[1]张小敏,王 洁,王淑静,等.DLPME-HPLC法同时测定人血清中维生素A和维生素E含量[J].医学信息,2019,32(20):161-163.[doi:10.3969/j.issn.1006-1959.2019.20.051]
　ZHANG Xiao-min,WANG Jie,WANG Shu-jing,et al.Simultaneous Determination of Vitamin A and Vitamin E in Human Serum by DLPME-HPLC[J].Journal of Medical Information,2019,32(20):161-163.[doi:10.3969/j.issn.1006-1959.2019.20.051]
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DLPME-HPLC法同时测定人血清中维生素A和维生素E含量()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年20期
页码:
161-163
栏目:
诊疗技术
出版日期:
2019-10-15

文章信息/Info

Title:
Simultaneous Determination of Vitamin A and Vitamin E in Human Serum by DLPME-HPLC
文章编号:
1006-1959（2019）20-0161-03
作者:
张小敏; 王 洁; 王淑静; 朱佳佳
（宁夏医科大学医学科学技术研究中心，宁夏 银川 750004）
Author(s):
ZHANG Xiao-min; WANG Jie; WANG Shu-jing; ZHU Jia-jia
(Ningxia Medical University Medical Science and Technology Research Center,Yinchuan 750004,Ningxia,China)
关键词:
分散液相微萃取; 高效液相色谱; 人血清; 维生素A; 维生素E
Keywords:
Dispersive liquid phase microextraction; High performance liquid chromatography; Human serum; Vitamin A; Vitamin E
分类号:
R151.2
DOI:
10.3969/j.issn.1006-1959.2019.20.051
文献标志码:
A
摘要:
目的 建立分散液相微萃取（DLPME）-高效液相色谱（HPLC）同时测定人血清中维生素A、E含量的方法。方法 2018年5月随机抽取人血液样本50例，经DLPME技术处理后，采用HPLC对人血清中维生素A、E含量进行检测，使用标准曲线法进行定量分析。结果 维生素A标准曲线方程为y=13225x+410.03，相关系数为0.9998，检出限为0.042 μg/ml；维生素E标准曲线方程为y=1383.9x-458.27，相关系数为0.9997，检出限为0.096 μg/ml。维生素A和维生素E的加标回收率分别为90.12%~103.70%和81.09%~92.21%，含量范围分别为（0.62±0.04）μg/ml和（8.41±0.43）μg/ml。结论 DLPME-HPLC法操作简便，节约试剂，对样本富集效率高，通过该方法的研究，对微量血清中维生素A和维生素E的测定具很好的的应用价值。
Abstract:
Objective To establish a method for simultaneous determination of vitamin A and E in human serum by dispersive liquid phase microextraction (DLPME)-high performance liquid chromatography (HPLC). Methods In May 2018, 50 human blood samples were randomly selected. After treatment with DLPME technique, the contents of vitamin A and E in human serum were detected by HPLC and quantified by standard curve method.Results The standard curve of vitamin A was y=13225x+410.03, the correlation coefficient was 0.9998, the detection limit was 0.042 μg/ml; the standard curve of vitamin E was y=1383.9x-458.27, the correlation coefficient was 0.9997, and the detection limit was 0.096 μg/ml. The recoveries of vitamin A and vitamin E were 90.12%~103.70% and 81.09%~92.21%, respectively, and the contents ranged from (0.62±0.04)μg/ml and (8.41±0.43)μg/ml.Conclusion DLPME-HPLC method is easy to operate, saves reagents, and has high efficiency of sample enrichment. The research of this method has a good application value for the determination of vitamin A and vitamin E in trace serum.

参考文献/References:

[1]石庆平,王铁华,张浩,等.反相高效液相色谱法检查复方维生素注射液中的有关物质[J].海军医学杂志,2014,5(2):143-147. [2]刘信嘉,林森煜,李秀英,等.正相高效液相色谱法同时测定奶粉中维生素A醋酸酯、维生素A棕榈酸酯、维生素E醋酸酯及α-生育酚[J].广东化工,2015,42(5):126-127. [3]贾妍,战思恩,翟燕红,等.液相色谱串联质谱法测定血清维生素A、E含量[J].标记免疫分析与临床,2018,25(4):574-579. [4]范菲,杨会鸽,周娜,等.HPLC法同时测定多维元素片中维生素A和维生素E的含量[J].西北药学杂志,2016,31(6):590-592. [5]张航.离子液体液相微萃取有机紫外线吸收剂的研究[D].云南师范大学,2016.

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更新日期/Last Update: 2019-10-15