[1]荀秀彪,王月德.HPLC法测定银菊感冒胶囊中绿原酸的含量[J].医学信息,2020,33(09):155-157.[doi:10.3969/j.issn.1006-1959.2020.09.052]
　XUN Xiu-biao,WANG Yue-de.Determination of Chlorogenic Acid in Yinju Ganmao Capsules by HPLC[J].Medical Information,2020,33(09):155-157.[doi:10.3969/j.issn.1006-1959.2020.09.052]
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HPLC法测定银菊感冒胶囊中绿原酸的含量()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年09期
页码:
155-157
栏目:
诊疗技术
出版日期:
2020-05-01

文章信息/Info

Title:
Determination of Chlorogenic Acid in Yinju Ganmao Capsules by HPLC
文章编号:
1006-1959（2020）09-0155-03
作者:
荀秀彪; 王月德
（云南省曲靖市食品药品检验检测中心，云南 曲靖 655000）
Author(s):
XUN Xiu-biao; WANG Yue-de
(Qujing City Food and Drug Inspection and Testing Center,Yunnan Province,Qujing 655000, Yunnan,China)
关键词:
高效液相色谱法; 银菊感冒胶囊; 绿原酸; 含量测定
Keywords:
High performance liquid chromatography; Yinju Ganmao capsules; Chlorogenic acid; Content determination
分类号:
R725.1
DOI:
10.3969/j.issn.1006-1959.2020.09.052
文献标志码:
A
摘要:
目的 建立银菊感冒胶囊中绿原酸含量测定的高效液相色谱法。方法 采用Hypersil ODS2（4.6 mm×250 mm，5μm）色谱柱；乙腈-0.4%磷酸溶液（9∶91)为流动相；流速：1.0 ml/min；检测波长：327 nm；柱温：30 ℃；进样量：10 μl；理论板数按绿原酸峰计算不低于1000。结果 绿原酸进样量在0.04348~0.39132 μg内线性关系良好，r=0.9997（n=6），平均回收率为98.66%，RSD=0.83%（n=5）。结论 本次建立的方法操作简便快捷，结果可靠，可用于银菊感冒胶囊中绿原酸的含量测定。
Abstract:
Objective To establish a high performance liquid chromatography method for the determination of chlorogenic acid in Yinju Ganmao capsules.Methods Hypersil ODS2 (4.6 mm × 250 mm, 5 μm) chromatographic column; acetonitrile-0.4% phosphoric acid solution (9∶91) as mobile phase; flow rate: 1.0 ml/min; detection wavelength: 327 nm; column temperature: 30 ℃ Injection volume: 10 μl; theoretical plate count is not less than 1000 based on chlorogenic acid peak calculation.Results The chlorogenic acid injection volume was in a linear relationship between 0.04348 and 0.39132 μg, r=0.9997(n=6), the average recovery was 98.66%, and RSD=0.83%(n=5).Conclusion The method established this time is simple and quick, and the results are reliable. It can be used for the determination of chlorogenic acid in Yinju Ganmao capsules.

参考文献/References:

[1]国家药典委员会.中华人民共和国药典：2015年版一部[S].北京:中国医药科技出版社,2015:221,310-311.[2]王锦,马善波,李龙,等.提高咽炎颗粒的质量标准研究[J].西北药学杂志,2018,33(3):51-54.[3]左霞,刘春霞,杨金霞,等.HPLC法测定不同来源不同采收期忍冬藤和金银花中绿原酸含量[J].中国药师,2015(4):158-160.[4]李杨,邓刚,王豫梅,等.不同提取方法对金银花凉茶中绿原酸含量的影响[J].食品工业,2016(9):115-118.[5]屠万倩,刘晓苗,张留记,等.HPLC法同时测定不同产地不同品种菊花中8种成分的含量[J].中药材,2018,41(1):152-155.

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更新日期/Last Update: 2020-05-01