[1]岳永威,孔令军.儿童急性早幼粒细胞白血病的免疫表型特点分析[J].医学信息,2021,34(23):36-38.[doi:10.3969/j.issn.1006-1959.2021.23.010]
　YUE Yong-wei,KONG Ling-jun.Analysis of Immunophenotypic Characteristics of Children with Acute Promyelocytic Leukemia[J].Medical Information,2021,34(23):36-38.[doi:10.3969/j.issn.1006-1959.2021.23.010]
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儿童急性早幼粒细胞白血病的免疫表型特点分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年23期
页码:
36-38
栏目:
论著
出版日期:
2021-12-01

文章信息/Info

Title:
Analysis of Immunophenotypic Characteristics of Children with Acute Promyelocytic Leukemia
文章编号:
1006-1959（2021）23-0036-03
作者:
岳永威; 孔令军
（1.苏州大学附属儿童医院血液科，江苏 苏州 215000；2.苏州市吴江区儿童医院，江苏 苏州 215200）
Author(s):
YUE Yong-wei; KONG Ling-jun
(1.Department of Hematology,Children’s Hospital of Soochow University,Suzhou 215000,Jiangsu,China;2.Children’s Hospital of Wujiang District,Suzhou 215200,Jiangsu,China）
关键词:
急性早幼粒细胞白血病; FLT3-ITD; 免疫表型
Keywords:
Acute promyelocytic leukemia; FLT3-ITD; Immunophenotype
分类号:
R733.71
DOI:
10.3969/j.issn.1006-1959.2021.23.010
文献标志码:
A
摘要:
目的 分析儿童急性早幼粒细胞白血病（APL）的免疫表型特点。方法 选取2013年1月-2021年3月苏州大学附属儿童医院收治的87例儿童APL作为研究对象，结合FLT3-ITD突变及诊断时白细胞水平等相关数据，分析儿童APL的免疫表型特点及其在诊治中的应用价值。结果 APL细胞主要表达CD13、CD33、CD38、CD64、CD117、CD123、MPO，HLA-DR、CD7、CD2一般为阴性。高白细胞者与非高白细胞者CD117阳性、CD2阳性比较，差异有统计学意义（P＜0.05）。高危者与低危者CD34阳性、CD15阳性比较，差异无统计学意义（P＞0.05），而两者CD117阳性、CD2阳性比较，差异有统计学意义（P＜0.05）。FLT3-ITD突变者与无突变者CD2阳性、CD117阳性、CD11b阳性比较，差异有统计学意义（P＜0.05），而两者CD34阳性、CD15阳性比较，差异无统计学意义（P＞0.05）。结论 APL患儿早幼粒细胞免疫表型与成人类似，但CD34和CD11b的表达可能更高，且CD2阳性可能提示APL患儿预后不良，CD117阴性的APL患儿可能具有更高的白细胞水平，但具体的预后意义仍有待进一步研究。
Abstract:
Objective To analyze the immunophenotypic characteristics of childhood acute promyelocytic leukemia (APL).Methods A total of 87 children with APL admitted to Children’s Hospital of Soochow University from January 2013 to March 2021 were selected as the research objects, combined with relevant data such as FLT3-ITD mutation and white blood cell level at diagnosis, the immunophenotypic characteristics of childhood APL and its value in diagnosis and treatment were analyzed.Results APL cells mainly expressed CD13, CD33, CD38, CD64, CD117, CD123 and MPO, while HLA-DR, CD7 and CD2 were generally negative. There was a statistically significant difference in CD117 positive and CD2 positive between high leukocyte group and non-high leukocyte group (P<0.05). There was no significant difference in CD34 positive and CD15 positive between high-risk group and low-risk group (P>0.05), while there was significant difference in CD117 positive and CD2 positive between the two groups (P<0.05). There were statistically significant differences in CD2 positive, CD117 positive and CD11b positive between FLT3-ITD mutant and non-mutant (P<0.05), while there was no significant difference in CD34 positive and CD15 positive between the two groups (P>0.05).Conclusion The immunophenotype of promyelocytic in children with APL is similar to that of adults, but the expression of CD34 and CD11b may be higher. CD2 positive may indicate a poor prognosis in children with APL. Children with CD117-negative APL may have higher white blood cell levels, but the specific prognostic significance needs to be further analyzed.

参考文献/References:

[1]Zhang L,Zhu X.Epidemiology, diagnosis and treatment of acute promyelocytic leukemia in children: the experience in china[J].Mediterr J Hematol Infect Dis,2012,4(1):e2012012.[2]徐基昕,孔令军,蒋卫芹,等.儿童急性早幼粒细胞性白血病-2010方案单中心疗效分析[J].中华实用儿科临床杂志,2017,15(32):1166-1169.[3]马军,中华医学会血液学分会,中国医师协会血液科医师分会,等.中国急性早幼粒细胞白血病诊疗指南(2018年版)[J].中华血液学杂志,2018,39(3):179-183.[4]Horna P,Zhang L,Sotomayor EM,et al.Diagnostic immunophenotype of acute promyelocytic leukemia before and early during therapy with all-trans retinoic acid[J].Am J Clin Pathol,2014,142(4):546-552.[5]Chen Z,Li Y,Tong Y,et al.Stepwise discriminant function analysis for rapid identification of acute promyelocytic leukemia from acute myeloid leukemia with multiparameter flow cytometry[J].Int J Hematol,2016,103(3):306-315.[6]Del Principe MI,De Bellis E,Gurnari C,et al.Applications and efficiency of flow cytometry for leukemia diagnostics[J].Expert Rev Mol Diagn,2019,19(12):1089-1097.[7]Tran VT,Phan TT,Mac HP,et al.The diagnostic power of CD117, CD13, CD56, CD64, and MPO in rapid screening acute promyelocytic leukemia[J].BMC Res Notes,2020,13(1):394.[8]Gorczyca W.Acute promyelocytic leukemia: four distinct patterns by flow cytometry immunophenotyping[J].Pol J Pathol,2012,63(1):8-17.[9]Rahman K,Gupta R,Singh MK,et al.The triple-negative (CD34-/HLA-DR-/CD11b-) profile rapidly and specifically identifies an acute promyelocytic leukemia[J].Int J Lab Hematol,2018,40(2):144-151.[10]Juncà J,Garcia-Caro M,Granada I,et al.Correlation of CD11b and CD56 expression in adult acute myeloid leukemia with cytogenetic risk groups and prognosis[J].Ann Hematol,2014,93(9):1483-1489.[11]马荣军,朱尊民,袁晓莉,等.成人急性早幼粒细胞白血病预后相关因素分析[J].中华血液学杂志,2017,38(7):592-596.[12]Rizzatti EG,Garcia AB,Portieres FL,et al.Expression of CD117 and CD11b in bone marrow can differentiate acute promyelocytic leukemia from recovering benign myeloid proliferation[J].Am J Clin Pathol,2002,118(1):31-37.[13]Kaito K,Katayama T,Masuoka H,et al.CD2+ acute promyelocytic leukemia is associated with leukocytosis, variant morphology and poorer prognosis[J].Clin Lab Haematol,2005,27(5):307-311.[14]Testa U,Lo-Coco F.Prognostic factors in acute promyelocytic leukemia: strategies to define high-risk patients[J].Ann Hematol,2016,95(5):673-680.[15]陈芳,胡延平,王孝会,等.急性早幼粒细胞白血病免疫表型特点[J].中国实验血液学杂志,2016,24(2):321-325.[16]Nie L,Ma R,Yuan X,et al.The prognostic value of CD2, CD4, and HLA-DR expression and FLT3-ITD mutation in adult acute promyelocytic leukemia[J].Leuk Lymphoma,2020,61(10):2482-2487.[17]Wang D,Chen J,Ding Y,et al.miR-188-5p Promotes Tumor Growth by Targeting CD2AP Through PI3K/AKT/mTOR Signaling in Children with Acute Promyelocytic Leukemia[J].Onco Targets Ther,2020(13):6681-6697.[18]Sharawat SK,Gupta R,Raina V,et al.Increased coexpression of c-KIT and FLT3 receptors on myeloblasts: independent predictor of poor outcome in pediatric acute myeloid leukemia[J].Cytometry B Clin Cytom,2013,84(6):390-397.[19]Xu F,Yin CX,Wang CL,et al.Immunophenotypes and immune markers associated with acute promyelocytic leukemia prognosis[J].Dis Markers,2014(2014):421906.[20]van Solinge TS,Zeijlemaker W,Ossenkoppele GJ,et al.The interference of genetic associations in establishing the prognostic value of the immunophenotype in acute myeloid leukemia[J].Cytometry B Clin Cytom,2018,94(1):151-158.[21]Zhao J,Liang JW,Xue HL,et al.The genetics and clinical characteristics of children morphologically diagnosed as acute promyelocytic leukemia[J].Leukemia,2019,33(6):1387-1399.

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更新日期/Last Update: 1900-01-01