[1]雷承泳,周志伟,段 彪,等.体外受精患者卵泡液高密度脂蛋白颗粒组分与胚胎质量的相关性分析[J].医学信息,2021,34(20):97-100.[doi:10.3969/j.issn.1006-1959.2021.20.024]
　LEI Cheng-yong,ZHOU Zhi-wei,DUAN Biao,et al.Correlation Between High Density Lipoprotein Granules in Follicular Fluid andEmbryo Quality in in Vitro Fertilization Patients[J].Medical Information,2021,34(20):97-100.[doi:10.3969/j.issn.1006-1959.2021.20.024]
点击复制

体外受精患者卵泡液高密度脂蛋白颗粒组分与胚胎质量的相关性分析()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年20期
页码:
97-100
栏目:
论著
出版日期:
2021-10-20

文章信息/Info

Title:
Correlation Between High Density Lipoprotein Granules in Follicular Fluid andEmbryo Quality in in Vitro Fertilization Patients
文章编号:
1006-1959（2021）20-0097-04
作者:
雷承泳; 周志伟; 段 彪; 罗朝霞; 冯 清
赣州市人民医院生殖医学科，江西 赣州 341000
Author(s):
LEI Cheng-yong; ZHOU Zhi-wei; DUAN Biao; LUO Zhao-xia; FENG Qing
Department of Reproductive Medicine,Ganzhou People’s Hospital,Ganzhou 341000,Jiangxi,China
关键词:
体外受精; 胚胎移植; 胚胎质量; 卵泡液; 高密度脂蛋白
Keywords:
In vitro fertilization; Embryo transfer; Embryo quality; Follicular fluid; High density lipoprotein
分类号:
R714
DOI:
10.3969/j.issn.1006-1959.2021.20.024
文献标志码:
A
摘要:
目的 探讨体外受精（IVF）患者卵泡液（FF）高密度脂蛋白（HDL）颗粒组分与卵裂期胚胎质量的相关性。方法 选择2019年8月-2020年3月在我科接受IVF-ET助孕的不孕妇女作为研究对象，共提取504份卵泡液样本，来自110例助孕患者。根据胚胎质量分为优质胚胎组（n=266）和非优质胚胎组（n=238）。测定卵泡液中载脂蛋白A1（ApoA1）和载脂蛋白B（ApoB）水平、HDL亚类水平、活性氧（ROS）和总抗氧化能力（TAC）水平，观察HDL颗粒组分ApoA1、HDL亚类水平与卵裂期胚胎质量的相关性。结果 两组ROS、TAC、ApoA1、ApoB、preβ1-HDL、HDL3c、HDL3b、HDL3a、HDL2a、HDL2b比较，差异有统计学意义（P＜0.05）；卵泡液中ROS、ApoB、preβ1-HDL、HDL3c、HDL3b水平与胚胎质量呈负相关（P＜0.05），ApoA1、HDL3a、HDL2a、HDL2b、TAC水平与胚胎质量呈正相关（P＜0.05）；多因素Logistic分析结果显示，ApoA1、HDL2b、ApoB为胚胎质量的影响因素（P＜0.05）。结论 HDL颗粒组分中ApoA1、HDL2b含量水平与IVF胚胎质量具有相关性，对胚胎质量具有一定的预测价值。
Abstract:
Objective To investigate the correlation between follicular fluid (FF) high density lipoprotein (HDL) granules composition and embryo quality in vitro fertilization (IVF) patients.Methods A total of 504 follicular fluid samples were collected from 110 infertile women undergoing IVF-ET in our department from August 2019 to March 2020. According to embryo quality, they were divided into high quality embryo group (n=266) and non-high quality embryo group (n=238). The levels of apolipoprotein A1 (ApoA1) and apolipoprotein B (ApoB), HDL subclasses, reactive oxygen species (ROS) and total antioxidant capacity (TAC) in follicular fluid were measured. The correlation between the levels of ApoA1, HDL subclasses of HDL granules and the quality of cleavage embryos was observed.Results The differences in ROS, TAC, ApoA1, ApoB, preβ1-HDL, HDL3c, HDL3b, HDL3a, HDL2a and HDL2b between the two groups were statistically significant (P＜0.05). The levels of ROS, ApoB, preβ1-HDL, HDL3c and HDL3b in follicular fluid were negatively correlated with embryo quality (P＜0.05). The levels of ApoA1, HDL3a, HDL2a, HDL2b and TAC were positively correlated with embryo quality (P＜0.05). Multivariate Logistic analysis showed that ApoA1, HDL2b and ApoB were the influencing factors of embryo quality (P＜0.05).Conclusion The level of ApoA1 and HDL2b in HDL granules are correlated with the quality of IVF embryos, and have certain predictive value for embryo quality.

参考文献/References:

[1]刘凯鲁,胡梦婷,蔡令波,等.多囊卵巢综合征患者卵泡液中6种miRNAs表达的检测[J].国际生殖健康/计划生育杂志,2018,37(1):5-10.[2]曾伟宏,李子涛,陈创奇,等.多囊卵巢综合征卵泡液代谢轮廓分析及对IVF-ET妊娠结局的影响[J].实用医学杂志,2018,34(19):73-77.[3]国家卫计委.卫生部关于修订人类辅助生殖技术与人类精子库相关技术规范、基本标准和伦理原则[R/OL].(2003-09-30)[2017-08-24].http://www.nhfpc.gov.cn/zwgkzt/wsbysj/200805/35747.shtml.[4]田莉峰,吴兴武,苏琼,等.早卵泡期超长方案鲜胚移植策略及临床妊娠结局分析[J].江西医药,2018,53(4):25-29.[5]吴欢,张静静,贺小进,等.不同手术取精方式在辅助生殖技术中的临床应用[J].当代医学,2018,24(2):180-182.[6]Kleijkers SH,Eijssen LM,Coonen E,et al.Differences in gene expression profiles between human preimplantation embryos cultured in two different IVF culture media[J].Hum Reprod,2015,30(10):2303-2311.[7]吴新伟,傅明德,刘秉文,等.人血清高密度脂蛋白亚类免疫印迹检测法[J].中国动脉硬化杂志,1999,7(3):253-255.[8]杨蕊,刘平,李蓉.专栏导读——辅助生殖技术提高配子胚胎质量、优化评估体系、改善临床结局系列研究[J].中华生殖与避孕杂志,2020,40(2):89-93.[9]张玲,熊承良.颗粒细胞和卵泡液因子在评估卵母细胞质量中的价值[J].中华生殖与避孕杂志,2010,30(12):827-831.[10]Várnagy á,Koszegi T,Gyorgyi E,et al.Levels of total antioxidant capacity and 8-hydroxy-2’-deoxyguanosine of serum and follicular fluid in women undergoing in vitrofertilization: focusing on endometriosis[J].Hum Fertil (Camb),2020,23(3):200-208.[11]Fisher JJ,Bartho LA,Perkins AV,et al.Placental mitochondria and reactive oxygen species in the physiology and pathophysiology of pregnancy[J].Clin Exp Pharmacol Physiol,2020,47(1):176-184.[12]Liu Y,Yu Z,Zhao S,et al.Oxidative stress markers in the follicular fluid of patients with polycystic ovary syndrome correlate with a decrease in embryo quality[J].J Assist Reprod Genet,2021,38(2):471-477.[13]王丽佳.多囊卵巢综合征病人卵泡液褪黑素与氧化应激的关联研究[J].安徽医药,2020,24(2):356-360.[14]Yilmaz N,Inal HA,Gorkem U,et al.Follicular fluid total antioxidant capacity levels in PCOS[J].J Obstet Gynaecol,2016,36(5):654-657.[15]Rincón JAA,Pradieé J,Remiao MH,et al.Effect of high-density lipoprotein on oocyte maturation and bovine embryo development in vitro[J].Reprod Domest Anim,2019,54(3):445-455.[16]Kim K,Bloom MS,Browne RW,et al.Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients[J].J Assist Reprod Genet,2017,34(1):1-10.[17]Takaeko Y,Matsui S,Kajikawa M,et al.Association of extremely high levels of high-density lipoprotein cholesterol with endothelial dysfunction in men[J].J Clin Lipidol,2019,13(4):664-672.

相似文献/References:

[1]马荣花,王莉云,沈源春,等.胚胎移植前宫腔内灌注hCG对FET反复着床失败患者 妊娠结局的影响[J].医学信息,2018,31(13):81.[doi:10.3969/j.issn.1006-1959.2018.13.023]
　MA Rong-hua,WANG Li-yun,SHEN Yuan-chun,et al.Effect of Intrauterine Perfusion of hCG before Embryo Transfer on Pregnancy Outcome in Patients with FET Repeated Implantation Failure[J].Medical Information,2018,31(20):81.[doi:10.3969/j.issn.1006-1959.2018.13.023]
[2]许菲菲,顾益惠,夏 飞.抑制素B在拮抗剂方案体外受精-胚胎移植中的变化及价值[J].医学信息,2022,35(07):79.[doi:10.3969/j.issn.1006-1959.2022.07.019]
　XU Fei-fei,GU Yi-hui,XIA Fei.Changes and Value of Inhibin B in Antagonist Protocol In Vitro Fertilization-embryo Transfer[J].Medical Information,2022,35(20):79.[doi:10.3969/j.issn.1006-1959.2022.07.019]
[3]覃 璧,韦霁芸,石德敏,等.不同促排卵方案在子宫内膜异位症合并不孕患者体外受精治疗中的效果比较[J].医学信息,2022,35(08):118.[doi:10.3969/j.issn.1006-1959.2022.08.029]
　QIN Bi,WEI Ji-yun,SHI De-min,et al.Comparison of the Effects of Different Ovulation Induction Regimens on In Vitro Fertilization in Patients with Endometriosis Complicated with Infertility[J].Medical Information,2022,35(20):118.[doi:10.3969/j.issn.1006-1959.2022.08.029]
[4]白雨昕.血清抗苗勒管激素对卵巢功能衰退及体外受精超促排卵中卵巢反应的预测价值[J].医学信息,2023,36(11):149.[doi:10.3969/j.issn.1006-1959.2023.11.031]
　BAI Yu-xin.Predictive Value of Serum Anti-Müllerian Hormone on Ovarian Function Decline and Ovarian Response in In Vitro Fertilization and Superovulation[J].Medical Information,2023,36(20):149.[doi:10.3969/j.issn.1006-1959.2023.11.031]

更新日期/Last Update: 1900-01-01