[1]宋 崟,张 科.基于生物信息学方法探究溃疡性结肠炎的关键基因和通路[J].医学信息,2022,35(16):13-17.[doi:10.3969/j.issn.1006-1959.2022.16.003]
　SONG Yin,ZHANG Ke.Key Genes and Pathways of Ulcerative Colitis Based on Bioinformatics[J].Journal of Medical Information,2022,35(16):13-17.[doi:10.3969/j.issn.1006-1959.2022.16.003]
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基于生物信息学方法探究溃疡性结肠炎的关键基因和通路()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年16期
页码:
13-17
栏目:
生物信息学
出版日期:
2022-08-15

文章信息/Info

Title:
Key Genes and Pathways of Ulcerative Colitis Based on Bioinformatics
文章编号:
1006-1959（2022）16-0013-05
作者:
宋 崟; 张 科
（天津医科大学总医院药剂科，天津 300052）
Author(s):
SONG Yin; ZHANG Ke
(Department of Pharmacy,Tianjin Medical University General Hospital,Tianjin 300052,China）
关键词:
溃疡性结肠炎; 差异基因; 信号通路; 吞噬体; 沙门氏菌感染
Keywords:
Ulcerative colitis; Differentially expressed genes; Signal pathway; Phagosome; Salmonella infection
分类号:
R737.25
DOI:
10.3969/j.issn.1006-1959.2022.16.003
文献标志码:
A
摘要:
目的 探究溃疡性结肠炎（UC）的发病机制，为其早期诊断和治疗药物的开发提供新思路。方法 从GEO数据库中下载基因芯片GSE42911数据集，使用在线分析工具GEO2R筛选出UC患者与健康人肠组织的差异表达基因（DEGs），将DEGs导入STRING数据库进行分析，筛选核心DEGs；利用在线数据库 DAVID 6.8和 STRING 11.0分别对核心DEGs进行基因本体论分析（GO）、京都基因和基因组百科全书（KEGG）通路富集分析和蛋白互作分析（PPI），使用 Cytoscape 3.2.1软件筛选蛋白相互作用网络中的核心节点。结果 共筛选出487个DEGs，其中465个上调基因，22个下调基因，进一步筛选得到36个核心DEGs；KEGG 富集分析显示，36个核心DEGs富集在25条通路上，主要包括：吞噬体、黏着小带、细菌侵袭上皮细胞、沙门氏菌感染、肌动蛋白细胞骨架调节、白细胞内皮移动等；GO分析显示主要富集的生物工程包括：GTP酶介导的信号转导、细胞或亚细胞成分的运动、线粒体ATP合成耦合质子转运、ATP合成耦合质子运输、线粒体膜电位的调节、胞外外体、胞质溶胶、细胞外间质、局部粘连、蛋白质结合、多聚RNA结合、质子转运ATP合成酶活性、谷胱甘肽酶活性、跨膜转运活性等；PPI 网络分析得到10核心基因：RACK1、ACTG1、UBB、CCT8、HSPD1、SOD1、CDC42、PSMA6、CFL1、RPL7。结论 RACK1、ACTG1、UBB、CCT8等基因可能通过调控吞噬体、黏着小带、细菌侵袭上皮细胞、沙门氏菌感染、肌动蛋白细胞骨架调节、白细胞内皮移动等信号通路参与溃疡性结肠炎的发生发展。
Abstract:
Objective To explore the pathogenesis of ulcerative colitis ( UC ) and provide new ideas for its early diagnosis and development of therapeutic drugs.Methods The gene chip GSE42911 data set was downloaded from the GEO database, and the online analysis tool GEO2 R was used to screen the differentially expressed genes (DEGs) in the intestinal tissues of UC patients and healthy people. The DEGs were imported into the STRING database for analysis to screen the core DEGs. Gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and protein-protein interaction (PPI) analysis were performed on the core DEGs using the online databases DAVID 6.8 and STRING 11.0, respectively. Cytoscape 3.2.1 software was used to screen the core nodes in the PPI network.Results A total of 487 DEGs were screened, including 465 up-regulated genes and 22 down-regulated genes, and 36 core DEGs were further screened. KEGG enrichment analysis showed that 36 core DEGs were enriched in 25 pathways, including phagosome, adhesive band, bacterial invasion of epithelial cells, Salmonella infection, actin cytoskeleton regulation, leukocyte endothelial movement, etc. GO analysis showed that the main enriched biotechnologies included GTPase-mediated signal transduction, cell or subcellular component movement, mitochondrial ATP synthesis coupled proton transport, ATP synthesis coupled proton transport, mitochondrial membrane potential regulation, extracellular exosomes, cytoplasmic sol, extracellular matrix, local adhesion, protein binding, poly RNA binding, proton transport ATP synthase activity, glutathione enzyme activity, transmembrane transport activity, etc. PPI network analysis obtained 10 core genes:RACK1, ACTG1, UBB, CCT8, HSPD1, SOD1, CDC42, PSMA6, CFL1, RPL7.Conclusion RACK1, ACTG1, UBB, CCT8 and other genes may be involved in the occurrence and development of ulcerative colitis by regulating phagosome, adhesive band, bacterial invasion of epithelial cells, Salmonella infection, actin cytoskeleton regulation, leukocyte endothelial migration and other signaling pathways.

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更新日期/Last Update: 1900-01-01