[1]付满玲,黄雨佳,刘珉甬,等.山楂代用茶对大鼠经口急性毒性实验[J].医学信息,2020,33(01):77-80.[doi:10.3969/j.issn.1006-1959.2020.01.024]
　FU Man-ling,HUANG Yu-jia,LIU Min-yong,et al.Experimental on Oral Acute Toxicity of Hawthorn Substitute Tea on Rats[J].Medical Information,2020,33(01):77-80.[doi:10.3969/j.issn.1006-1959.2020.01.024]
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山楂代用茶对大鼠经口急性毒性实验()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年01期
页码:
77-80
栏目:
论著
出版日期:
2020-01-01

文章信息/Info

Title:
Experimental on Oral Acute Toxicity of Hawthorn Substitute Tea on Rats
文章编号:
1006-1959（2020）01-0077-04
作者:
付满玲1; 黄雨佳2; 刘珉甬3; 郭辉梦4
（1.西南医科大学护理器械研发实验室，四川 泸州 646000；2.四川护理职业学院康复技术系，四川 成都 610000；3.泸州欣康生物科技有限公司，四川 泸州 646000；4.西南医科大学附属医院医学实验中心，四川 泸州 646000）
Author(s):
FU Man-ling1; HUANG Yu-jia2; LIU Min-yong3; GUO Hui-meng4
(1.Nursing Device Research and Development Laboratory,Southwest Medical University,Luzhou 646000,Sichuan,China;2.Department of Rehabilitation Technology,Sichuan Vocational College of Nursing,Chengdu 610000,Sichuan,China;3.Luzhou Xinkang Biotechnology Co.,
关键词:
山楂代用茶; 高脂血症; 经口急性毒性实验
Keywords:
Hawthorn substitute tea; Hyperlipidemia; Oral acute toxicity test
分类号:
R961.1
DOI:
10.3969/j.issn.1006-1959.2020.01.024
文献标志码:
A
摘要:
目的 通过大鼠经口急性毒性动物实验评价山楂代用茶的安全性。方法 选择20只SD大鼠，随机分为实验组和对照组，各10只（雌雄各半），实验组给予山楂代用茶最大浓度0.3 g/ml、3 ml/只、2次/d灌胃，对照组给予生理盐水3 ml/只、2次/d灌胃，连续观察7 d。比较两组大鼠一般情况、有无死亡、血液生化、脏器系数及病理组织。结果 ①一般情况：两组大鼠排尿、粪便、活动度均正常，两组第1、3、5、7 天进食量及体重比较，差异均无统计学意义（P＞0.05）；②两组均无大鼠死亡；③血液生化指标：两组ALT、AST、AST/ALT、TP、ALB、GLO、TBIL、ALP、Urea、UA及Crea比较，差异均无统计学意义（P＞0.05）；④大体解剖：解剖后肉眼观察到两组心、肝、肾、肺体积、颜色、质地均无异常。脏器系数结果显示，两组雌性、雄性大鼠心、肝、脾、肺、肾系数比较，差异均无统计学意义（P＞0.05），且皆符合相应月龄大鼠的脏器系数；⑤病理观察：两组肝脏均正常，仅见肝细胞中央汇管区有少量的炎细胞浸润；两组肾小管结构正常，皮髓质分界清，肾小管上皮、淋巴细胞未见病理性改变。结论 山楂代用茶对大鼠的日累积剂量为临床成人（按 60 kg计算）拟推荐日用剂量的108倍，在此剂量下大鼠一般情况、血液生化指标、肝脏、肾脏的病理组织均无毒性反应，提示山楂代用茶对小鼠无急性毒性作用。
Abstract:
Objective To evaluate the safety of hawthorn substitute tea by oral acute toxicity animal experiments in rats. Methods 20 SD rats were selected and randomly divided into an experimental group and a control group, 10 rats each (male and female half). The experimental group was given a hawthorn substitute tea with a maximum concentration of 0.3 g/ml, 3 ml/one, 2 times/d.The control group was administrated with saline 3 ml/one, 2 times/d gavage,7 d continuous observation.The general conditions, death, blood biochemistry, organ coefficients and pathological tissues of the two groups of rats were compared.Results ①General situation: The urine, feces, and activity of the two groups of rats were normal. There was no significant difference in the food intake and weight of the two groups on 1st, 3rd, 5th, and 7th days(P>0.05)；②No rats died in both groups; ③Blood biochemical indicators: there was no significant difference in comparison of ALT, AST, AST/ALT, TP, ALB, GLO, TBIL, ALP, Urea, UA and Crea between the two groups (P>0.05); ④Gross anatomy: After the dissection, the heart, liver, kidney, lung volume, color, and texture of the two groups were not abnormal. The results of organ coefficients showed that there was no significant difference in the coefficients of heart, liver, spleen, lung, and kidney of female and male rats between the two groups (P>0.05), and all were consistent with the organ coefficients of the corresponding month-old rats;⑤Pathological observation: The livers of the two groups were normal. Only a small amount of inflammatory cells infiltrated in the central manifold area of the hepatocytes were observed. The renal tubular structure was normal, the cortex and medulla were clearly demarcated, and the renal tubular epithelium and lymphocytes were not changed.Conclusion The daily cumulative dose of Hawthorn substitute tea for rats is 108 times the recommended daily dose for clinical adults (calculated based on 60 kg). At this dose, the general condition of rats, blood biochemical indicators, pathological tissues of liver and kidney were all non-toxic, suggesting that the hawthorn substitute tea has no acute toxicity on mice.

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更新日期/Last Update: 2020-01-01