[1]梁子聪,王 恒,胡先运,等.鹿角壮骨胶囊对膝骨关节炎兔血清及软骨IL-6、MMP-3的影响[J].医学信息,2020,33(01):61-63.[doi:10.3969/j.issn.1006-1959.2020.01.020]
　LIANG Zi-cong,WANG Heng,HU Xian-yun,et al.Effects of Lujiao Zhuanggu Capsule on Serum and Cartilage IL-6 and MMP-3 in Rabbits with Knee Osteoarthritis[J].Medical Information,2020,33(01):61-63.[doi:10.3969/j.issn.1006-1959.2020.01.020]
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鹿角壮骨胶囊对膝骨关节炎兔血清及软骨IL-6、MMP-3的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年01期
页码:
61-63
栏目:
论著
出版日期:
2020-01-01

文章信息/Info

Title:
Effects of Lujiao Zhuanggu Capsule on Serum and Cartilage IL-6 and MMP-3 in Rabbits with Knee Osteoarthritis
文章编号:
1006-1959（2020）01-0061-03
作者:
梁子聪1; 2; 3; 王 恒1; 胡先运1; 江 勇2; 胡建山2; 3; 陆瑞娜4; 刘运麟4
（1.黔南民族医学高等专科学校基础医学部，贵州 都匀 558000；2.黔南州中医医院骨科，贵州 都匀 558000；3.三都爱善医院中医科，贵州 三都 558100；4.黔南州人民医院/黔南医专第一附属医院超声科，贵州 都匀 558000）
Author(s):
LIANG Zi-cong1; 2; 3; WANG Heng1; HU Xian-yun1; JIANG Yong2; HU Jian-shan2; 3; LU Rui-na4; LIU Yun-lin4
(1.Department of Basic Medicine,Qiannan Medical College for Nationalities,Duyun 558000,Guizhou,China;2.Department of Orthopaedics,Qiannanzhou Traditional Chinese Medicine Hospital,Duyun 558000,Guizhou,China;3.Department of Traditional Chinese Medicine,San
关键词:
膝骨关节炎; 鹿角壮骨胶囊; 血清; 白介素-6; 金属基质蛋白酶; 关节软骨
Keywords:
Knee osteoarthritis; Lujiao Zhuanggu Capsule; Serum; Interleukin-6; Metal matrix protease;  Articular cartilage
分类号:
R684.3
DOI:
10.3969/j.issn.1006-1959.2020.01.020
文献标志码:
A
摘要:
目的 探讨鹿角壮骨胶囊对膝骨关节炎兔血清及软骨IL-6、MMP-3的影响。方法 采用木瓜蛋白酶右侧膝关节腔内注射诱导建立兔膝骨关节炎模型，将24只长耳大白兔随机分为正常组、模型组、治疗组，每组8只。正常组和模型组给予蒸馏水灌胃，治疗组给予鹿角壮骨胶囊灌胃，治疗4周后采用ELISA法检测血清IL-6、MMP-3的含量，免疫组化法检测各组兔膝关节软骨IL-6、MMP-3的表达。结果 模型组、治疗组血清IL-6、MMP-3水平高于正常组，治疗组血清MMP-3水平低于模型组，差异有统计学意义（P＜0.05）。模型组和治疗组膝关节软骨IL-6、MMP-3表达高于正常组，治疗组IL-6、MMP-3低于模型组，差异有统计学意义（P＜0.05）。结论 鹿角壮骨胶囊可通过抑制IL-6、MMP-3等致炎因子的表达起到防治膝骨关节炎的作用。
Abstract:
Objective To investigate the effects of Lujiao Zhuanggu Capsule on serum and cartilage IL-6 and MMP-3 in rabbits with knee osteoarthritis. Methods The rabbit knee osteoarthritis model was induced by papain injection into the right knee joint cavity. 24 long-eared white rabbits were randomly divided into normal group, model group and treatment group, 8 in each group. The normal group and the model group were administrated with distilled water. The treatment group was administrated with Lujiao Zhuanggu Capsule. After 4 weeks of treatment, the levels of IL-6 and MMP-3 in serum were detected by ELISA. Expression of IL-6 and MMP-3. Results The serum levels of IL-6 and MMP-3 in the model group and the treatment group were higher than those in the normal group,the difference was statistically significant (P<0.05); the serum MMP-3 levels in the treatment group were lower than the model group,the difference was statistically significant (P<0.05); the expression of IL-6 and MMP-3 in rabbit knee joint cartilage was higher in the model group and the treatment group than in the normal group,the difference was statistically significant(P<0.05); IL-6 and MMP-3 in the treatment group were lower than the model group, the difference was statistically significant(P<0.05).Conclusion Lujiao Zhuanggu Capsule can prevent knee osteoarthritis by inhibiting the expression of IL-6, MMP-3 and other inflammatory factors.

参考文献/References:

[1]Lane NE,Shidara K,Wise BL.Osteoarthritis year in review 2016：clinical[J].Osteoarthritis and Cartilage,2017,25(2):209-215. [2]谷艳超,刘世清,夏韶强,等.骨关节炎发病机制和治疗的研究进展[J].中国骨与关节杂志,2016,5(10):770-774. [3]何方舟,张伟滨.非甾体类抗炎药对中国骨关节炎患者疗效及胃肠道不良反应的Meta分析[J].中华关节外科杂志(电子版),2018,12(1):69-76. [4]陈主初,吴端生.实验动物动物学[M].第4版.长沙:湖南科技出版社,2001:321. [5]葛伟韬,高云,刘珍珠,等.膝骨关节炎中医病名辨识[J].中医杂志,2016,57(23):1989-1992. [6]李西海,刘献祥.骨关节炎的核心病机-本痿标痹[J].中医杂志,2014,55(14):1248-1249,1252. [7]Blom AB,Lent P,Libregts S,et al.Crucial role of macrophages in matrix metalloproteinase-mediated cartilage destruction during experimental osteoarthritis:involvement of matrix metalloproteinase 3[J].Arthritis& Rheumatology,2010,56(1):147-157. [8]苏军,陆永刚,胡建山,等.鹿角壮骨胶囊治疗膝骨性关节炎的疗效观察[J].中药药理与临床,2015,31(2):169-170. [9]金连峰.单味中药骨碎补对兔膝骨关节炎软骨细胞凋亡作用的实验研究[J].中华中医药学刊,2013,31(7):1699-1703. [10]金连峰,韩煜.骨碎补对兔膝骨关节炎关节软骨细胞凋亡相关因子作用实验研究[J].辽宁中医药大学学报,2013,15(11):42-46. [11]李冬方,顾宁,黄伟.黄芪多糖对THP-1巨噬细胞HSP60诱导下IL-6、TNF-α表达的影响[J].时珍国医国药,2015,26(12):2830-2832. [12]罗华丽.黄芪甲苷对骨关节炎保护作用的定量蛋白质组学研究[D].重庆医科大学,2016. [13]袁立霞.当归拈痛汤及其拆方对类风湿性关节炎大鼠滑膜组织MMP-3调节作用的实验研究[J].时珍国医国药,2013,24(6):1313-1314. [12]林嘉辉,陈炳艺,龙美兵,等.龟甲胶和鹿角胶含药血清对豚鼠骨关节炎软骨细胞JNK及p38 MAPK基因表达的影响[J].中国中医骨伤科杂志,2016,24(10):1-4. [13]陈炳艺,陈泽华,林嘉辉,等.龟甲胶、鹿角胶调控MKK基因表达促进豚鼠OA软骨细胞增殖的研究[J].中国骨质疏松杂志,2016,22(7):805-808.

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更新日期/Last Update: 2020-01-01