[1]王丹丹.UPLC测定健脾益肺口服液中白术内酯Ⅰ和白术内酯Ⅲ的含量[J].医学信息,2019,32(21):159-160163.[doi:10.3969/j.issn.1006-1959.2019.21.053]
　WANG Dan-dan.Determination of Atractylenolide I and Atractylenolide III in Jianpi Yifei Oral Liquid by UPLC[J].Journal of Medical Information,2019,32(21):159-160163.[doi:10.3969/j.issn.1006-1959.2019.21.053]
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UPLC测定健脾益肺口服液中白术内酯Ⅰ和白术内酯Ⅲ的含量()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年21期
页码:
159-160163
栏目:
诊疗技术
出版日期:
2019-11-01

文章信息/Info

Title:
Determination of Atractylenolide I and Atractylenolide III in Jianpi Yifei Oral Liquid by UPLC
文章编号:
1006-1959（2019）21-0159-03
作者:
王丹丹
（南京市中医药大学附属南京市中医院药学部，江苏 南京 210001）
Author(s):
WANG Dan-dan
(Department of Pharmacy,Nanjing Traditional Chinese Medicine Hospital,Nanjing University of Traditional Chinese Medicine,Nanjing 210001,Jiangsu,China)
关键词:
UPLC; 白术内酯Ⅰ; 白术内酯Ⅲ
Keywords:
UPLC; Atractylenolide I; Atractylenolide III
分类号:
R284.1
DOI:
10.3969/j.issn.1006-1959.2019.21.053
文献标志码:
A
摘要:
目的 使用UPLC测定健脾益肺口服液中白术内酯Ⅰ、白术内酯Ⅲ的含量。方法 采用Waters H-class 超高效液相色谱仪测定白术内酯Ⅰ、白术内酯Ⅲ的含量，色谱柱：Waters BEH C18（50 mm×2.1 mm，1.7 μm），柱温：30℃，进样量：2 μl，检测波长：220 nm，流动相：乙腈（A）-水（B），梯度洗脱，流速：0.2 ml/min。结果 白术内酯Ⅰ线性方程为y=0.0528x+0.0241（r=0.9998），线性范围2~160 μg/ml；白术内酯Ⅲ的线性方程为y=0.0786x（r=0.9998），线性范围3~140 μg/ml；白术内酯Ⅰ及白术内酯Ⅲ的精密度、重复性、稳定性、加样回收率均符合要求。结论 初步建立了健脾益肺口服液中白术内酯Ⅰ、白术内酯Ⅲ含量测定的高效、迅速的检测方法，为下一步质量标准的提高奠定基础。
Abstract:
Objective To determine the content of atractylenolide I and atractylenolide III in Jianpi Yifei Oral Liquid by UPLC. Methods The contents of atractylenolide I and atractylenolide III were determined by Waters H-class ultra performance liquid chromatography. Column: Waters BEH C18 (50 mm × 2.1 mm, 1.7 μm), column temperature: 30°C, injection Amount: 2 μl, detection wavelength: 220 nm, mobile phase: acetonitrile (A)-water (B), gradient elution, flow rate: 0.2 ml/min. Results The linear equation of atractylenolide I was y=0.0528x+0.0241 (r=0.9998), the linear range was 2~160 μg/ml; the linear equation of atractyl lactone III was y=0.0786x (r=0.9998), linear range 3 ~140 μg/ml; the precision, repeatability, stability and recovery rate of atractylenolide I and atractylenolide III meet the requirements. Conclusion The high-efficiency and rapid detection method for the determination of atractylenolide I and atractylenolide III in Jianpi Yifei Oral Liquid was established, which laid a foundation for the improvement of quality standards.

参考文献/References:

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更新日期/Last Update: 2019-11-01