[1]李云霞,赵 玲,赵明利,等.奈达铂联合吉西他滨阻滞非霍奇金淋巴瘤细胞周期促进细胞凋亡的作用研究[J].医学信息,2022,35(08):61-66.[doi:10.3969/j.issn.1006-1959.2022.08.015]
 LI Yun-xia,ZHAO Ling,ZHAO Ming-li,et al.Effect of Nedaplatin Combined with Gemcitabine on Cell Cycle Arrest and Apoptosis in Non-Hodgkin Lymphoma[J].Medical Information,2022,35(08):61-66.[doi:10.3969/j.issn.1006-1959.2022.08.015]
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奈达铂联合吉西他滨阻滞非霍奇金淋巴瘤细胞周期促进细胞凋亡的作用研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年08期
页码:
61-66
栏目:
论著
出版日期:
2022-04-15

文章信息/Info

Title:
Effect of Nedaplatin Combined with Gemcitabine on Cell Cycle Arrest and Apoptosis in Non-Hodgkin Lymphoma
文章编号:
1006-1959(2022)08-0061-06
作者:
李云霞赵 玲赵明利
(昆明医科大学第三附属医院/云南省肿瘤医院内三科1,微创介入科2,云南 昆明 650118)
Author(s):
LI Yun-xiaZHAO LingZHAO Ming-liet al.
(The Third Department of Internal Medicine1,Department of Minimally Invasive Intervention2,the Third Affiliated Hospital ofKunming Medical University/Yunnan Cancer Hospital,Kunming 650118,Yunnan,China)
关键词:
奈达铂吉西他滨非霍奇金淋巴瘤细胞周期凋亡
Keywords:
NedaplatinGemcitabineNon-Hodgkin lymphomaCell cycleApoptosis
分类号:
R733.1
DOI:
10.3969/j.issn.1006-1959.2022.08.015
文献标志码:
A
摘要:
目的 利用两种非霍奇金淋巴瘤细胞株探究奈达铂(Nedaplatin)和吉西他滨(Gemcitabine)抑制细胞增殖的作用机制。方法 取MINO和SU-DHL-4非霍奇金淋巴瘤细胞株,分别设对照组、奈达铂组、吉西他滨组和奈达铂+吉西他滨组,对照组无药物处理,3个实验组的细胞分别用吉西他滨、奈达铂和奈达铂+吉西他滨处理,采用MTT实验比较各组细胞活力、流式细胞术检测各组细胞周期阻滞和细胞凋亡情况、免疫荧光检测各组细胞周期蛋白依赖激酶CDK1和CDK4的表达情况。结果 奈达铂组、吉西他滨组和奈达铂+吉西他滨组均抑制两种NHL细胞株的增殖,其中奈达铂+吉西他滨组抑制效果最显著;与对照组相比,3个实验组发生细胞周期阻滞,更多的细胞处于S期,其中奈达铂+吉西他滨组处于S期细胞比例最高;3个实验组可以诱导两种NHL细胞进入早期凋亡和晚期凋亡;免疫荧光结果显示,3个实验组CDK1和CDK4的表达低于对照组,且奈达铂+吉西他滨组降低CDK1和CDK4表达的幅度最大。结论 吉西他滨和奈达铂可通过下调细胞周期蛋白依赖激酶CDK1和CDK4的表达,引起细胞周期阻滞,从而诱导非霍奇金淋巴瘤细胞凋亡,最终抑制细胞增殖,发挥杀伤肿瘤的作用。
Abstract:
Objective To explore the inhibitory mechanism of Nedaplatin and Gemcitabine on cell proliferation by using two non-Hodgkin lymphoma cell lines.Methods MINO and SU-DHL-4 non-Hodgkinlymphoma cell lines were selected and divided into control group, Nedaplatin group, Gemcitabine group and Nedaplatin+Gemcitabine group. The control group was not treated with drugs. The cells in the three experimental groups were treated with Gemcitabine, Nedaplatin and Nedaplatin+Gemcitabine, respectively. MTT assay was used to compare the cell viability of each group, flow cytometry was used to detect cell cycle arrest and apoptosis, and immunofluorescence was used to detect the expression of cyclin-dependent kinase CDK1 and CDK4.Results Nedaplatin group, Gemcitabine group and Nedaplatin+Gemcitabine group all inhibited the proliferation of two NHL cell lines, and the inhibitory effect of Nedaplatin+Gemcitabine group was the most obvious. Compared with the control group, cell cycle arrest occurred in the three experimental groups, and more cells were in S phase, and the proportion of cells in S phase in Nedaplatin+Gemcitabine group was the highest. The three experimental groups could induce the early and late apoptosis of two NHL cells; immunofluorescence results showed that the expression of CDK1 and CDK4 in the three experimental groups were lower than that in the control group, and the Nedaplatin+Gemcitabine group decreased the expression of CDK1 and CDK4 most.Conclusion Gemcitabine and nedaplatin can induce cell cycle arrest by down-regulating the expression of cyclin-dependent kinases CDK1 and CDK4, thereby inducing apoptosis of non-Hodgkin lymphoma cells, and ultimately inhibiting cell proliferation.

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更新日期/Last Update: 1900-01-01