[1]莫俊贤,冼 磊.miRNA-21及PTEN在肺腺癌组织中的表达及意义[J].医学信息,2020,33(09):77-79.[doi:10.3969/j.issn.1006-1959.2020.09.022]
　MO Jun-xian,XIAN Lei.Expression and Significance of miRNA-21 and PTEN in Lung Adenocarcinoma[J].Medical Information,2020,33(09):77-79.[doi:10.3969/j.issn.1006-1959.2020.09.022]
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miRNA-21及PTEN在肺腺癌组织中的表达及意义()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年09期
页码:
77-79
栏目:
论著
出版日期:
2020-05-01

文章信息/Info

Title:
Expression and Significance of miRNA-21 and PTEN in Lung Adenocarcinoma
文章编号:
1006-1959（2020）09-0077-03
作者:
莫俊贤; 冼 磊
（1.广西医科大学第七附属医院胸心外科，广西 梧州 543000；2.广西医科大学第二附属医院胸心外科，广西 南宁 530007）
Author(s):
MO Jun-xian; XIAN Lei
(1.Department of Thoracic and Cardiovascular Surgery,the Seventh Affiliated Hospital of Guangxi Medical University,Wuzhou 543000,Guangxi,China;2.Department of Thoracic and Cardiovascular Surgery,the Second Affiliated Hospital of Guangxi Medical University,Nanning 530007,Guangxi,China)
关键词:
肺腺癌; miRNA-21; PTEN; 实时荧光定量PCR
Keywords:
Lung adenocarcinoma; miRNA-21; PTEN; Real-time fluorescence quantitative PCR
分类号:
R735.2
DOI:
10.3969/j.issn.1006-1959.2020.09.022
文献标志码:
A
摘要:
目的 观察肺腺癌组织中miRNA-21的表达情况，为miRNA-21成为新的肺腺癌靶点基因提供临床依据。方法 收集自2017年6月~2019年6月广西医科大学第二附属医院共53例肺腺癌患者的癌组织、配对癌旁组织以及12例非癌疾病肺组织。采用实时荧光定量PCR法（qPCR）检测其miRNA-21和PTEN的表达情况，并分析二者的相关性。结果 肺腺癌组织中miRNA-21表达高于癌旁组织和非癌肺组织，其中Ⅲ~Ⅳ期患者中高于Ⅰ~Ⅱ期患者；肺腺癌组织中PTEN低于癌旁组织和非癌肺组织，其中Ⅲ~Ⅳ期患者中低于Ⅰ~Ⅱ期患者，差异有统计学意义（P＜0.05）； miRNA-21和PTEN的表达呈负相关（r=-0.663，P＜0.05）。结论 miRNA-21在肺腺癌组织中抑制了PTEN的表达，miRNA-21极有可能成为肺腺癌的治疗靶点。
Abstract:
Objective To observe the expression of miRNA-21 in lung adenocarcinoma tissues and provide a clinical basis for miRNA-21 to become a new target gene for lung adenocarcinoma.Methods From June 2017 to June 2019, 53 cases of lung adenocarcinoma patients’ cancer tissues, paired paracancerous tissues and 12 cases of non-cancer disease lung tissues were collected from the Second Affiliated Hospital of Guangxi Medical University. Real-time fluorescence quantitative PCR (qPCR) was used to detect the expression of miRNA-21 and PTEN, and to analyze the correlation between the two.Results The expression of miRNA-21 in lung adenocarcinoma tissues was higher than that in paracancerous tissues and non-cancerous lung tissues. Among them, stage III to IV patients were higher than those in stage I to II patients; PTEN in lung adenocarcinoma tissues was lower than that in paracancerous tissues and noncancerous lung tissues.Among them, the patients in stage III to IV were lower than those in stage I to II, the difference was statistically significant (P<0.05); the expression of miRNA-21 and PTEN was negatively correlated(r=-0.663,P<0.05).Conclusion miRNA-21 inhibits the expression of PTEN in lung adenocarcinoma tissues. miRNA-21 is very likely to be a therapeutic target for lung adenocarcinoma.

参考文献/References:

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更新日期/Last Update: 2020-05-01