[1]罗戈雯,何 杰,张 维.NAMPT在特发性肺纤维化血清和支气管肺泡灌洗液中的表达及其意义[J].医学信息,2021,34(18):66-69,77.[doi:10.3969/j.issn.1006-1959.2021.18.018]
 LUO Ge-wen,HE Jie,ZHANG Wei.The Expression of NAMPT in Serum and Bronchoalveolar Lavage Fluid ofPatients with Idiopathic Pulmonary Fibrosis and its Significance[J].Medical Information,2021,34(18):66-69,77.[doi:10.3969/j.issn.1006-1959.2021.18.018]
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NAMPT在特发性肺纤维化血清和支气管肺泡灌洗液中的表达及其意义()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年18期
页码:
66-69,77
栏目:
论著
出版日期:
2021-09-18

文章信息/Info

Title:
The Expression of NAMPT in Serum and Bronchoalveolar Lavage Fluid ofPatients with Idiopathic Pulmonary Fibrosis and its Significance
文章编号:
1006-1959(2021)18-0066-05
作者:
罗戈雯何 杰张 维
成都医学院临床医学院第一附属医院呼吸与危重症医学科,四川 成都 610500
Author(s):
LUO Ge-wenHE JieZHANG Wei
School of Clinical Medicine of Chengdu Medical College,Respiratory and Critical Care Medicine Department of the First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,Sichuan,China
关键词:
特发性肺纤维化烟酰胺磷酸核糖转移酶气管肺泡灌洗液
Keywords:
Idiopathic pulmonary fibrosisNicotinamide phosphoribosyl transferaseBronchoalveolar lavage fluid
分类号:
R563
DOI:
10.3969/j.issn.1006-1959.2021.18.018
文献标志码:
A
摘要:
目的 探讨烟酰胺磷酸核糖转移酶(NAMPT)在特发性肺纤维化(IPF)患者血清和支气管肺泡灌洗液中的表达水平及临床意义。方法 通过GEO数据库下载基因芯片数据集GSE53845、GSE24206、GSE10667,使用R软件筛选出正常肺组织与IPF组织中的差异表达基因,将差异基因与人类自噬基因数据库(HADb)中的自噬相关基因取交集,找到和IPF相关的自噬基因NAMPT。选择2018年1月-2020年3月于我科就诊的74例IPF患者为IPF组,另选取同期我院30例常规体检的健康者为对照组,检测两组人群支气管肺泡灌洗液(BALF)和血清中的NAMPT水平,分析两组的肺功能、呼吸困难评分(mMRC)、细胞学计数的差异以及血清和BALF中NAMPT水平与肺功能的相关性。结果 三个数据集与自噬相关基因取交集筛选出1个自噬相关基因NAMPT;IPF患者BALF中巨噬细胞百分数高于对照组,淋巴细胞百分数低于对照组,差异有统计学意义(P<0.05);IPF组血清和BALF中NAMPT水平均高于对照组,差异有统计学意义(P<0.05);IPF组患者BALF中NAMPT水平与FEV1%、FVC和DLCO%呈负相关(r=-0.687、-0.701、-0.622,P<0.05);血清中NAMPT水平与DLCO%呈负相关(r=-0.414,P<0.05);IPF组患者mMRC评分为(2.90±1.32)分,患者BALF和血清中的NAMPT水平与mMRC呈正相关(r=0.544,0.532,P<0.05)。结论 IPF患者NAMPT在BALF和血清中水平均增高,且与患者的肺功能相关;NAMPT水平可以用于IPF患者的病情监测以及疗效评估。
Abstract:
Objective To investigate the expression level and clinical significance of niacinamide phosphoribosyl transferase (NAMPT) in serum and bronchoalveolar lavage fluid of patients with idiopathic pulmonary fibrosis (IPF).Methods The gene chip dataset GSE53845, GSE24206 and GSE10667 were downloaded from GEO database, and the differentially expressed genes in normal lung tissues and IPF were screened by R software. The different genes were intermingled with autophagy-related genes in the human autophagy gene database (HADb) to find the IPF-related autophagy gene NAMPT. Seventy-four IPF patients admitted to our department from January 2018 to March 2020 were selected as the IPF group, and 30 healthy patients with routine physical examination were selected as the control group. The level of NAMPT in bronchoalveolar lavage fluid (BALF) and serum was detected,and analyzed the differences in lung function, dyspnea score(mMRC), cytological count and the correlation between the level of NAMPT in BALF and serum and lung function.Results One autophagy related gene NAMPT was selected from the intersection of three data sets and autophagy related genes. The percentage of macrophages in BALF in IPF group was significantly higher than that in the control group, the percentage of lymphocyte was lower than that of the control group (P<0.05). In both serum and BALF, the level of NAMPT in IPF group was significantly higher than that in the control group, and the difference was statistically significant (P<0.05). In the IPF group, the level of NAMPT in BALF was negatively correlated with FEV1%, FVC and DLCO% (r=-0.687,-0.701,-0.622,P<0.05); NAMPT in serum was negatively correlated with DLCO% (r=-0.414,P<0.05);in the IPF group, the mMRC score was (2.90±1.32) scores, while BALF and NAMPT in serum were positively correlated with mMRC (r=0.544,0.532,P<0.05).Conclusion The levels of Nampt in BALF and serum were increased in patients with IPF, and are related to pulmonary function. NAMPT level can be used to monitor the condition and evaluate the efficacy of IPF patients.

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更新日期/Last Update: 1900-01-01