[1]苏振华.乙肝病毒标记物阳性表型血清标本乙型肝炎病毒HBV-DNA临床检验分析[J].医学信息,2020,33(04):179-180.[doi:10.3969/j.issn.1006-1959.2020.04.061]
 SU Zhen-hua.Clinical Analysis of Hepatitis B Virus HBV-DNA in Serum Specimen with Hepatitis B Virus Markers Positive[J].Medical Information,2020,33(04):179-180.[doi:10.3969/j.issn.1006-1959.2020.04.061]
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乙肝病毒标记物阳性表型血清标本乙型肝炎病毒HBV-DNA临床检验分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年04期
页码:
179-180
栏目:
诊疗技术
出版日期:
2020-02-15

文章信息/Info

Title:
Clinical Analysis of Hepatitis B Virus HBV-DNA in Serum Specimen with Hepatitis B Virus Markers Positive
文章编号:
1006-1959(2020)04-0179-02
作者:
苏振华
(万年县人民医院检验科,江西 万年 335500)
Author(s):
SU Zhen-hua
(Department of Laboratory Medicine,Wannian County People’s Hospital,Wannian 335500,Jiangxi,China)
关键词:
乙型肝炎病毒乙肝病毒标记物表型血清标本
Keywords:
epatitis B virusHBV marker phenotypeSerum specimen
分类号:
R737.33
DOI:
10.3969/j.issn.1006-1959.2020.04.061
文献标志码:
A
摘要:
目的 探讨乙肝病毒标记物阳性表型血清标本在乙型肝炎病毒诊断中的应用。方法 选取2018年5月~2019年5月我院收治的乙肝病毒阳性患者100例作为实验组,另选取同期我院健康体检者100例作为对照组,采用酶联免疫吸附法与PCR荧光定量法对两组乙肝病毒标记物表型血清指标进行检测,比较两组阳性反应组合反应阳性率、HBV-DNA水平。结果 通过酶联免疫吸附法检验,在Presl、HBsAb、HBsAg、HBeAg、HBeAb、HBcAb-IgM、HBcAb-IgG等指标中可表现出11种阳性反应组合,其中HBeAb、HBsAg、HBcAb-IgG阳性反应组合阳性率最高,其次为Presl、HBeAg、HBsAg、HBcAb-IgG阳性反应组合;实验组HBV-DNA水平为(4.70±1.30)×104,对照组HBV-DNA水平为(0.80±0.10)×104,实验组高于对照组,差异有统计学意义(P<0.05)。结论 乙肝病毒标记物表型血清标本检测在乙型肝炎病毒中诊断的价值较高,可为临床诊断提供科学依据。
Abstract:
Objective To investigate the application of hepatitis B virus marker positive phenotype serum samples in the diagnosis of hepatitis B virus.Methods 100 patients with HBV-positive patients admitted to our hospital from May 2018 to May 2019 were selected as the experimental group,and another 100 healthy subjects from our hospital during the same period were selected as the control group.ELISA and PCR fluorescence quantitative methods were used the serum index of HBV marker phenotype was detected in the two groups,and the positive rate of combined positive reactions and HBV-DNA levels were compared between the two groups.Results By enzyme-linked immunosorbent assay,11 positive reaction combinations can be shown in Presl, HBsAb, HBsAg, HBeAg, HBeAb, HBcAb-IgM, HBcAb-IgG and other indicators, among which HBeAb, HBsAg, HBcAb-IgG positive combination positive highest,followed by Presl, HBeAg, HBsAg,and HBcAb-IgG positive reaction combinations;HBV-DNA level in the experimental group was(4.70±1.30)×104,and HBV-DNA level in the control group was(0.80±0.10)×104,the experimental group was higher than the control group,the difference was statistically significant(P<0.05).Conclusion The detection of HBV marker phenotype serum samples is of higher diagnostic value in HBV,and it can provide scientific basis for clinical diagnosis.

参考文献/References:

[1]马梅香.乙肝病毒标记物阳性表型血清标本乙型肝炎病毒(HBV)-DNA临床检验分析的应用效果评价[J].心理月刊,2018,(12):99. [2]王晓玲,苑玉聪,郝金翠,等.乙肝肾合剂辨证加减联合常规西药综合治疗乙型肝炎病毒相关性肾炎的临床观察[J].辽宁中医杂志,2017,44(10):2114-2117. [3]徐茜茜,卓莉,杨悦,等.乙型肝炎病毒相关性肾炎不同年龄组患者的临床病理特点[J].中国病毒病杂志,2016,6(3):206-211. [4]李根瑞,陈涓涓,谢正媛,等.云南省7934例农村育龄人群乙型肝炎病毒标记物血清学筛查检测结果分析[J].中国预防医学杂志,2017,18(1):31-34.

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更新日期/Last Update: 2020-02-15