[1]陈真真,蔡 军.osa-miR168a-5p靶向调节人源ADD1及E2F2基因表达的研究[J].医学信息,2020,(11):64-66.[doi:10.3969/j.issn.1006-1959.2020.11.020]
 CHEN Zhen-zhen,CAI Jun.Study on osa-miR168a-5p Targeted Regulation of Human ADD1 and E2F2 Gene Expression[J].Medical Information,2020,(11):64-66.[doi:10.3969/j.issn.1006-1959.2020.11.020]
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osa-miR168a-5p靶向调节人源ADD1及E2F2基因表达的研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期数:
2020年11期
页码:
64-66
栏目:
综述
出版日期:
2020-06-01

文章信息/Info

Title:
Study on osa-miR168a-5p Targeted Regulation of Human ADD1 and E2F2 Gene Expression
文章编号:
1006-1959(2020)11-0064-03
作者:
陈真真蔡 军
(中国医学科学院阜外医院/国家心血管病中心/心血管疾病国家重点实验室/高血压中心,北京100037)
Author(s):
CHEN Zhen-zhenCAI Jun
(Fuwai Hospital of Chinese Academy of Medical Sciences/National Cardiovascular Disease Center/State Key Laboratory of Cardiovascular Diseases/Hypertension Center,Beijing 100037,China)
关键词:
osa-miR168aADD1E2F2靶基因
Keywords:
osa-miR168aADD1E2F2Target gene
分类号:
R3
DOI:
10.3969/j.issn.1006-1959.2020.11.020
文献标志码:
A
摘要:
目的 探究植物osa-miR168a-5p靶向调节人源相关基因表达的影响。方法 采用TargetScan预测osa-miR168a-5p的靶基因,并筛选相关靶基因进行后续验证。构建靶基因mRNA 3’-UTR荧光素报告基因及相应的突变体,采用双荧光素酶报告基因活性分析系统验证osa-miR168a-5p与靶基因的关系。结果 利用Targetscan预测的osa-miR168a-5p靶基因结果显示,ADD1与E2F2mRNA3’-UTR区域均存在osa-miR168a-5p结合位点。双荧光素酶报告基因实验结果显示,使用osa-miR168a-5p mimics内源性增加osa-miR168a-5p能够抑制ADD1及E2F2报告基因的表达,将ADD1及E2F2 mRNA 3’UTR区与osa-miR-168a-5p的结合位点突变后,内源性osa-miR-168a-5p增加所引起的报告基因表达下调的作用消失。结论 osa-miR168a-5p能够直接调节人源ADD1及E2F2基因表达,因此食物中的osa-miR168a进入人体很可能直接靶向人源ADD1及E2F2基因发挥相应的生物学效应。
Abstract:
Objective To explore the effect of plant osa-miR168a-5p on targeted regulation of human-related gene expression.Methods TargetScan was used to predict osa-miR168a-5p target genes, and related target genes were screened for subsequent verification. The target gene mRNA 3’-UTR luciferin reporter gene and corresponding mutants were constructed, and the dual luciferase reporter gene activity analysis system was used to verify the relationship between osa-miR168a-5p and the target gene.Results The results of osa-miR168a-5p target genes predicted by Targetscan showed that both ADD1 and E2F2mRNA 3’-UTR regions had osa-miR168a-5p binding sites. The results of the dual luciferase reporter gene experiment showed that the endogenous increase of osa-miR168a-5p mimics using osa-miR168a-5p can inhibit the expression of ADD1 and E2F2 reporter genes.After mutating the binding site of ADD1 and E2F2 mRNA 3’UTR region to osa-miR-168a-5p, the effect of the endogenous increase of osa-miR-168a-5p on reporter gene expression disappeared.Conclusion osa-miR168a-5p can directly regulate the expression of human ADD1 and E2F2 genes. Therefore, the entry of osa-miR168a in food into the human body is likely to directly target the human ADD1 and E2F2 genes to exert the corresponding biological effects.

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更新日期/Last Update: 1900-01-01