[1]姜 玥,刘宵达,吴素琴.anti-IL-33对类风湿关节炎成纤维样滑膜细胞增殖与凋亡的影响[J].医学信息,2021,34(13):81-83.[doi:10.3969/j.issn.1006-1959.2021.13.020]
　JIANG Yue,LIU Xiao-da,WU Su-qin.The Effect of anti-IL-33 on the Proliferation and Apoptosis of Fibroblast-like Synovial Cells in Rheumatoid Arthritis[J].Medical Information,2021,34(13):81-83.[doi:10.3969/j.issn.1006-1959.2021.13.020]
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anti-IL-33对类风湿关节炎成纤维样滑膜细胞增殖与凋亡的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年13期
页码:
81-83
栏目:
论著
出版日期:
2021-07-01

文章信息/Info

Title:
The Effect of anti-IL-33 on the Proliferation and Apoptosis of Fibroblast-like Synovial Cells in Rheumatoid Arthritis
文章编号:
1006-1959（2021）13-0081-03
作者:
姜 玥; 刘宵达; 吴素琴
（辽宁省基础医学研究所免疫研究室，辽宁 沈阳 110101）
Author(s):
JIANG Yue; LIU Xiao-da; WU Su-qin
(Department of Immunology,Liaoning Institute of Basic Medicine,Shenyang 110101,Liaoning,China)
关键词:
anti-IL-33; 类风湿关节炎成纤维样滑膜细胞; 细胞增殖; 细胞凋亡
Keywords:
anti-IL-33; Rheumatoid arthritis fibroblast-like synovial cells; Cell proliferation;  Apoptosis
分类号:
R593.22
DOI:
10.3969/j.issn.1006-1959.2021.13.020
文献标志码:
A
摘要:
目的 探讨抗白细胞介素-33抗体（anti-IL-33）对类风湿关节炎（RA）成纤维样滑膜细胞（FLS）增殖与凋亡的影响及其在RA疾病中的缓解及治疗作用。方法 培养人FLS，实验分组如下：正常对照组、TNF-α（+）对照组、TNF-α（+）+anti-IL-33（50 ng/ml）组、TNF-α（+）+甲氨蝶呤（1 μg/ml）组。观察RA-FLS的形态结构，并采用CCK-8法测定各组细胞的增殖水平，流式细胞仪检测各组细胞的细胞凋亡率。结果 形态学检测显示，anti-IL-33能缓解经TNF-α处理后导致的FLS细胞变圆、间隙增加。CCK8法检测显示，与对照组比较，TNF-α（+）对照组细胞活力增加（P＜0.05）；与TNF-α（+）对照组比较，TNF-α（+）+anti-IL-33组和TNF-α（+）+甲氨蝶呤组细胞活力降低（P＜0.05）。凋亡检测显示，与对照组比较，TNF-α（+）对照组细胞凋亡降低（P＜0.05）；与TNF-α（+）对照组比较，TNF-α（+）+anti-IL-33组和TNF-α（+）+甲氨蝶呤组细胞凋亡率增高（P＜0.05）。结论 anti-IL-33可抑制RA-FLS增殖并诱导其凋亡，可为RA的治疗提供方向。
Abstract:
Objective To investigate the effect of anti-IL-33 antibody (anti-IL-33) on the proliferation and apoptosis of rheumatoid arthritis (RA) fibroblast-like synovial cells (FLS) and its mitigation and therapeutic effects in RA disease.Methods Cultivating human FLS, the experimental groups are as follows:Normal control group, TNF-α(+) control group, TNF-α(+) + anti-IL-33 (50 ng/ml) group, TNF-α(+) + methotrexate (1 μg/ml) group.Observing the morphology and structure of RA-FLS, and use CCK-8 method to determine the proliferation level of each group of cells, and flow cytometry to detect the apoptosis rate of each group of cells.Results Morphological examination showed that anti-IL-33 can alleviate the rounding and gap increase of FLS cells caused by TNF-α treatment.CCK8 method detection showed that compared with the control group, the cell viability of the TNF-α(+) control group increased (P＜0.05);Compared with the TNF-α(+) control group, the cell viability of the TNF-α(+)+anti-IL-33 group and the TNF-α(+)+ methotrexate group were decreased (P＜0.05).Apoptosis detection showed that compared with the control group, the apoptosis of the TNF-α(+) control group was reduced (P＜0.05);Compared with the TNF-α(+) control group, the apoptosis rate of the TNF-α(+)+anti-IL-33 group and the TNF-α(+)+methotrexate group was increased (P＜0.05).Conclusion anti-IL-33 can inhibit the proliferation of RA-FLS and induce its apoptosis, which can provide a direction for the treatment of RA.

参考文献/References:

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更新日期/Last Update: 1900-01-01