[1]童湘伟,石坤连,梁业婷,等.三七不同来源指纹图谱数字化研究[J].医学信息,2021,34(14):106-109,120.[doi:10.3969/j.issn.1006-1959.2021.14.028]
　TONG Xiang-wei,SHI Kun-lian,LIANG Ye-ting,et al.Research on Digitization of Fingerprints from Different Sources of Panax Notoginseng[J].Medical Information,2021,34(14):106-109,120.[doi:10.3969/j.issn.1006-1959.2021.14.028]
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三七不同来源指纹图谱数字化研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年14期
页码:
106-109,120
栏目:
论著
出版日期:
2021-07-15

文章信息/Info

Title:
Research on Digitization of Fingerprints from Different Sources of Panax Notoginseng
文章编号:
1006-1959（2021）14-0106-05
作者:
童湘伟; 石坤连; 梁业婷; 等
（1.涟源市人民医院药剂科，湖南 涟源 417100； 2.南方医科大学中医药学院，广东 广州 510515； 3.广西中医药大学药学院，广西 南宁 530001）
Author(s):
TONG Xiang-wei; SHI Kun-lian; LIANG Ye-ting; et al.
(1.The Pharmacy Department,People’s Hospital of Lianyuan City,Lianyuan 417100,Hunan,China; 2.School of Chinese Medicine,Southern Medical University,Guangzhou 510515,Guangdong,China; 3.School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China)
关键词:
三七; 指纹图谱; 数字化波动; 高效液相色谱法
Keywords:
Panax notoginseng; Fingerprint spectrum; Digital fluctuation; High performance liquid chromatography
分类号:
R917；O658
DOI:
10.3969/j.issn.1006-1959.2021.14.028
文献标志码:
A
摘要:
目的 初步探究三七药材指纹图谱数字化波动问题。方法 采用高效液相色谱法建立三七药材指纹图谱，色谱柱InertSustainTM AQ-C18，流动相为乙腈-0.1%磷酸溶液，梯度洗脱，流速1.0 ml/min，检测波长203 nm，柱温25 ℃，进样量10 μl，记录时间70 min，进行方法学考察；同时考察5批不同来源的三七指纹图谱，以探究指纹图谱的波动范围。结果 本方法成功建立了三七药材指纹图谱，相似度达到0.80以上，方法重复性、精密度、稳定性较好，各相对保留时间、相对峰面积的RSD均分别＜2.5%、＜3%、＜2.2%；初步确定三七指纹图谱的波动范围。结论 初步探究获得的三七药材指纹图谱波动范围对三七药材的质量控制研究提供了试验依据。
Abstract:
Objective To preliminarily explore the digital fluctuation problem of the fingerprint of Panax notoginseng medicinal material.Methods The fingerprint of Panax notoginseng was established by high performance liquid chromatography, and the column was InertSustainTM AQ-C18.The mobile phase is acetonitrile-0.1% phosphoric acid solution, gradient elution, flow rate 1.0 ml/min,the detection wavelength is 203 nm, the column temperature is 25 ℃, the injection volume is 10 μl, and the recording time is 70 min. Methodological investigation is carried out;At the same time, we investigated 5 batches of Panax notoginseng fingerprints from different sources to explore the fluctuation range of the fingerprints.Results The method successfully established the fingerprint of Panax notoginseng, and the similarity reached above 0.80. The method has good repeatability, precision and stability.The RSD of each relative retention time and relative peak area were <2.5%, <3%, and <2.2%, respectively;Preliminarily determine the fluctuation range of the notoginseng fingerprint.Conclusion The fluctuation range of the fingerprint of Panax notoginseng material obtained by preliminary exploration provides a test basis for the quality control research of Panax notoginseng material.

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更新日期/Last Update: 1900-01-01