioinformatics,2014,15(1):1-19. [2]Ameres SL,Zamore PD.Diversifying microRNA sequence and function[J].Nature Reviews Molecular Cell Biology,2013,14(8):475-488. [3]Orom UA,Nielsen FC,Lund AH.MicroRNA-10a binds the 5′MUTR of ribosomal protein mRNAs and enhances their translation[J].Molecular cell,2008,30(4):460-471. [4]Qin W,Shi Y,Zhao B,et al.miR-24 Regulates Apoptosis by Targeting the Open Reading Frame (ORF)Region of FAF1 in Cancer Cells[J].PLoS One,2010,5(2):e9429. [5]Singh NK.miRNAs target databases:developmental methods and target identification techniques with functional annotations[J].Cellular and Molecular Life Sciences,2017,74(12):2239-2261. [6]Fassan M,Baffa R,Palazzo JP,et al.MicroRNA expression profiling of male breast cancer[J].Breast Cancer Research,2009,11(4):R58. [7]Syed P,Gidwani K,Kekki H,et al.Role of lectin microarrays in cancer diagnosis[J].Proteomics,2016,16(8):1257-1265. [8]Hu B,Niu X,Cheng L,et al.Discovering cancer biomarkers from clinical samples by protein microarrays[J].Proteomics Clin Appl,2015,9(1-2):98-110. [9]Willier S,Butt E,Grunewald TGP.Lysophosphatidic acid(LPA)signalling in cell migration and cancer invasion:A focussed review and analysis of LPA receptor gene expression on the basis of more than 1700 cancer microarrays[J].Biology of the Cell,2013,105(8):317-333. [10]周萍.食管鳞状细胞癌转移相关miRNa-612调控P53的功能和机制研究[D].山东大学,2017. [11]姚丽.miR-1在食管鳞状细胞癌发生中的作用及其机制的初步研究[D].第四军医大学,2015. [12]Okumura T,Kojima H,Miwa T,et al.The expression of microRNA 574-3p as a predictor of postoperative outcome in patients with esophageal squamous cell carcinoma[J].World Journal of Surgical Oncology,2016,14(1):228.

更新日期/Last Update: 2019-10-15