[1]王东梅,张展英.小细胞肺癌中信号通路及蛋白相互作用网络分析[J].医学信息,2020,33(01):81-84.[doi:10.3969/j.issn.1006-1959.2020.01.025]
　WANG Dong-mei,ZHANG Zhan-ying.Analysis of Signal Pathways and Protein Interaction Networks in Small Cell Lung Cancer[J].Medical Information,2020,33(01):81-84.[doi:10.3969/j.issn.1006-1959.2020.01.025]
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小细胞肺癌中信号通路及蛋白相互作用网络分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年01期
页码:
81-84
栏目:
论著
出版日期:
2020-01-01

文章信息/Info

Title:
Analysis of Signal Pathways and Protein Interaction Networks in Small Cell Lung Cancer
文章编号:
1006-1959（2020）01-0081-04
作者:
王东梅1; 张展英2
（1.上海其明信息技术有限公司，上海 200000；2.美艾利尔<上海>诊断产品有限公司，上海 200000）
Author(s):
WANG Dong-mei1; ZHANG Zhan-ying2
(1.Shanghai Qiming Information Technology Co.,Ltd.,Shanghai 200000,China;2.MayerDiagnostic Products Co.,Ltd.,Shanghai 200000,China)
关键词:
小细胞肺癌; 基因突变; 信号通路; 蛋白相互作用网络
Keywords:
Small cell lung cancer; Gene mutation; Signaling pathway; Protein interaction network
分类号:
R734.2
DOI:
10.3969/j.issn.1006-1959.2020.01.025
文献标志码:
A
摘要:
目的 分析小细胞肺癌相关的信号通路，构建蛋白相互作用网络，探究小细胞肺癌相关的关键基因。方法 从cBioPortal数据库下载110例小细胞肺癌患者临床和全基因组测序数据，采用R软件分析数据的基本信息，KEGG信号通路富集分析，构建蛋白质相互作用网络。结果 共收集110例小细胞肺癌患者，其中男性多于女性，大部分患者年龄在60岁及以上，且吸烟人数多于戒烟人数，吸烟患者的突变位点数（n=345.4）高于戒烟组（n=299.70），差异有统计学意义（P＜0.05）；信号通路富集显示，发生突变的基因主要与Wnt信号通路、钙粘蛋白信号通路、烟碱乙酰胆碱受体信号通路有关。蛋白相互作用网络显示，与小细胞肺癌相关的基因有10个，其中PIK3CG和ERBB4的度最大。结论 小细胞肺癌与吸烟有关，且吸烟可引起基因突变位点数增多。小细胞肺癌与Wnt信号通路、钙粘蛋白信号通路、烟碱乙酰胆碱受体信号通路有关，与PIK3CG、ERBB4基因关系密切。
Abstract:
Objective To analyze the signaling pathways related to small cell lung cancer, construct a protein interaction network, and explore key genes related to small cell lung cancer. Methods The clinical and whole-genome sequencing data of 110 small cell lung cancer patients were downloaded from the cBioPortal database. The basic information of the data was analyzed using R software, and the KEGG signal pathway enrichment analysis was performed to construct a protein interaction network. Results A total of 110 small cell lung cancer patients were collected, including more men than women, most of whom were aged 60 years and over, and the number of smokers was more than the number of people who quit,the number of mutation sites (N=345.4) in smoking patients was higher than that in the smoking cessation group (N=299.70),the difference was statistically significant(P<0.05). The enrichment of signaling pathways showed that the genes that mutated were mainly related to the Wnt signaling pathway and calcium adhesion Protein signaling pathway and nicotinic acetylcholine receptor signaling pathway are related. The protein interaction network showed that there were 10 genes related to small cell lung cancer, of which PIK3CG and ERBB4 had the highest degree.Conclusion Small cell lung cancer is related to smoking, and smoking can increase the number of gene mutation sites. Small cell lung cancer is related to the Wnt signaling pathway, the cadherin signaling pathway, and the nicotinic acetylcholine receptor signaling pathway, and is closely related to the PIK3CG and ERBB4 genes.

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更新日期/Last Update: 2020-01-01